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| 1 | A Pilot Study of the Therapeutic Efficacy and Mechanism of Artesunate in the MRL/lpr Murine Model of Systemic Lupus Erythematosus显示文摘Recent evidence indicates that artesunate has immunomodulatory properties that might be useful for treating autoimmune disease.In this study,we conducted a pilot study and explored the effect and mechanism of artesunate on the treatment of systemic lupus erythematosus using an MRL/lpr murine model.MRL/lpr mice were divided into control,cyclophosphamide(CTX)and artesunate treatment groups.Blood was collected to measure serum levels of creatinine,antinuclear antibody(ANA)and anti-double-stranded DNA(anti-dsDNA)antibody.Twenty-four-hour urine was collected to measure levels of proteinuria.The concentration of monocyte chemotactic protein-1(MCP-1)in serum and urine was measured.The expression of MCP-1 in kidney was detected by Western blot and immunohistochemistry assay,respectively.The expression of B cell activating factor(BAFF)in spleen was determined by real time-PCR and immunoblotting.We found that artesunate significantly increased the survival rate,body weight and blood leukocyte counts,and reduced the serum levels of ANA and anti-dsDNA antibody titer,24 h urinary protein,and serum creatinine.Our results indicated that artesunate could decrease MCP-1,major pro-inflammation cytokine,in serum,urine and kidney.We also found that the level of BAFF,the major B cell activation factor,was decreased in artesunate treated MRL/lpr mice.Its efficacy was comparable with that of CTX in this study.Taken together,we have demonstrated that artesunate can inhibit the progression of disease and reverse the pathologic lesion of lupus nephritis. | Ouyang Jin Huayong Zhang Zhifeng Gu Shengnan Zhao Ting Xu Kangxing Zhou Bo Jiang Jie Wang Xiaofeng Zeng Lingyun Sun | 2009 | Cellular & Molecular Immunology2009，6，6： | 20 |
| 2 | Multi-block SSD based on small object detection for UAV railway scene surveillance显示文摘A method of multi-block Single Shot Multi Box Detector(SSD)based on small object detection is proposed to the railway scene of unmanned aerial vehicle surveillance.To address the limitation of small object detection,a multi-block SSD mechanism,which consists of three steps,is designed.First,the original input images are segmented into several overlapped patches.Second,each patch is separately fed into an SSD to detect the objects.Third,the patches are merged together through two stages.In the first stage,the truncated object of the sub-layer detection result is spliced.In the second stage,a sub-layer suppression and filtering algorithm applying the concept of non-maximum suppression is utilized to remove the overlapped boxes of sub-layers.The boxes that are not detected in the main-layer are retained.In addition,no sufficient labeled training samples of railway circumstance are available,thereby hindering the deployment of SSD.A two-stage training strategy leveraging to transfer learning is adopted to solve this issue.The deep learning model is preliminarily trained using labeled data of numerous auxiliaries,and then it is refined using only a few samples of railway scene.A railway spot in China,which is easily damaged by landslides,is investigated as a case study.Experimental results show that the proposed multi-block SSD method produces an overall accuracy of 96.6%and obtains an improvement of up to 9.2%compared with the traditional SSD. | Yundong LI Han DONG Hongguang LI Xueyan ZHANG Baochang ZHANG Zhifeng XIAO | 2020 | Chinese Journal of Aeronautics2020，33，6： | 19 |
