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| 1 | 太湖不同湖区真核微型浮游生物基因多样性的研究显示文摘用DGGE(denatured gradient gel electrophoresis)和构建18SrDNA克隆文库2种方法对太湖不同湖区的真核微型浮游生物(0.8-20μm)多样性及组成结构进行了研究.DGGE结果表明,不同湖区真核微型浮游生物的DGGE指纹图谱存在明显差异,其中营养水平较低的东太湖和贡湖DGGE条带数最多,分别为23和24,香农多样性指数分别为3.135和3.178,而营养水平较高的梅梁湾和五里湖最少,均为18,香农多样性指数为2.890,表明营养水平较低湖区的多样性高于营养水平较高的湖区.克隆测序结果表明太湖中真核微型浮游生物种类丰富,占优势的主要是一些鞭毛藻、异养鞭毛虫、纤毛虫和真菌,而营养水平不同的梅梁湾、湖心、东太湖中真核微型浮游生物组成明显不同.在营养水平较高的梅梁湾,28.6%的OUT(operational taxonomicunit)属于异养鞭毛虫,另外隐藻、金藻分别占22.9%和14.3%;在湖心,金藻的比例最大,占25.7%,另外比较多的是异养鞭毛虫和隐藻,分别为20.0%和14.3%;而营养水平较低的东太湖各类纤毛虫所占比份最大,为26.8%,异养鞭毛虫较少,仅占4.9%,另外真菌含量较高,占12.2%. | 陈美军 孔繁翔 陈非洲 邢鹏 | 2008 | 环境科学2008，29，3： | 24 |
| 2 | Phylogenetic diversity and specificity of bacteria associated with Microcystis aeruginosa and other cyanobacteria显示文摘Interactions between bacteria and cyanobacteria have been suggested to have a potential to influence harmful algal bloom dynamics; however, little information on these interactions has been reported. In this study, the bacterial communities associated with five strains of Microcystis aeruginosa, three species of other Microcystis spp., and four representative species of non-Microcystis cyanobacteria were compared. Bacterial 16S rDNA fragments were amplified and separated by denaturing gradient gel electrophoresis (DGGE) followed by DNA sequence analysis. The similarities among bacterial communities associated with these cyanobacteria were compared to the digitized DGGE profiles using the cluster analyses. The bacterial community structure of all cyanobacterial cultures differed. Cluster analysis showed that the similarity values among M. aeruginosa cultures were higher than those of other cyanobacterial cultures. Sequence analysis of DGGE fragments indicated the presence of bacteria including, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Bacteroidetes and Actinobacteria in the cyanobacterial cultures. Members of the Sphingomonadales were the prevalent group among the Microcystis-associated bacteria. The results provided further evidence for species-specific associations between cyanoabcteria and heterotrophic bacteria, which are useful for understanding interactions between Microcystis and their associated bacteria. | SHI Limei CAI Yuanfeng YANG Hualin XING Peng LI Pengfu KONG Lingdong KONG Fanxiang | 2009 | Journal of Environmental Sciences2009，21，11： | 11 |
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