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| 1 | Correlation of fibrinogen-like protein 2 with progression of acute pancreatitis in rats显示文摘AIM: To examine fibrinogen-like protein 2 (fgl2) expression during taurocholate-induced acute pancreatitis progression in rats and its correlation with pancreatic injury severity. METHODS: Forty-eight male Sprague-Dawley rats were randomly divided into the severe acute pancreatitis (SAP) group (n = 24) and the sham operation (SO) group (n = 24). Sodium taurocholate (4% at doses of 1 mL/kg body weight) was retrogradely injected into the biliopancreatic ducts of the rats to induce SAP. Pancreatic tissues were prepared immediately after sacrifice. At the time of sacrifice, blood was obtained for determination of serum amylase activity and isolation of peripheral blood mononuclear cells (PBMCs). Pancreatic tissue specimens were obtained for routine light microscopy including hematoxylin and eosin staining, and the severity of pancreatic injury was evaluated 1, 4 and 8 h after induction. Expression of fgl2 mRNA was measured in the pancreas and PBMCs using reverse transcription polymerase chain reaction. Expression of fgl2 protein was evaluated in pancreatic tissues using Western blotting and immunohistochemical staining. Masson staining was also performed to observe microthrombosis. RESULTS: At each time point, levels of fgl2 mRNAs in pancreatic tissues and PBMCs were higher (P < 0.05) in the SAP group than in the SO group. For pancreatic tissue in SAP vs SO, the levels were: after 1 h, 3.911 ± 1.277 vs 1.000 ± 0.673; after 4 h, 9.850 ± 3.095 vs 1.136 ± 0.609; and after 8 h, 12.870 ± 3.046 vs 1.177 ± 0.458. For PBMCs in SAP vs SO, the levels were: after 1 h, 2.678 ± 1.509 vs 1.000 ± 0.965; after 4 h, 6.922 ± 1.984 vs 1.051 ± 0.781; and after 8 h, 13.533 ± 6.575 vs 1.306 ± 1.179. Levels of fgl2 protein expression as determined by Western blotting and immunohistochemical staining were markedly up-regulated (P < 0.001) in the SAP group compared with those in the SO group. For Western blotting in SAP vs SO, the results were: after 1 h, 2.183 ± 0.115 vs 1.110 ± 0.158; after 4 h, 2.697 ± 0.090 vs 0.947 ± 0.361; and after 8 h, 3.258 ± 0.094 vs 1.208 ± 0.082. For immunohistochemical staining in SAP vs SO, the results were: after 1 h, 1.793 ± 0.463 vs 0.808 ± 0.252; after 4 h, 4.535 ± 0.550 vs 0.871 ± 0.318; and after 8 h, 6.071 ± 0.941 vs 1.020 ± 0.406. Moreover, we observed a positive correlation in the pancreas (r = 0.852, P < 0.001) and PBMCs (r = 0.735, P < 0.001) between fgl2 expression and the severity of pancreatic injury. Masson staining showed that microthrombosis (%) in rats with SAP was increased (P < 0.001) compared with that in the SO group and it was closely correlated with fgl2 expression in the pancreas (r = 0.842, P < 0.001). For Masson staining in SAP vs SO, the results were: after 1 h, 26.880 ± 9.031 vs 8.630 ± 3.739; after 4 h, 53.750 ± 19.039 vs 8.500 ± 4.472; and after 8 h, 80.250 ± 12.915 vs 10.630 ± 7.003.CONCLUSION: Microthrombosis due to fgl2 overexpression contributes to pancreatic impairment in rats with SAP, and fgl2 level may serve as a biomarker during early stages of disease. | Xiao-Hua Ye Tan-Zhou Chen Jia-Ping Huai Guang-Rong Lu Xiao-Ju Zhuge Ren-Pin Chen Wu-Jie Chen Chen Wang Zhi-Ming Huang | 2013 | World Journal of Gastroenterology2013，19，16： | 4 |
| 2 | 女性糖尿病周围神经病变相关基因筛选及生物信息学分析显示文摘目的通过对GEO数据库中糖尿病周围神经病变(DPN)相关基因芯片进行生物信息学分析,获取DPN关键基因及信号通路。方法在GEO数据库中下载DPN相关基因芯片,利用R语言分析DPN女性患者与正常对照组的差异基因(DEGs)并进行可视化,根据基因本体论(GO)和京都基因与基因组百科全书(KEGG)对差异基因进行注释,预测其功能与相关通路,利用STRING数据库构建蛋白质相互作用网络筛选核心基因。结果分析芯片GSE95849获取差异基因4746个,其中上调基因2218个,下调基因2528个。其中TFAP2C、ESR1、CX3CR1、FGL2处于蛋白质相互作用核心位点。结论差异基因主要参与MAPK通路,通过血糖稳态、炎症作用、神经元发育等参与DPN发病过程,为DPN的诊断及治疗提供新的思路。 | 张艺 邱珍 夏中元 | 2020 | 海南医学院学报2020，26，1： | 3 |
| 3 | fgl2凝血酶原酶临床应用基础研究进展显示文摘fgl2凝血酶原酶是新近发现的凝血因子,主要表达于外周血T淋巴细胞、活化的内皮细胞和巨噬细胞等免疫活性细胞,其不依赖经典内、外源性凝血途径,可直接激活凝血酶原,启动凝血反应。近年来随着对fgl2凝血酶原酶日益关注,相关研究渗透到临床各专业,为阻断、延缓疾病的发生、发展和新药开发提供了靶向,为防治疾病带来新希望。该文将近几年fgl2凝血酶原酶在消化、呼吸、泌尿、心血管及风湿免疫系统等的临床应用基础研究做一综述,作为研究者进一步深入探索的启发和参考。 | 黄芳 徐艳秋 王怡 | 2012 | 安徽医药2012，16，7： | 2 |
| 4 | fgl2凝血酶原酶在重症急性胰腺炎大鼠肝损伤的表达意义显示文摘目的探讨重症急性胰腺炎(SAP)大鼠肝损伤中fgl2凝血酶原酶的表达情况及意义。方法 SD大鼠采用数表法随机均分为假手术(SO)组和SAP组,各24只。采用逆行胆胰管注射4%牛磺胆酸钠法制备大鼠SAP模型。收集术后1、4及8h时点肝脏标本,行常规病理检查及Masson染色随机计数肝脏微血管内微血栓形成的阳性血管数;血样检测丙氨酸氨基转移酶(ALT)及天冬氨酸氨基转移酶(AST);实时定量(real-time)PCR法、免疫组织化学法测定fgl2凝血酶原酶在大鼠肝脏的表达情况。结果组织病理学检查示SAP大鼠肝组织内炎细胞浸润及坏死;fgl2凝血酶原酶在SAP大鼠肝脏微血管内皮细胞高表达(P<0.01);SAP组肝脏微血管可见微血栓形成;SAP大鼠阳性血管率显著高于SO组(P<0.01),且与肝脏fgl2凝血酶原酶表达呈正相关(r=0.948,P<0.01);SAP组肝脏病理学评分显著高于SO组(P<0.01)且与fgl2凝血酶原酶表达呈正相关(r=0.704,P<0.01)。结论 fgl2凝血酶原酶在SAP大鼠肝脏中的异常表达可能通过介导微血栓形成导致SAP相关性肝损伤的进展;其表达水平可能与SAP相关性肝损伤的病理损害程度相关。 | 叶晓华 怀佳萍 丁进 黄智铭 | 2013 | 医学研究杂志2013，42，12： | 1 |
| 5 | 益肾泄浊方对高糖环境下人肾小球内皮细胞的保护作用及机制显示文摘目的:探讨益肾泄浊方含药血清对高糖环境下人肾小球内皮细胞的保护作用及其机制。方法:体外培养的人肾小球内皮细胞分为空白组、模型组、空白血清对照组、益肾泄浊方含药血清低剂量组、益肾泄浊方含药血清高剂量组,每组设置3个复孔。除空白组外,每组给予含30 mmol·L^(-1) D-葡萄糖的ECM培养基培养2 h后,给予相应药物进行干预,分别于干预24 h、48 h后,CCK-8法检测各组细胞的增殖情况;ELISA法检测细胞上清中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、纤维蛋白原样蛋白2(fibrinogen-like protein2,fgl2)凝血酶原酶水平;RT-PCR法检测细胞中TNF-α mRNA、fgl2凝血酶原酶mRNA的表达情况;分析细胞上清中TNF-α与fgl2凝血酶原酶水平的相关性及细胞中TNF-α mRNA与fgl2凝血酶原酶mRNA表达的相关性。结果:与空白组比较,模型组肾小球内皮细胞的增殖率显著降低(P<0.05),细胞上清中TNF-α、fgl2凝血酶原酶水平及细胞中TNF-α mRNA、fgl2凝血酶原酶mRNA表达水平显著升高(P<0.05);与模型组比较,益肾泄浊方能显著提高人肾小球内皮细胞增殖率(P<0.05),显著降低细胞上清中TNF-α、fgl2凝血酶原酶水平及细胞中TNF-α mRNA、fgl2凝血酶原酶mRNA表达水平(P<0.05),且细胞上清中TNF-α与fgl2凝血酶原酶水平及两者mRNA表达水平呈正相关关系。结论:益肾泄浊方含药血清能保护高糖环境下肾小球内皮细胞损伤,其可能的作用机制是通过纠正由炎症因子TNF-α介导的免疫凝血功能异常,缓解肾脏微循环障碍,保护肾脏功能。 | 王宪赟 黄芳 张小鹿 王怡 | 2021 | 中医学报2021，36，9： | 1 |
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