| 3 | One-year clinical study of NeuroR egen scaffold implantation following scar resection in complete chronic spinal cord injury patients显示文摘The objective of this clinical study was to assess the safety and feasibility of the collagen scaffold, Neuro Regen scaffold, one year after scar tissue resection and implantation. Scar tissue is a physical and chemical barrier that prevents neural regeneration. However, identification of scar tissue is still a major challenge. In this study, the nerve electrophysiology method was used to distinguish scar tissue from normal neural tissue, and then different lengths of scars ranging from 0.5–4.5 cm were surgically resected in five complete chronic spinal cord injury(SCI) patients. The NeuroR egen scaffold along with autologous bone marrow mononuclear cells(BMMCs), which have been proven to promote neural regeneration and SCI recovery in animal models, were transplanted into the gap in the spinal cord following scar tissue resection. No obvious adverse effects related to scar resection or Neuro Regen scaffold transplantation were observed immediately after surgery or at the 12-month follow-up. In addition, patients showed partially autonomic nervous function improvement, and the recovery of somatosensory evoked potentials(SSEP) from the lower limbs was also detected. The results indicate that scar resection and Neuro Regen scaffold transplantation could be a promising clinical approach to treating SCI. | Zhifeng Xiao Fengwu Tang Jiaguang Tang Huilin Yang Yannan Zhao Bing Chen Sufang Han Nuo Wang Xing Li Shixiang Cheng Guang Han Changyu Zhao Xiaoxiong Yang Yumei Chen Qin Shi Shuxun Hou Sai Zhang Jianwu Dai | 2016 | Science China(Life Sciences)2016，59，7： | 18 |
| 4 | Focal depths for moderate-sized aftershocks of the Wenchuan M_S8.0 earthquake and their implications显示文摘Sliding-window cross-correlation method is firstly adopted to identify sPn phase, and to constrain focal depth from regional seismograms, by measuring the time separation between sPn and Pn phases. We present the focal depths of the 17 moderate-sized aftershocks (MS≥5.0) of the Wenchuan MS8.0 earth-quake, using the data recorded by the regional seismic broadband networks of Shaanxi, Qinghai, Gansu, Yunnan and Sichuan. Our results show focal depths of aftershocks range from 8 to 20 km, and tend to cluster at two average depths, separate at 32.5°N, i.e., 11 km to the south and 17 km to the north, indicating that these aftershocks are origin of upper-to-middle crust. Combined with other results, we suggest that the Longmenshan fault is not a through-going crustal fault and the Pingwu-Qingchuan fault may be not the northward extension of the Longmenshan thrust fault. | ZHANG RuiQing WU QingJu LI YongHua DING ZhiFeng ZENG RongSheng | 2008 | Science China Earth Sciences2008，51，12： | 14 |
| 5 | Xuebijing injection alleviates liver injury by inhibiting secretory function of Kupffer cells in heat stroke rats显示文摘OBJECTIVE:To evaluate the effects of Xuebijing(XBJ) injection in heat stroke(HS) rats and to investigate the mechanisms underlying these effects.METHODS:Sixty anesthetized rats were randomized into three groups and intravenously injected twice daily for 3 days with 4 mL XBJ(XBJ group) or phosphate buffered saline(HS and Sham groups) per kg body weight.HS was initiated in the HS and XBJ groups by placing rats in a simulated climate chamber(ambient temperature 40 ℃,humidity 60%).Rectal temperature,aterial pressure,and heart rate were monitored and recorded.Time to HS onset and survival were determined,and serum concentrations of tumor necrosis factor(TNF)-α,inRESULTS:Time to HS onset and survival were significantly longer in the XBJ than in the HS group.Moreover,the concentrations of TNF-α,IL-1β,IL-6,ALT and AST were lower and liver injury was milder in the XBJ than in the HS group.Heat-stress induced structural changes in KCs and hepatic cells were more severe in the HS than in the XBJ group and the concentrations of TNF-α,IL-β and IL-6 secreted by KCs were lower in the XBJ than in the HS group.CONCLUSION:XBJ can alleviate HS-induced systemic inflammatory response syndrome and liver injury in rats,and improve outcomes.These protective effects may be due to the ability of XBJ to inhibit cytokine secretion by KCs. | Yi Chen Huasheng Tong Xingqin Zhang Liqun Tang Zhiguo Pan Zhifeng Liu Pengkai Duan Lei Su | 2013 | Journal of Traditional Chinese Medicine2013，33，2： | 14 |
