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| 1 | Dysfunction of peripheral blood dendritic cells from patients with chronic hepatitis B virus infection显示文摘AIM To identify the property of dendritic cella (DCs) of peripheral blood monocytes (PBMC) in patlents with chronic HBV infection.METHODS Twenty patients with persistent HBV infectlon were included in this study, 10 healthy subjects being used as a control group. The peripheral blood mononuclear cells (PBMC) of T cell-depleted populations were incubated and induced into mature dendritic cells in the RPMI-1640 medium in the presence of cytokines GMCSF, IL-4, FLt-3, TNF-α and 100 mL@ L-1 of fetal calf serum for a total of 10 - 12 days. The expressions of surface markers on DCs were evaluated using flow cytometric analysis. ELISA method was used to determine the cytokine levels of interleukin-12 (IL-12) and IL-10 in the supernatant produced by DCs. For detection of the stimulatory capacity of DCs to T cell proliferation,mytomycin C-treated DC were incubated with allogenic T cells.RESULTS A typical morphology of mature DCs from healthy subjects and HBV-infected patients was induced in in vitro incubation, but the proliferation ability and cellular number of DCs from HBV-infected patients significantly decreased compared with healthy individuals. In particular, the expression levels of HLADR, CD80 (B7-1) and CD86 (B7-2) on DC surface from patients were also lower than that from healthy individuals (0.46 vs 0.92 for HLA-DR, 0.44 vs 0.88 for CD80 and 0.44 vs 0. 84 for CD86, P< 0.05). The stimulatory capacity and production of IL-12 of DCs from patients in allogenic mixed lymphocyte reaction (AMLR) significantly decreased, but the production level of nitric oxide (NO) by DCa simultaneously increased compared with healthy subjects (86± 15 vs 170±22 μmoI@L 1, P<0.05).CONCLUSION The patients with chronic HBV infection have the defective function and immature phenotype of dendritic cells, which may be associated with the inability of efficient presentation of HBV antigens to host immune system for the clearance of HBV. | Fu-Sheng Wang Li-He Xing Ming-Xu Liu Chuan-Lin Zhu Hui-Gang Liu Hui-Fen Wang Zhou-Yun Lei Division of Biological Engineering,~2 Fourth Department of Liver Diseases,Beijing Institute of Infectious Diseases,Beijing Hospital of Infectious Diseases,Beijing 100039,China | 2001 | World Journal of Gastroenterology2001，7，4： | 131 |
| 2 | Distribution of nitric oxide synthase in stomach myenteric plexus of rats显示文摘AIM: To study the distribution of nitric oxide synthase (NOS) in rat stomach myenteric plexus.METHODS: The distribution of NOS in gastric wall was studied in quantity and location by the NADPH-diaphorase (NDP) histochemical staining method and whole mount preparation technique.RESULTS: NOS was distributed in whole stomach wall, most of them were located in myenteric plexus, and distributed in submucosal plexus. The shape of NOS positive neurons was basically similar, most of them being round and oval in shape. But their density, size and staining intensity varied greatly in the different parts of stomach. The density was 62 -± 38 cells/mm2(antrum), 43 ± 32 cells/mm2(body), and 32 ± 28 cells/mm2 (fundus), respectively. The size and staining intensity of NOS positive neurons in the fundus were basically the same, the neurons being large and dark stained, while they were obviously different in antrum. In the body of the stomach, the NOS positive neurons were in an intermediate state from fundus to antrum. There were some beadlike structures which were strung together by NOS positive varicosities in nerve fibers, some were closely adherent to the outer walls of blood vessels.CONCLUSION: Nitric oxide might he involved in the modulation of motility, secretion and blood ciroulation of the stomach, and the significant difference of NOS positive neurons in different parts of stomach myenteric plexus may be related to the physiologic function of stomach. | Xi Peng Jin-Bin Feng Hong Yan Yun Zhao Shi-Liang Wang Institute of Burn Research,Southwest Hospital,Third Military Medical University,Chongqing 400038,China | 2001 | World Journal of Gastroenterology2001，7，6： | 11 |
