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| 1 | High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes显示文摘Screening gene function in vivo is a powerful approach to discover novel drug targets. We present high-throughput screening(HTS) data for 3 762 distinct global gene knockout(KO) mouse lines with viable adult homozygous mice generated using either gene-trap or homologous recombination technologies. Bone mass was determined from DEXA scans of male and female mice at 14 weeks of age and by microCT analyses of bones from male mice at 16 weeks of age. Wild-type(WT) cagemates/littermates were examined for each gene KO. Lethality was observed in an additional 850 KO lines. Since primary HTS are susceptible to false positive findings, additional cohorts of mice from KO lines with intriguing HTS bone data were examined. Aging,ovariectomy, histomorphometry and bone strength studies were performed and possible non-skeletal phenotypes were explored. Together, these screens identified multiple genes affecting bone mass: 23 previously reported genes(Calcr, Cebpb, Crtap, Dcstamp, Dkk1, Duoxa2, Enpp1, Fgf23, Kiss1/Kiss1 r, Kl(Klotho),Lrp5, Mstn, Neo1, Npr2, Ostm1, Postn, Sfrp4, Slc30a5, Slc39a13, Sost, Sumf1, Src, Wnt10b), five novel genes extensively characterized(Cldn18, Fam20 c, Lrrk1, Sgpl1, Wnt16), five novel genes with preliminary characterization(Agpat2, Rassf5, Slc10a7, Slc26a7, Slc30a10) and three novel undisclosed genes coding for potential osteoporosis drug targets. | Robert Brommage Jeff Liu Gwenn M Hansen Laura L Kirkpatrick David G Potter Arthur T Ss Brian Zambrowicz David R Powell Peter Vogel | 2014 | Bone Research2014，2，3： | 7 |
| 2 | Identification of osteopontin as the most consistently over-expressed gene in intrahepatic cholangiocarcinoma: Detection by oligonucleotide microarray and real-time PCR analysis显示文摘AIM: To investigate the molecular pathways involved in human cholangiocarcinogenesis by gene expression profiling. METHODS: Oligonucleotide arrays (Affymetrix U133A) were used to establish a specific gene expression profile of intrahepatic CCC in comparison to corresponding non- malignant liver tissue. To validate the expression values of the most overexpressed genes, RT-PCR experiments were performed. RESULTS: Five hundred and fifty-two statistically differentially expressed genes/ESTs (221 probes significantly up-regulated, 331 probes down-regulated; P < 0.05; fold change > 2; ≥ 70%) were identified. Using these data and two-dimensional cluster analysis,a specific gene expression profile was obtained allowing fast and reproducible differentiation of CCC, which was confirmed by supervised neuronal network modelling. The most consistently overexpressed gene (median fold change 33.5, significantly overexpressed in 100%) encoded osteopontin. Furthermore, an association of various genes with the histopathological grading could be demonstrated. CONCLUSION: A highly specific gene expression profile for intrahepatic CCC was identified, allowing for its fast and reproducible discrimination against non- malignant liver tissue and other liver masses. The most overexpressed gene in intrahepatic CCC was the gene encoding osteopontin. These data may lead to a better understanding of human cholangiocarcinogenesis. | Holger G Hass Oliver Nehls Juergen Jobst Andrea Frilling Ulrich Vogel Stephan Kaiser | 2008 | World Journal of Gastroenterology2008，14，16： | 4 |
| 3 | Surgery for Ductal Adenocarcinoma of the Pancreatic Head: Staging, Complications, and Survival after Regional versus Extended Lymphadenectomy显示文摘 | Doris Henne-Bruns Ilka Vogel Jutta Lüttges Günter Kl?ppel Bernd Kremer | 2000 | World Journal of Surgery2000，，5： | 2 |
| 4 | Towards a structure-function analysis of bovine lactoferricin and related tryptophan and argini-necontaining peptides 显示文摘 | Vogel H J Schibli D J Jing W G | 2002 | Biochemistry and Cell Biology2002，80，1： | 1 |
| 5 | Implant-retained mandibular overdentures with Branemark System MKII implants:A prospective comparative study between delayed and immediate loading显示文摘 | Chiapasco M Abati S Romeo E Vogel G | | 0，，： | 1 |
| 6 | Roles of the CBF2 and ZAT12 transcription factors in configuring the low temperature transcriptome of Arabldopsis 显示文摘 | Vogel J T Zarka D G Van Buskirk H A | 2005 | Plant J2005，41，： | 1 |
| 7 | DNA repair eapacity: inennsisteney between effect of over-expression of five NER genes and the correlation to mRNA levels in primary lymphoeytes 显示文摘 | VOGEL U DYBDAHL M FRENTZ G | 2000 | Mutat Res2000，461，3： | 1 |
| 8 | Isotopic equilibrium between shells and their environment显示文摘 | Mook W G Vogel J C | 1968 | Science1968，159，： | 1 |
| 9 | Carbon distribution and aboveground net primary production in aspen,jack pine,and black spruce stands in Saskatchewan and Manitoba,Canada显示文摘 | Gower S T Vogel J G Norman J M | 1997 | J Geophys Res1997，102，29： | 1 |
| 10 | Vertical distraction osteogenesis of edentulous ridges for improvement of oral implant positioning : a clinical report of preliminary results 显示文摘 | Chiapasco M Romeo E Vogel G | 2001 | Int J Oral Maxillofac Implants2001，16，1： | 1 |
| 11 | Sequence offers clues to deadly flu 显示文摘 | Vogel G | 1998 | Science1998，279，： | 1 |
| 12 | Roles of the CBF2 and ZAT 12 transcription factors in configuring the 10w temperature transcriptome of Arabidopsis显示文摘 | Vogel J T Zarka D G Van Buskirk H A | 2005 | Plant J2005，41，： | 1 |
| 13 | Gold medal from cellular trash显示文摘 | Vogel G Nobel P | 2004 | Science2004，306，3： | 1 |
| 14 | Harnessing the powe of stem cells显示文摘 | Vogel G | | 0，，： | 1 |
| 15 | Oscillation-free operator splitting method for semilinear diffusion equations显示文摘 | Harwood R C Zhang L K Vogel G M | 2013 | Journal of Ap- plied Mathematics and Computation2013，18，7： | 1 |
| 16 | Quasi-crystalline grain-boundary phase in the magnesium die-cast alloy ZA85 显示文摘 | VOGEL M KRAFT O DEHM G ARZT E | 2001 | Scripta Mater2001，45，5： | 1 |
| 17 | Changes in flow - mediated brachial artery vasoactivity with lowering of desirable cholesterol levels in healthy midder-aged men显示文摘 | Vogel R A Corretti M C Plotnick G D | 1996 | J Am Cardi1996，77，： | 1 |
| 18 | Breakthrough of the Year: Capturing thePromise of Youth显示文摘 | Vogel G | 1999 | Science1999，286，5448： | 1 |
| 19 | Neural activity predicts individual differences in visual working memory capacity显示文摘 | Vogel E K Machizawa M G | 2004 | Nature2004，428，： | 1 |
| 20 | Simulation of Acid Washing of Municipal Solid Waste Incineration Fly Ash in Order to Remove Heavy Metals显示文摘 | Bvander Bruggen G Vogels P Van Herck | 1998 | Hazardous Materials1998，57，： | 1 |
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