| 6 | Impact of anthropogenic activities on the sediment microbial communities of Baiyangdian shallow lake显示文摘Microorganisms are recognized as sensors of external disturbance in freshwater ecosystems.In this study,sediment samples from regions with strong human activity interference(four sites),moderate human activity interference(five sites)and minor human activity interference(five sites)in Baiyangdian Lake were collected;and their physiological and chemical properties and heavy metals contents were analyzed.The structures of bacterial and fungal communities were tested using 16S ribosomal Ribonucleic Acid(rRNA)and Internal Transcribed Spacer 2(ITS2)high-throughput sequencing technologies.The results indicated that Proteobacteria was the most abundant bacterial phylum.Ascomycota,Basidiomycota and Ciliophora were the dominant phyla of fungi.Obvious differences of microbial genera among the three regions were observed,especially fungal genera.With intensified disturbance by human activities,the Simpson and Shannon indices of bacteria significantly decreased.The fungal communities were clustered into three groups in accordance with the different human disturbance levels.The bacterial communities of sediment samples under moderate and minor human disturbance appeared similar,but different from those under strong human disturbance.Redundancy analysis indicated that zinc,total phosphorus,copper,and lead were the dominant factors that influenced the bacterial community.Nitrifiers and sulfate-reducing bacteria were influenced by pH,nitrate(NO3 ),and total nitrogen.The fungal community was significantly affected by total phosphorus.This study reveals that sediment microbial community structures among different regions of a shallow lake are mainly impacted by human activities. | Wenjun Wang Yujun Yi Yufeng Yang Yang Zhou Shanghong Zhang Xuan Wang Zhifeng Yang | 2020 | International Journal of Sediment Research2020，35，2： | 14 |
| 7 | Study on Multiple Electromagnetic Continuous Casting of Aluminum Alloy显示文摘与高质量获得半固体艾尔合金营舍,调查被在连续的扔从一个铜模子的外面强加一个多重磁场执行。为处理的半固体金属(SSM ) 设计的 AISi6Mg2 合金连续地在各种各样的条件下面通过一张沉没入口嘴被扔。新月运动,温度分发和营舍质量上的多重磁场的效果被检验。试验性的结果证明那新月骚乱引起了由电磁激动人心能有效地被控制,半固体艾尔合金营舍的表面质量极大地被改进,并且越过营舍的横向的节的一致地好的、球状的微观结构被优化多重磁场的分发完成。 | Zhifeng ZHANG Jun XUN Likai SHI | 2006 | Journal of Materials Science & Technology2006，22，4： | 13 |
| 8 | Numerical simulation on electromagnetic field,flow field and temperature field in semisolid slurry preparation by A-EMS显示文摘A two-dimensional computational model coupling an annular electromagnetic stirring(A-EMS) with a macroscopic heat and fluid flow analysis in Al-alloys semisolid slurry preparation was developed.The dynamic evolutions of the electromagnetic field,flow field,and temperature field were presented successfully by commercial software ANSYS 10.0 with corresponding experimental verification.A horizontally rotational electromagnetic field and,thereby,a more intensive velocity field were uniformly distributed in the stirred melt even at commercial frequency,and thus,a lower temperature difference in the stirred melt and subsequent uniformly fine microstructures were obtained compared with the normal electromagnetic stirring.The simulation results were in good agreement with experimental ones. | ZHANG Zhifeng,CHEN Xingrun,XU Jun,and SHI Likai National Engineering & Technology Research Center for Nonferrous Metal Matrix Composites,General Research Institute for Nonferrous Metals,Beijing 100088,China | 2010 | Rare Metals2010，29，6： | 13 |
| 9 | Water regime-nitrogen fertilizer incorporation interaction:Field study on methane and nitrous oxide emissions from a rice agroecosystem in Harbin, China显示文摘Water regime and nitrogen(N) fertilizer are two important factors impacting greenhouse gases(GHG) emission from paddy field, whereas their effects have not been well studied in cold region. In this study, we conducted a two-year field experiment to study the impacts of water regime and N fertilizer on rice yields and GHG emissions in Harbin, China, a cold region located in high latitudes. Our results showed that intermittent irrigation significantly decreased methane(CH_4) emission compared with continuous flooding, however,the decrement was far lower than the global average level. The N_2O emissions were very small when flooded but peaked at