共被期刊论文引用了2次
您的检索式：您选中1篇文献正在查看引证文献汇总
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Distribution of Prestin on Outer Hair Cell Basolateral Surface显示文摘Prestin has been identified as a motor protein responsible for outer hair cell (OHC) electromotility and is expressed on the OHC surface. Previous studies revealed that OHC electromotility and its associated nonlinear capacitance were mainly located at the OHC lateral wall and absent at the apical cuticular plate and the basal nucleus region. Immunofluorescent staining for prestin also failed to demonstrate prestin expression at the OHC basal ends in whole-mount preparation of the organ of Corti. However, there lacks a definitive demonstration of the pattern of prestin distribution. The OHC lateral wall has a trilaminate organization and is composed of the plasma membrane, cortical lattice, and subsurface cisternae. In this study, the location of prestin proteins in dissociated OHCs was examined using immunofluorescent staining and confocal microscopy. We found that prestin was uniformly expressed on the basolateral surface, including the basal pole. No staining was seen on the cuticular plate and stereocilia. When co-stained with a membrane marker di-8-ANEPPS, prestin-labeling was found to be in the outer layer of the OHC lateral wall. After separating the plasma membrane from the underlying subsurface cisternae using a hypotonic extracellular solution, prestin-labeling was found to be in the plasma membrane, not the subsurface cisternae. The data show that prestin is expressed in the plasma membrane on the entire OHC basolateral surface. | YU Ning1,2, ZHAI Suo-qiang1, YANG Shi-ming1, HAN Dong-yi1, ZHAO Hong-bo2, 1 Department of Otolaryngology & Institute of Otolaryngology, CPLA General Hospital, BJ, 100853 2 Department of Surgery-Otolaryngology, University of Kentucky Medical Center, Lexington, KY 40536-0293 | 2008 | Journal of Otology2008，3，2： | 3 |
| 2 | An Overview of Electrically Evoked Otoacoustic Emissions in the Mammalian Cochlea显示文摘Alternating currents injected into the cochlea are able to evoke outer hair cell-mediated basilar membrane motion, thus give rise to production of otoacoustic emissions. This electrically evoked otoacoustic emission(EEOAE) provides a useful tool for the research of out hair cell electromotility in vivo. This article reviews the research work on EEOAEs in mammals. Features of the EEOAEs and theories of their generation are introduced. Methods of EEOAE measurement are also described. | Alfred L. Nuttall | 2006 | Journal of Otology2006，1，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员