|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 烟草青枯病发病烟株根际土壤细菌群落分析显示文摘【目的】研究青枯病发生对根际土壤细菌群落组成的影响,探明影响根际土壤细菌群落的环境因子。【方法】采用宏基因组学高通量测序技术,比较发病烟株和健康烟株根际土壤细菌群落差异。【结果】(1)健康烟株根际土壤细菌Ace指数、Chao指数和Shannon指数有高于发病烟株的趋势,而Simpson指数有低于发病烟株的趋势;(2)发病烟株根际土壤中芽孢杆菌属(Bacillus)、假单胞菌属(Pseudomonas)和链霉菌属(Streptomyces)等益生菌减少,而劳尔氏菌属(Ralstonia)和Rudaea等病原菌增加;(3)土壤pH、有效磷(AP)、交换性钙(Ca)和有效铜(Cu)含量在发病烟株根际土壤中显著减少,而速效钾(AK)含量显著增加。【结论】与健康烟株根际土壤相比,发病烟株根际土壤细菌多样性、有益菌丰度、土壤pH和养分含量都较低;pH、P、K、Ca、Cu是对根际土壤细菌群落影响较大的土壤环境因子。 | 施河丽 向必坤 谭军 彭五星 孙玉晓 王瑞 吴文昊 魏国胜 丁才夫 | 2018 | 中国烟草学报2018，24，5： | 34 |
| 2 | 钙镁磷肥对青枯病发病烟株根际土壤微生物区系的影响显示文摘采用平板计数法分析了不同生育期及发病烤烟根际可培养微生物数量的变化,并通过宏基因组测序法比较了施用钙镁磷肥的健康与发病烟株根际土壤微生物群落的差异,旨在研究钙镁磷肥对烟株根际土壤微生物群落多样性的影响。结果表明:1)施用钙镁磷肥的烤烟根际细菌和真菌数量随生育进程呈抛物线形式变化,对照则呈'N'趋势,处理和对照的根际放线菌数量变化趋势相同,呈逐渐增加的趋势。2)在旺长期,施用钙镁磷肥的根际细菌数量显著高于对照;在成熟期,处理的根际真菌数量显著高于对照;而钙镁磷肥对根际放线菌数量没有显著影响。3)相对于健康烟株根际土壤,发病烟株根际土壤中放线菌数量显著减少,细菌数量显著增加;发病根际土壤中施用钙镁磷肥的细菌和放线菌数量均显著高于对照。4)宏基因组测序结果显示根际细菌种类总数远高于根际真菌,健康根际细菌和真菌香农指数、Chao1指数和ACE指数均高于发病根际土壤。综上所述,钙镁磷肥对根际土壤微生物数量和多样性有一定的促进作用。 | 李小龙 李红丽 曾强 郭夏丽 王岩 | 2016 | 中国烟草学报2016，22，1： | 12 |
| 3 | 烟草青枯病发病土壤理化性状及细菌群落结构分析显示文摘为了解土壤理化性状及细菌群落结构与青枯病发生的关系,在湖北恩施典型烟区采集了160个植烟土壤(82个未发病土壤,78个发病土壤),检测了土壤理化指标,并采用16S rRNA基因测序技术分析了烟株发病与未发病根际土壤细菌群落结构的差异。结果表明:(1)与未发病土壤相比,发病土壤细菌群落结构多样性更高。(2)发病土壤劳尔氏菌属(Ralstonia solanacearum)相对丰度显著高于未发病土壤,而鞘氨醇单胞菌属(Sphingomonas)、节杆菌属(Arthrobacter)和假单胞菌属(Pseudomonas)相对丰度则相反。(3)青枯病的发生使土壤微生物网络结构更复杂而稳定性降低,并改变了部分核心点位细菌种类。(4)典范对应分析(CCA)表明,土壤pH和速效磷(AP)可能是影响烟草青枯病发生的重要土壤因子。综上所述,土壤pH、AP与细菌Sphingomonas、Arthrobacter、Pseudomonas相对丰度的增加和Ralstonia相对丰度的降低可能是减少烟田青枯病发病的关键因素。 | 樊俊 谭军 王瑞 邓建强 张继光 徐大兵 施河丽 向必坤 | 2021 | 中国烟草科学2021，42，6： | 10 |
| 4 | 烟草青枯病发病程度的影响因素分析显示文摘为明确影响烟草青枯病发生的关键土壤因子,在青枯病发生的典型烟区采集了24个不同发病程度的植烟土壤样品,并采用分组比较、典范对应分析、主成分分析和最小数据集等方法研究了不同发病程度的土壤理化性状与细菌群落结构间的差异。结果表明,随着青枯病病情指数的提高,土壤pH、交换性钙(ECa)、有效锰(AMn)和通气孔隙度(SAP)呈显著降低的趋势,土壤含水率(SWC)和毛管持水量(SCC)呈明显增加趋势。烟草青枯病的发生对植烟土壤主要门水平细菌相对丰度影响较小,对鞘氨醇单胞菌属(Sphingomonas)、土壤红杆菌属(Solirubrobacter)、根瘤菌属(Rhizobium)的OTUs数量有显著降低效果。综合分析得出,SCC、pH、AMn和鞘氨醇单胞菌属、根瘤菌属的OTUs数量与病情指数的相关性达到显著(P<0.05)水平,是影响烟草青枯病发生的关键因子。 | 樊俊 谭军 王瑞 施河丽 夏鹏亮 赵秀云 | 2021 | 烟草科技2021，54，10： | 7 |
| 5 | 雪茄烟田微生物群落和土壤理化性状与青枯病发生的关系显示文摘为明确影响雪茄烟青枯病发生的关键微生物和土壤因素,采用扩增子测序法,研究了青枯病发病与未发病根际土壤细菌和真菌群落结构组成、土壤理化性状及相互间的关系。结果表明,相对于未发病根际土壤,发病土壤有提高微生物多样性和复杂程度的趋势,增加了假单胞菌(Pseudomonas)、肠杆菌(Enterobacter)、弹球菌(Sphaerobolus)、毛枝菌(Trichocladium)、镰刀菌(Fusarium)等的相对丰度,降低了朱氏杆菌(Chujaibacter)、寡养单胞菌(Stenotrophomonas)、罗河杆菌(Rhodanobacter)、被孢霉菌(Mortierella)、毛壳菌(Chaetomium)等的相对丰度。病情指数与土壤含水量(SAWC)、毛管孔隙度(SCM)、有效磷(AP)显著正相关;与土壤通气孔隙度(SAP)、p H、交换性钙(ECa)显著负相关。典型相关、主成分及最小数据集分析表明土壤SCM、AP和劳尔氏菌(Ralstonia)、Chujaibacter相对丰度是雪茄烟青枯病发病的关键土壤及微生物因子。 | 樊俊 向必坤 谭军 王瑞 谭绍安 肖亮 王雪松 | 2022 | 中国烟草科学2022，43，5： | 2 |
