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| 1 | Excretion of manure-borne estrogens and androgens and their potential risk estimation in the Yangtze River Basin显示文摘The Yangtze River is the longest river in China, and the river basin spans one fifth of the area of the whole country. Based on statistical data, the excretion of manure-borne steroid hormones, including steroid estrogens(SEs) and steroid androgens(SAs), in 10 provinces of China within the region has been estimated. The potential environmental and ecological risk of manure-borne steroid estrogens to the surface water in this region was also assessed. The manure-borne SE and SA excretions in the 10 provinces and municipalities vary in the order: Sichuan > Hunan > Hubei > Yunnan > Jiangsu > Anhui > Jiangxi >Chongqing > Qinghai > Shanghai. The highest increase of manure-borne SEs(1434.3 kg)and SAs(408.5 kg) was found in Hunan and Hubei provinces, respectively, and the total excretion in 2013 was 65% more than 15 years earlier in these two provinces. However, the emissions in Anhui and Shanghai decreased in this 15 year period of time. Swine urine,chicken feces, cattle urine, and laying hen feces were considered the dominant sources of manure-borne E1, βE2, αE2, and SAs, respectively. Although Jiangsu province did not have the largest excretion of manure-borne SEs, it had the highest level of predicted17β-estradiol equivalency(EEQs) value of 16.65 ng/L in surface water because of the limited surface water resources. According to the lowest observable effect level of 10 ng/L for17β-estradiol, the manure-borne SEs in Jiangsu province might potentially pose ecological risk to its wild aquatic organisms. | Yanxia Li Shiying Gao Shufang Liu Bei Liu Xuelian Zhang Min Gao Linjie Cheng Boyang Hu | 2015 | Journal of Environmental Sciences2015，27，11： | 2 |
| 2 | 利用罗非鱼卵黄原蛋白建立雌激素污染的检测方法显示文摘目的以尼罗罗非鱼为实验对象,以卵黄原蛋白(Vg)为生物标志物开发环境雌激素的生物检测技术。方法采用凝胶过滤与离子交换层析相结合的方法从17β-雌二醇诱导后的罗非鱼血浆中分离纯化Vg,对纯化的蛋白进行鉴定后制备多克隆抗血清,建立Vg的酶联免疫吸附测定实验(ELISA),并用于样品测定。结果建立的ELISA工作范围为15.6~1000 ng/m L,组内与组间差异分别为6.85%与6.79%,能有效检测100、500、1000μg/L双酚A对雄鱼血浆Vg的诱导效应。结论本研究建立的Vg ELISA具有较高的敏感度、特异性与精确度,可为水体环境雌激素污染检测提供重要工具。 | 王松 王骏 贾俊涛 吕宁 姜英辉 | 2016 | 食品安全质量检测学报2016，7，4： | 1 |
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