| 1 | 不同减肥方式对雄性肥胖小鼠脂肪组织炎症-芳香化酶轴的影响显示文摘目的:探究有氧运动或降脂饮食干预对雄性肥胖小鼠脂肪组织雄激素芳香化作用的影响。方法:3周龄雄性C57BL/6小鼠,按体重随机分为正常饮食组(N组,n=8)和高脂饮食组(F组,n=32)。其中N组正常饮食,F组为高脂饮食。造肥胖模型成功,剔除肥胖未达标小鼠8只后,F组按体重随机分为高脂饮食对照组(FO组,n=8)、高脂饮食运动组(FE组,n=8)、高脂饮食转正常饮食组(FN组,n=8)。FO组持续高脂饮食,不运动。FE组高脂饮食,进行中等强度跑台运动,跑速、运动时间和频率递增,3后周恒定为跑速20 m/min,每天运动60 min,每周运动6天。FN组改为正常饮食,不运动。运动和饮食干预为期8周。末次运动后36~40小时,并禁食12小时后,称量体重,测量体长,取眼球血、腹腔脂肪和腓肠肌。计算Lee’s指数、体脂百分比和肌肉质量指数。ELISA法测试血清和脂肪组织睾酮、雌二醇、白介素6(IL6)、肿瘤坏死因子α(TNFα)。Western Blot法测试脂肪组织芳香化酶、IL6受体α(IL6-Rα)、TNF受体1(TNF-R1)的蛋白表达。结果:(1)FO组血清和脂肪组织IL6和TNFα高于N组(P<0.05),脂肪组织睾酮、雌二醇、芳香化酶、TNF-R1高于N组(P<0.05),血清睾酮低于N组(P<0.05);FO组体重、体脂百分比、进食量、摄入热量、Lee’s指数和血清雌二醇高于N组(P<0.01)。(2)经过8周有氧运动后,FE组体重低于FO组,脂肪组织睾酮、雌二醇、IL6、TNFα和血清雌二醇,脂肪组织芳香化酶和TNF-R1蛋白表达低于FO组(P<0.05);FE组进食量、摄入热量、腹腔脂肪重量、体脂百分比低于FO组(P<0.01)。(3)经过8周饮食干预后,FN组Lee’s指数,血清雌二醇,脂肪组织TNF-R1蛋白表达低于FO组(P<0.05);FN组体重、进食量、摄入热量、腹腔脂肪重量,体脂百分比,脂肪组织睾酮、雌二醇、IL6和TNFα低于FO组(P<0.01)。结论:(1)高脂饮食诱发肥胖会引起雄性小鼠脂肪组织炎症因子和芳香化酶表达增加,进而引起机体雌激素水平升高。(2)有氧运动可能主要通过TNFα-TNFR1途径降低了雄性肥胖小鼠脂肪组织芳香化酶的表达,并且有助于脂肪组织雄激素芳香化作用恢复平衡。(3)降脂饮食干预可能并不是主要通过脂肪组织炎症-芳香化酶途径影响雄性肥胖小鼠的性激素水平。 | 马铁 赵大林 李涛 衣雪洁 | 2019 | 中国运动医学杂志2019，38，7： | 2 |
| 2 | Inhibitory Effect of Estrogens,Phytoestrogens,and Caloric Restriction on Oxidative Stress and Hepato-toxicity in Aged Rats显示文摘Objective To investigate the protective effect of 17β-estradiol (E2), peganum harmala extract (PHE) administration and calorie restriction (CR) treatment (60%) on oxidative stress and hepato-toxicity in aged rats. Methods Eighteen months old animals that were treated at the age of 12 months were divided into 4 groups: normal control group with free access to food, E2 treatment group, PHE treatment group and CR treatment group of the food given to control group. Six male rats at the age of 4 months were used as a reference group. Results Aging significantly decreased superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), and increased lactate deshydrogenase (LDH), gamma-glytamyl transferase (GGT), phosphatase alkalines (PAL), aspartate and lactate transaminase (AST and ALT) activities in the liver. Aging also induced an increased lipid peroxidation level, histological changes and a decreased E2 level. However, treatment with E2, PHE, and CR increased 17β-estradiol, and decreased hepatic dysfunction parameters and lipid peroxidation as well as histological changes in the liver of aged rats. Conclusion The antioxidant and hepatoprotective activity of PHE and CR is possibly attributed to its ability to increase E2 level, which as an antioxidant, acts as a scavenger of ROS. Further studies on the pharmaceutical functions of E2 in males may contribute to its clinical application. | KHALED HAMDEN SERGE CARREAU FATMA AYADI HATEM MASMOUDI ABDELFATTAH EL FEKI | 2009 | Biomedical and Environmental Sciences2009，22，5： | 1 |
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