the beginning of the disappearance of floodwater. The N fertilizer treatments increased CH_4 emissions at low level(75 kg N/ha). But both CH_4 and N_2O emissions were uninfluenced at the levels of 150 kg N/ha and 225 kg N/ha. Rice yields increased under intermittent irrigation and were highest at the level of 150 kg N/ha. From our results, we recommended that the intermittent irrigation and 150 kg N/ha as the ideal water regime-nitrogen fertilizer incorporation for this area to achieve low GHG emissions without impacting rice yields. | Wenjun Dong Jia Guo Lijun Xu Zhifeng Song Jun Zhang Ao Tang Xijuan Zhang Chunxu Leng Youhong Liu Lianmin Wang Lizhi Wang Yang Yu Zhongliang Yang Yilei Yu Ying Meng Yongcai Lai | 2018 | Journal of Environmental Sciences2018，30，2： | 12 |
| 10 | Annular electromagnetic stirring——a new method for the production of semi-solid A357 aluminum alloy slurry显示文摘A new method for producing semisolid slurry,annular electromagnetic stirring(AEMS),to refine and spheroidize grains was exploited.Experimental work was undertaken to investigate the effects of cooling rate,stirring power and stirring time on the solidification behavior of A357 alloy using A-EMS.It was found that increasing the cooling rate and stirring power gave rise to substantial grain refinement,which could be attributed to the increase of effective nucleation rate caused by the extremely uniform temperature and composition fields in the bulk liquid during the initial stage of solidification.Results showed that a fully grain refined spherical structure could be obtained using proper processing conditions within 10s. | Guanglei ZHU Jun XU Zhifeng ZHANG Yuelong BAI Likai SHI | 2009 | Acta Metallurgica Sinica(English Letters)2009，22，6： | 11 |
| 11 | The Effect of Toxic Sulfide Exposure on Oxygen Consumption and Oxidation Products in Urechis unicinctus(Echiura:Urechidae)显示文摘To investigate sulfide detoxification in Urechis unicinctus,oxygen consumption rate and sulfide detoxification productswere analyzed during sulfide exposure under controlled laboratory conditions.The results showed that oxygen consumption rateswere elevated significantly during 3 h sulfide exposure compared to the control(P<0.05).The concentration of sulfite in body walland hindgut of experimental worms increased significantly(P<0.05)when exposed to 50μmolL-1sulfide,reached a maximum at24 h and then decreased.Similar result was observed in worms exposed to 150μmolL-1sulfide except that sulfite concentrationreached a maximum at 12 h.Contents of thiosulfate in body wall and hindgut of U.unicinctus exposed to sulfide were also significantly higher than that of the control without sulfide exposure.In conclusion,during short-time sulfide exposure U.unicinctus mayraise oxygen consumption to detoxify toxic sulfide into sulfite and thiosulfate.Sulfide detoxification was restrained when the expo-sure time was prolonged or sulfide concentration was increased,which was indicated by decrease of sulfite,the intermediate productof sulfide detoxification. | WANG Sifeng ZHANG Zhifeng CUI He KANG Kyoung Ho MA Zhuojun | 2010 | Journal of Ocean University of China2010，9，2： | 10 |
| 12 | Tomographic imaging of P wave velocity structure beneath the region around Beijing显示文摘The three-dimension crustal and upper mantle structures in the region around Beijing were studied by seismic tomography. We used the P wave arrival times from local and teleseismic events. These events were recorded by 250 stations of the North China Seismic Array and 108 stations of the Beijing Telemetry Seismic Network. 118 869 P wave arrivals from 10 285 local events and 12 189 P wave arrivals from 107 teleseismic events were used in the inversion. We obtained the 3-D P wave velocity structure of the crust and upper mantle with the horizontal resolution of 0.3° in the studied region. The tomographic imaging shows the remarkably heterogeneous velocity variation. The velocity anomalies are in well agreement with the geological structure in the shallow crust. The different relationships between seismic activities and velocity anomalies may imply the different seismogenic structure and mechanism. Beneath the Moho under Taihangshan mountain and Yanshan mountain,we found the high velocity anomalies deep to 120 km and 200 km,respectively. The deep high velocity zone may be explained by the existence of the mountain root under Yanshan mountain. The high velocity anomalies in the upper mantle of North China basin may be the relics of the de-rooting from the former craton mantle lithosphere. | Zhifeng Ding Xiaofeng Zhou Yan Wu Guiyin Li Hong Zhang | 2009 | Earthquake Science2009，22，4： | 10 |