| 6 | Conidia of one Fusarium solani isolate from a soybean-production field enable to be virulent to soybean and make soybean seedlings wilted显示文摘Fusarium is usually thought to cause soybean root rot,which results in a large quantity of annual yield loss in soybean production,by its secretions including Fusarium toxins and cell wall degrading enzymes,but not by the conidia themselves that do not underlie any virulence so far.Here we report that the conidia of one Fusarium solani isolate are able to be virulent to soybean and make soybean seedlings wilted alone.We isolated them from the wilted plants in a soybean-production field and molecularly identified 17 Fusarium isolates through phylogenetic analysis.Of them,except for one isolate that showed diversity of virulence to different soybeans(virulent to one soybean whereas avirulent to another soybean),the others were all virulent to the two tested soybeans:both conidia cultures and secretions could make soybean seedlings wilted at 5 days post infection,and their virulence had dosage effects that only conidia cultures of at least 5×10~6 conidia mL^(-1) could show virulence to soybean;however,the sole conidia of the F.solani isolate #4 also exhibited virulence to soybean and could make soybean seedlings wilted.Finally,we developed the specific cleaved amplified polymorphic sequences(CAPS)markers to easily differentiate Fusarium isolates.The isolate #4 in this work will likely be used to investigate the new mechanism of virulence of Fusarium to soybean. | ZHENG Na ZHANG Liu-ping GE Feng-yong HUANG Wen-kun KONG Ling-an PENG De-liang LIU Shi-ming | 2018 | Journal of Integrative Agriculture2018，17，9： | 1 |
| 7 | Frequent Applications of Organic Matter to Agricultural Soil Increase Fungistasis显示文摘Soil-borne plant pathogens are among the most important limiting factors for the productivity of agro-ecosystems.Fungistasis is the natural capability of soils to inhibit the germination and growth of soil-borne fungi in the presence of optimal abiotic conditions.The objective of this study was to assess the effects of different soil managements,in terms of soil amendment types and frequency of application,on fungistasis.For this purpose,a microcosm experiment was performed by conditioning a soil with frequent applications of organic matter with contrasting biochemical quality(i.e.,glucose,alfalfa straw and wheat straw).Thereafter,the fungistasis response was assessed on four fungi(Aspergillus niger,Botrytis cinerea,Pyrenochaeta lycopersici and Trichoderma harzianum).Conditioned soils were characterized by measuring microbial activity(soil respiration) and functional diversity using the BIOLOG EcoPlates^(?)method.Results showed that irrespective of the fungal species and amendment types,frequent applications of organic matter reduced fungistasis relief and shortened the time required for fungistasis restoration.The frequent addition of easily decomposable organic compounds enhanced soil respiration and its specific catabolic capabilities.This study demonstrated that frequent applications of organic matter affected soil fungistasis likely as a result of higher microbial activity and functional diversity. | Giuliano BONANOMI Salvatore A. GAGLIONE Gaspare CESARANO Tushar C. SARKER Marica PASCALE Felice SCALA Astolfo ZOINA | 2017 | Pedosphere2017，27，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员