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| 1 | 454测序技术开发微卫星标记的研究进展显示文摘第二代测序技术(454测序为例)已成为测定基因组序列的一种成熟技术。454测序亦可应用于目标DNA区域分析,因此可用作微卫星标记的开发。较传统方法而言,具有便捷、高效等特点。目前,运用454技术进行基因组测序或转录组测序开发微卫星标记,用以研究种群生态学、构建遗传图谱等得到越来越多的重视和应用。综述了454测序技术在开发微卫星标记上的应用,并根据其优缺点对其应用前景进行了展望,旨在为应用454测序开发微卫星提供参考。 | 程晓凤 黄福江 刘明典 汪登强 | 2011 | 生物技术通报2011，27，8： | 15 |
| 2 | Recent Progress Using High-throughput Sequencing Technologies in Plant Molecular Breeding显示文摘High-throughput sequencing is a revolutionary technological innovation in DNA sequencing.This technology has an ultra-low cost per base of sequencing and an overwhelmingly high data output.High-throughput sequencing has brought novel research methods and solutions to the research fields of genomics and post-genomics.Furthermore,this technology is leading to a new molecular breeding revolution that has landmark significance for scientific research and enables us to launch multi-level,multi-faceted,and multi-extent studies in the fields of crop genetics,genomics,and crop breeding.In this paper,we review progress in the application of high-throughput sequencing technologies to plant molecular breeding studies. | Qiang Gao Guidong Yue Wenqi Li Junyi Wang Jiaohui Xu Ye Yin | 2012 | Journal of Integrative Plant Biology2012，54，4： | 15 |
| 3 | 胡枝子叶片转录组特征分析及其EST-SSR标记开发(英文)显示文摘胡枝子(Lespedeza bicolor)系豆科胡枝子属植物,性耐旱,是防风固沙及水土保持的优良植物.报道胡枝子的转录组序列,对其进行注释,并开发一系列EST-SSR标记用于进化研究.主要研究结果如下:(1)采用二代测序技术对胡枝子叶片进行转录组测序,得到120 913 unigenes,其平均长度为608 bp,N50的长度是978 bp.(2)分别在KEGG和KOG等数据库中对unigenes进行注释,总共注释72 613(60.05%)unigenes的功能.(3)筛选识别13 551个潜在的EST-SSR标记;根据重复单位的核苷酸数目以及重复次数的多少,从中选择173个EST-SSR标记进行引物设计.(4)对胡枝子和其他9种近缘种植物进行PCR扩增实验,最后得到56对引物可全部成功扩增出条带并表现出一定的多态性.本研究获得了较高质量的胡枝子转录组数据库,可进一步用于比较和功能基因组研究以及胡枝子及其近缘类群的基因表达研究;开发的EST-SSR标记将提供一个强大的工具,可用于研究遗传多样性和种群结构、构建DNA指纹图谱数据库、生成遗传图谱、预测分子标记辅助育种和保存遗传信息. | 李林霖 高信芬 李诗琦 陈金元 郭丽娜 何海 徐波 | 2018 | 应用与环境生物学报2018，24，6： | 1 |
| 4 | Analysis of simple sequence repeats in rice bean(Vigna umbellata) using an SSR-enriched library显示文摘Rice bean(Vigna umbellata Thunb.), a warm-season annual legume, is grown in Asia mainly for dried grain or fodder and plays an important role in human and animal nutrition because the grains are rich in protein and some essential fatty acids and minerals. With the aim of expediting the genetic improvement of rice bean, we initiated a project to develop genomic resources and tools for molecular breeding in this little-known but important crop.Here we report the construction of an SSR-enriched genomic library from DNA extracted from pooled young leaf tissues of 22 rice bean genotypes and developing SSR markers.In 433,562 reads generated by a Roche 454 GS-FLX sequencer, we identified 261,458 SSRs, of which 48.8% were of compound form. Dinucleotide repeats were predominant with an absolute proportion of 81.6%, followed by trinucleotides(17.8%). Other types together accounted for 0.6%. The motif AC/GT accounted for 77.7% of the total, followed by AAG/CTT(14.3%), and all others accounted for 12.0%. Among the flanking sequences, 2928 matched putative genes or gene models in the protein database of Arabidopsis thaliana, corresponding with 608 non-redundant Gene Ontology terms. Of these sequences, 11.2% were involved in cellular components, 24.2% were involved molecular functions, and 64.6% were associated with biological processes. Based on homolog analysis, 1595 flanking sequences were similar to mung bean and 500 to common bean genomic sequences. Comparative mapping was conducted using 350 sequences homologous to both mung bean and common bean sequences. Finally, a set of primer pairs were designed, and a validation test showed that58 of 220 new primers can be used in rice bean and 53 can be transferred to mung bean.However, only 11 were polymorphic when tested on 32 rice bean varieties. We propose that this study lays the groundwork for developing novel SSR markers and will enhance the mapping of qualitative and quantitative traits and marker-assisted selection in rice bean and other Vigna species. | Lixia Wang Kyung Do Kim Dongying Gao Honglin Chen Suhua Wang Suk Ha Lee Scott A. Jackson Xuzhen Cheng | 2016 | The Crop Journal2016，4，1： | 0 |
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