| 13 | Cytocompatibility with osteogenic cells and enhanced in vivo anti-infection potential of quaternized chitosan-loaded titania nanotubes显示文摘Infection is one of the major causes of failure of orthopedic implants. Our previous study demonstrated that nanotube modification of the implant surface, together with nanotubes loaded with quaternized chitosan(hydroxypropyltrimethyl ammonium chloride chitosan, HACC), could effectively inhibit bacterial adherence and biofilm formation in vitro. Therefore, the aim of this study was to further investigate the in vitro cytocompatibility with osteogenic cells and the in vivo anti-infection activity of titanium implants with HACC-loaded nanotubes(NT-H). The titanium implant(Ti), nanotubes without polymer loading(NT), and nanotubes loaded with chitosan(NT-C) were fabricated and served as controls. Firstly, we evaluated the cytocompatibility of these specimens with human bone marrow-derived mesenchymal stem cells in vitro.The observation of cell attachment, proliferation, spreading, and viability in vitro showed that NT-H has improved osteogenic activity compared with Ti and NT-C. A prophylaxis rat model with implantation in the femoral medullary cavity and inoculation with methicillin-resistant Staphylococcus aureus was established and evaluated by radiographical, microbiological, and histopathological assessments. Our in vivo study demonstrated that NT-H coatings exhibited significant anti-infection capability compared with the Ti and NT-C groups. In conclusion, HACC-loaded nanotubes fabricated on a titanium substrate show good compatibility with osteogenic cells and enhanced anti-infection ability in vivo, providing a good foundation for clinical application to combat orthopedic implant-associated infections. | Ying Yang Haiyong Ao Yugang Wang Wentao Lin Shengbing Yang Shuhong Zhang Zhifeng Yu Tingting Tang | 2016 | Bone Research2016，4，3： | 9 |
| 14 | CD4^(+)CD25^(+)but not CD4^(+)Foxp3^(+) T cells as a regulatory subset in primary biliary cirrhosis显示文摘Increasing evidence indicates a role for regulatory T cells(Tregs)in the immune response and in autoimmune diseases,but the role of Tregs and cytokines in autoimmune hepatic diseases remains largely unclear and controversial,especially in patients with primary biliary cirrhosis(PBC).This study was undertaken to investigate Tregs and different cytokines in the liver and peripheral blood of PBC patients.We found that these patients demonstrated a reduction of CD4^(+)CD25^(+) T cells but elevated CD4^(+)Foxp3^(+) T cells in peripheral blood mononuclear cells(PBMCs)and CD41 T cells.The percentage of CD4^(+)CD25^(+) T cells in PBMCs was negatively correlated with elevated plasma interferon(IFN)-c levels.A liver-specific analysis showed that the frequency of Foxp31 Tregs,transforming growth factor(TGF)-b1 and IFN-c were increased in PBC patients.Our findings suggest that an imbalance between CD4^(+)CD25^(+) Tregs and cytotoxic cytokines plays a crucial role in the pathogenesis of PBC while the role of Foxp3 needs further investigation. | Dandan Wang Huayong Zhang Jun Liang Zhifeng Gu Qiang Zhou Xiangshan Fan Yayi Hou Lingyun Sun | 2010 | Cellular & Molecular Immunology2010，7，6： | 9 |
