| 1 | Connexin43介导胃肠神经-Cajal间质细胞-平滑肌网络缝隙连接通讯的研究进展显示文摘缝隙连接蛋白(Connexin,Cx)广泛分布于各器官、组织中(红细胞、骨骼肌除外),目前发现的Cx至少有20种~[1],是构成缝隙连接(Gap junction,GJ)的基本结构和功能蛋白,其中缝隙连接蛋白43(Connexin43,Cx43)的表达最广泛~[2],迄今已发现存在于34种组织和46种细胞中~[3]。Cx43介导的缝隙连接通迅(gap junction intercellular communication,GJIC) | 禹振华 黄忠诚 | 2015 | 结直肠肛门外科2015，21，6： | 3 |
| 2 | Deletion of Gpr128 results in weight loss and increased intestinal contraction frequency显示文摘AIM:To generate a Gpr128 gene knockout mouse model and to investigate its phenotypes and the biological function of the Gpr128 gene.METHODS:Bacterial artificial chromosome-retrieval methods were used for constructing the targeting vector.Using homologous recombination and microinjection technology,a Gpr128 knockout mouse model on a mixed 129/BL6 background was generated.The mice were genotyped by polymerase chain reaction(PCR)analysis of tail DNA and fed a standard laboratory chow diet.Animals of both sexes were used,and the phenotypes were assessed by histological,biochemical,molecular and physiological analyses.Semi-quantitative reverse transcription-PCR and Northern blotting were used to determine the tissue distribution of Gpr128mRNA.Beginning at the age of 4 wk,body weights were recorded every 4 wk.Food,feces,blood and organ samples were collected to analyze food consumption,fecal quantity,organ weight and constituents of the blood and plasma.A Trendelenburg preparation was utilized to examine intestinal motility in wild-type(WT)and Gpr128-/-mice at the age of 8 and 32 wk.RESULTS:Gpr128 mRNA was highly and exclusively detected in the intestinal tissues.Targeted deletion of Gpr128 in adult mice resulted in reduced body weight gain,and mutant mice exhibited an increased frequency of peristaltic contraction and slow wave potential of the small intestine.The Gpr128+/+mice gained more weight on average than the Gpr128-/-mice since 24 wk,being 30.81±2.84 g and 25.74±4.50 g,respectively(n=10,P<0.01).The frequency of small intestinal peristaltic contraction was increased in Gpr128-/-mice.At the age of 8 wk,the frequency of peristalsis with an intraluminal pressure of 3 cmH2O was 6.6±2.3 peristalsis/15 min in Gpr128-/-intestine(n=5)vs 2.6±1.7peristalsis/15 min in WT intestine(n=5,P<0.05).At the age of 32 wk,the frequency of peristaltic contraction with an intraluminal pressure of 2 and 3 cmH2O was 4.6±2.3 and 3.1±0.8 peristalsis/15 min in WT mice(n=8),whereas in Gpr128-/-mice(n=8)the frequency of contraction was 8.3±3.0 and 7.4±3.1peristalsis/15 min,respectively(2 cmH2O:P<0.05 vs WT;3 cmH2O:P<0.01 vs WT).The frequency of slow wave potential in Gpr128-/-intestine(35.8±4.3,36.4±4.2 and 37.1±4.8/min with an intraluminal pressure of 1,2 and 3 cmH2O,n=8)was also higher than in WT intestine(30.6±4.2,31.4±3.9 and 31.9±4.5/min,n=8,P<0.05).CONCLUSION:We have generated a mouse model with a targeted deletion of Gpr128 and found reduced body weight and increased intestinal contraction frequency in this animal model. | Ying-Yin Ni Yan Chen Shun-Yuan Lu Bi-Ying Sun Fang Wang Xiao-Lin Wu Su-Ying Dang Guo-Hua Zhang Hong-Xin Zhang Yin Kuang Jian Fei Ming-Min Gu Wei-Fang Rong Zhu-Gang Wang | 2014 | World Journal of Gastroenterology2014，20，2： | 0 |
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