| 3 | Relationship between genotype and phenotype of flagellin C in Salmonella显示文摘AIM: To discover the relationship between the genotype and antigen serotype of flagellin C among Salmonella strains.METHODS: Fragment of Salmonella flagellin C in plasmid pLS408 was cloned, sequenced and compared with the corresponding sequence in other strains. Salmonella strains including two typhi strains, one paratyphoid strain, one enteritidis and one typhimurium strain were isolated from outpatients. Genome DNA was purified respectively from these clinical isolstes, then the corresponding flagellin C fragment was amplified by polymerase chain reaction, and the amplification products were analyzed by agarose gel electrophoreeis.RESULTS: The cloned fragment includes 582 nucleotides encoding the variable region and partial conservative region of Salmonella flagellin C in plasmid pLS408. With comparison to the corresponding sequences reported previously, there is only a little difference from other strains with the same flagellar serotype in both nucleotide and amino acid level. Specific PCR products were amplified in Salmonella strains with flagellar eerotype H-1-d including S. Muenchen, typhi and typhimurium, but not in S.paratyphoid C or S. Enteritidis strains.CONCLUSION: In this experiment, the specificity of nucleotide sequence could be found in flagellin C central variable regions as it exists in flagellar serotypes in Salmonella. It may be helpful to developing a rapid,sensitive, accurate and PCR-based method to detect Salmonella strains with serotype H-1-d. | Wan-Sheng Ji~1 Jia-Lu Hu~1 Jun-Wen Qiu~1 Bo-Rong Pan~1 Dao-Rong Peng~2 Bing-Long Shi~1 Shao-Juan Zhou~1 Kai-Chun Wu~1 Dai-Ming Fan~1 1 Chinese PLA Institute of Digestive Diseases2 Department of Bacteriology,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2001 | World Journal of Gastroenterology2001，7，6： | 2 |
| 4 | 抗体封闭趋化蛋白受体5对1型糖尿病发病的影响显示文摘目的 :研究体内封闭趋化蛋白受体 5 (CCR5 )分子对严重联合免疫缺陷小鼠 (severe combined im munodeficientNOD,NOD.Scid) 1型糖尿病发病的影响。 方法 :将发病非肥胖性糖尿病小鼠 (1只 )的脾细胞腹腔注射至 NOD.Scid小鼠体内 ,将这些小鼠随机分成 2组 (每组 8只 ) :抗 CCR5组小鼠腹腔注射抗 CCR5膜外第一襻的多克隆抗体 ,1周 3次 ,共计 12次 ;对照组相同方法注射 PBS,同时测定其血糖和体重。脾细胞注射后 70 d或当小鼠出现重度糖尿病临床表现时处死 ,进行组织学观察和脾细胞分泌细胞因子的 EL ISA测定。 结果 :脾细胞射后 70 d内 ,抗 CCR5组小鼠糖尿病发病率为 37.5 % (3/ 8) ,对照组为 10 0 % (8/ 8) ;胰腺炎积分抗 CCR5组较对照组差异显著 (P<0 .0 5 ) ;抗 CCR5组 IL- 2、IL- 10、IFN- γ水平分别为 2 5 .2、2 7.3pg/ ml和 0 .2 38ng/ ml,对照组为 5 7.7、31.6 pg/ ml和 18.0 3ng/ ml,前者的 IL- 2和 IFN- γ水平显著低于后者 (P<0 .0 5 )。结论 :在 NOD.Scid小鼠体内注射抗体封闭 CCR5可以部分抑制 1型糖尿病的发生。 | 冯皓 郭葆玉 | 2004 | 第二军医大学学报2004，25，10： | 1 |
| 5 | Sequence of a new prothymosinα cDNA显示文摘Objective:To clone prothymosin α which has important biological functions and to analyze its sequence. Methods:huPTMA α cDNA was cloned from poly(A) + RNA of the healthy Chinese peripheral blood monocyte cells by RT-PCR and the sequence was analyzed. Results:It was a new subtype of huPTMA α and has 86.4% homology with Genbank data. Conclusion:We find a new gene from the healthy Chinese, and it is named and accepted by Genbank. | 郭葆玉 余建国 张淑英 道书艳 苗红 邱磊 | 2000 | Journal of Medical Colleges of PLA(China)2000，15，3： | 0 |
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