| 15 | An elastic-plastic contact model for line contact structures显示文摘Although numerical simulation tools are now very powerful,the development of analytical models is very important for the prediction of the mechanical behaviour of line contact structures for deeply understanding contact problems and engineering applications.For the line contact structures widely used in the engineering field,few analytical models are available for predicting the mechanical behaviour when the structures deform plastically,as the classic Hertz’s theory would be invalid.Thus,the present study proposed an elastic-plastic model for line contact structures based on the understanding of the yield mechanism.A mathematical expression describing the global relationship between load history and contact width evolution of line contact structures was obtained.The proposed model was verified through an actual line contact test and a corresponding numerical simulation.The results confirmed that this model can be used to accurately predict the elastic-plastic mechanical behaviour of a line contact structure. | Haibin Zhu Yingtao Zhao Zhifeng He Ruinan Zhang Shaopeng Ma | 2018 | Science China(Physics,Mechanics & Astronomy)2018，61，5： | 8 |
| 16 | Responses of activities, abundances and community structures of soil denitrifiers to short-term mercury stress显示文摘The responses of activities, abundances and community structures of soil denitrifiers to mercury (Hg) stress were investigated through a short-term incubation experiment. Four soil treatments with different concentrations of Hg (CK, Hg25, Hg50, and Hg100, denoted as 0, 25, 50, and 100 mg Hg/kg dry soil, respectively) were incubated for 28 days. Soil denitrification enzyme activity (DEA) was measured at day 3, 7 and 28. The abundances and community structures of two denitrification concerning genes, nirS (cd1-nitrite reductase gene) and nosZ (nitrous oxide reductase gene), were analyzed using real-time PCR and denaturing gradient gel electrophoresis (DGGE). Results showed that soil DEA was significantly stimulated in the treatments of Hg25 and Hg50 compared with others at day 7. Meanwhile, no difference in the abundances of soil nirS and nosZ was found between Hg spiked treatments and CK, except the lower abundance of nirS (P < 0.05) in the Hg added treatments compared with that in the CK at day 28. The community structures of denitrifiers based on nirS gene presented obvious change at day 7 along with the Hg additions, however, no variation was found in all treatments based on the nosZ gene. The results indicated that Hg (Hg25 and Hg50) had a strongly short-term stimulation on soil DEA, and nirS gene is more sensitive than nosZ gene to Hg stress. | Zhifeng Zhou Yuanming Zheng Jupei Shen Limei Zhang Yurong Liu Jizheng He | 2012 | Journal of Environmental Sciences2012，24，3： | 8 |
| 17 | JAK-STAT signaling mediates the senescence of bone marrow-mesenchymal stem cells from systemic lupus erythematosus patients显示文摘以前的研究揭示了那根骨头从病人们早展出了的全身的豺狼座 erythematosus (SLE ) 的髓间充质的干细胞(BM-MSCs ) 老朽签名,它可以参予 SLE 的发展。然而,关于这现象的分子的机制充分没被阐明。在当前的学习,我们试图调查表明发信号的抄写(STAT ) 的变换器和使活跃之物的 Janus kinase (JAK ) 是否调停了从 SLE 病人的 BM-MSCs 的老朽。12 个女 SLE 病人和健康题目在学习被注册。所有 BM-MSCs 被密度坡度 centrifugation 孤立。西方的污点分析被用来测试表明分子的 JAK-STAT 的表示。我们由流动 cytometry 观察了房间,由染色的 F 肌动朊的细胞骨架结构的变化,和房间周期的分发的女郎的活动。从 SLE 病人的 BM-MSCs 显示出表明 transduction, phosphorylated JAK2 的高水平,和 STAT3 的 JAK-STAT 的老朽,和反常激活的突出的特征。在在正常 MSC 的 IFN- 的刺激以后,发信号的 JAK-STAT 被激活。房间体积和联系老朽的牛乳糖的数字(SA -- 女郎) 在 SLE BM-MSCs 积极被增加。细胞骨架的组织是将近混乱的。房间增长的率被减少。AG490, JAK2 的禁止者,并且在 BM-MSCs 的 STAT3 击倒,能显著地颠倒老朽。在摘要,我们的学习显示 JAK-STAT 发信号小径可以在 SLE BM-MSCs 的老朽起一个关键作用。 | Juan Ji Yeqing Wu Yan Meng Lijuan Zhang Guijuan Feng Yunfei Xia Wenrong Xue Shuyang Zhao Zhifeng Gu Xiaoyi Shao | 2017 | Acta Biochimica et Biophysica Sinica2017，49，3： | 8 |
| 18 | Turnover and loss of nitrogenous compounds during composting of food wastes显示文摘Few people have so far explored into the research of the dynamics of various nitrogenous compounds(including water-soluble nitrogen)in composting of food wastes.This study aimed to investigate the solid-phase nitrogen,water-soluble nitrogen,nitrogen loss together with ammonia volatilization in the process of food wastes composting.A laboratory scale static aerobic reactor in the experiment was employed in the composting process of a synthetic food waste,in which sawdust was used as the litter amendment.In the experiment,oxygen was supplied by continuous forced ventilation for 15 days.The results have shown that the concentrations of total nitrogen and organic nitrogen decrease significantly in the composting process,whereasNH_(4)^(+)-N concentration increases together with little fluctuation in NO_(3)^(-)-N.After composting,the total content of the water-soluble nitrogen compounds in the compost greatly increased,the total nitrogen loss amounted to 50% of the initial nitrogen,mainly attributed to ammonia volatilization.56.7% of the total ammonia volatilization occurred in the middle and late composting of the thermophilic stage.This suggested that the control at the middle and late composting of thermophilic stage is the key to nitrogen loss in the food waste compost. | Yanmei YANG Xiangfeng ZHANG Zhifeng YANG Beidou XI Hongliang LIU | 2008 | Frontiers of Environmental Science & Engineering2008，2，2： | 8 |
| 19 | Assessing genetic diversity of wild populations of Japanese flounderusing AFLP markers显示文摘Amplified fragment length polymorphism (AFLP) analysis was used to evaluate the genetic diversity of four wild geographical populations of Japanese flounder (Paralichthys olivaceus). A total of 775 loci (58.32% of which was polymorphic) in the range between 100 and 1 300 base pairs were detected from 110 individuals using seven primer combinations. The percentage of polymorphic loci detected by single primer combination for each population was calculated, ranging from 19.59% to 53.33%. Genetic similarities within and among the populations were calculated from the binary matrices of presence-absence. Phylogenetic tree of four populations was constructed by using the UPGMA method using PHYLIP Version 3.5. According to intrapopulation genetic similarities, CW population displayed the highest genetic diversity value and KY population had the lowest genetic diversity value. The distance between CW and CF populations was the farthest, which was possibly resulted from the farthest distance of Weihai of Shandong and Fujian of China compared with the geographical distance between other locations of populations. The subpopulation differentiation value (G_ st ) is 0.356 5, showing a certain extent of differentiation among the four geographical populations. AFLP technology was confirmed to be an effective tool to assess within-and among-population genetic diversity of Japanese flounder. The present survey provided significant insights for research in the Japanese flounder breeding program. | XU Xiaofei ZHANG Quanqi WANG Zhigang QI Jie ZHANG Zhifeng BAO Zhenmin Heisuke Nakagawa | 2006 | Acta Oceanologica Sinica2006，25，3： | 7 |
| 20 | Effects of copper and zirconium contents on microstructure and magnetic properties of Sm(Co,Fe,Cu,Zr)_(z)magnets with high iron content显示文摘The evolution of the microstructure,microchemistry and magnetic properties of the Sm(CobalFe_(0.28)-CuyZrx)_(7.6)magnets with different Zr and Cu contents was investigated.It is found that the coercivity of the Sm(Co,Fe,Cu,Zr)_(z)magnets is sensitive to Zr content.The deficiency of Zr content causes heterogeneity of Cu and Fe distributions,while an excessive Zr content leads to the formation of a SmCoZr impurity phase.The cellular structure and distribution of Cu concentration.gradient between the cell boundary phase and cell pha se are destroyed by inappropriate Zr content,which results in a reduction of coercivity.The Cu concentration difference between the cell boundary phase and cell phase increases with increasing Cu content.The coercivity of the Sm(CobalFe_(0.28)CuyZ_(r0.02))_(7.6)magnets increases from 10.4 to 25.4 kOe for y=0.05 and y=0.07.However,the excess of Cu element destroys the cell boundary phase and enlarges the cell size,resulting in a significant decrease of squareness and energy density.The optimum performance(remanence of 11.4 kG,coercivity of 25.4 kOe,maximum magnetic ene rgy product of 30.4 MGOe)was obtained for the Sm(Co_(0.63)Fe_(0.28)Cu_(0.07)Zr_(0.02))_(7.6)magnet. | Zhifeng Shang Dongtao Zhang Zhihong Xie Yunqiao Wang Muhammad Haseeb Pengbiao Qiao Weiqiang Liu Ming Yue | 2021 | Journal of Rare Earths2021，39，2： | 7 |
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