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| 1 | Ultrafast photonic PCR显示文摘Nucleic acid amplification and quantification via polymerase chain reaction(PCR)is one of the most sensitive and powerful tools for clinical laboratories,precision medicine,personalized medicine,agricultural science,forensic science and environmental science.Ultrafast multiplex PCR,characterized by low power consumption,compact size and simple operation,is ideal for timely diagnosis at the point-of-care(POC).Although several fast/ultrafast PCR methods have been proposed,the use of a simple and robust PCR thermal cycler remains challenging for POC testing.Here,we present an ultrafast photonic PCR method using plasmonic photothermal light-to-heat conversion via photon–electron–phonon coupling.We demonstrate an efficient photonic heat converter using a thin gold(Au)film due to its plasmon-assisted high optical absorption(approximately 65%at 450 nm,the peak wavelength of heat source light-emitting diodes(LEDs)).The plasmon-excited Au film is capable of rapidly heating the surrounding solution to over 150℃ within 3 min.Using this method,ultrafast thermal cycling(30 cycles;heating and cooling rate of 12.7960.93℃ s^(-1) and 6.660.29℃ s^(-1),respectively)from 55℃(temperature of annealing)to 95℃(temperature of denaturation)is accomplished within 5 min.Using photonic PCR thermal cycles,we demonstrate here successful nucleic acid(λ-DNA)amplification.Our simple,robust and low cost approach to ultrafast PCR using an efficient photonic-based heating procedure could be generally integrated into a variety of devices or procedures,including on-chip thermal lysis and heating for isothermal amplifications. | Jun Ho Son Byungrae Cho SoonGweon Hong Sang Hun Lee Ori Hoxha Amanda J Haack Luke P Lee | 2015 | Light(Science & Applications)2015，4，1： | 2 |
| 2 | Sedimentation on the Lonian active margin(Hellenicare)-provenanee of sediments and mechanisms of deposition 显示文摘 | Ingersoll H Monaco A Vitt ori J | 1987 | Sedimentary Geology1987，28，4： | 1 |
| 3 | A Normative Theory of the Clean Hands De- fence 显示文摘 | Ori J Herstein | 2011 | Legal Theory2011，9，17： | 1 |
| 4 | Survival of copper-expose juvenile fathead minnows (Pimephales promelas)differs among allozyme genotypes显示文摘 | Schlueter M A Guttman S I Oris J T | 1995 | Enviro Toxicol Chem1995，14，10： | 1 |
| 5 | Differential survivorship among allozyme genotypes of Hyalella azteca exposed to cadmium, zinc or low pH 显示文摘 | Duan Y Guttman S I Oris J T | 2001 | Aquatic Toxicology2001，54，12： | 1 |
| 6 | Comparison of P-4501A1 Monooxygenase induction in gizzard shad (Dorosoma cepedianum) following intraperitoneal injection or continuous waterborne-exposure with benzopyrene:Temporal and dose-dependent studies显示文摘 | LEVINE S L ORIS J T WISSING T E | 1994 | Aquatic Toxicol1994，，30： | 1 |
| 7 | Modelling the influence of trapping on hydrogen permeation in metals显示文摘 | Fischer F D M ori G Svoboda J | 2013 | Corrosion Science2013，76，： | 1 |
| 8 | What do you think would make you happier? what do you think you choose? 显示文摘 | DANIEL J B ORI H MILES S K ALEX R J | 2012 | American Economic Review2012，102，5： | 1 |
| 9 | Anthracene reduces reproductive potential andis maternally transferred during longterm exposure in fathead minnows显示文摘 | Tilghman H A Oris J T | 1991 | Aquat Toxicol1991，19，： | 1 |
| 10 | Hemodynamic changes during laparoscopic cholecystectomy显示文摘 | ORIS J L NOIROT D P LEGRAND M J | 1993 | Anesth Analg1993，76，： | 1 |
| 11 | Laboratory and field validation of multiple molecular biomarkers of contaminant ex- posure in rainbow trout( Oncorhynchus mykiss) 显示文摘 | MCCLAIN J S ORIS J T BURTON G A | 2003 | Environ Toxi- col Chem2003，22，2： | 1 |
| 12 | Mitochondrial protectant pramipexole prevents sex-specific long-term cognitive impairment from early anaesthesia exposure in rats显示文摘 | Boscolo A Ori C Bennett J | | 0，，1： | 1 |
| 13 | The role of pH dynamics and the Na^+/ H^+ antiporter in the etiopathogenesis and treatment of cancer: Two faces of the same coin-one single nature显示文摘 | HARGUIDEY S ORI V PEDRAZ J L | 2005 | Biochem et Bioph2005，1756，1： | 1 |
| 14 | Solid- state wetting and spreading of tin (Sn) on aluminium interfaces显示文摘 | Eisenmenger- Sittner C Bangert H Bergauer A Brenner J St ori H Barna P B | 2003 | Vacuum2003，71，： | 1 |
| 15 | Differential survivorship among allozyme genotypes of Hyalella azteca exposed to cadmium, zinc or low pH显示文摘 | Duan Y H Guttman S I Oris J T | 2001 | A quatic Toxicology2001，54，： | 1 |
| 16 | Credit scoring for mi- crofinance:is it worth it? 显示文摘 | J ORIS V G WOUTER V PIET S etal | 2012 | International Journal of Finance Economics2012，17，2： | 1 |
| 17 | Survival of copper-expose juvenile fathead minnows(Pimephales promelas )differs among allozyme genotypes显示文摘 | Schlueter M A Guttman S I Oris J T | 1995 | Enviro Toxicol Chem1995，14，10： | 1 |
| 18 | Adjacent vertex distinguishing edgecolorings显示文摘 | Balister P N Gy ori E Lehel J et ol | 2007 | SIAM J Discrete Math2007，21，： | 1 |
| 19 | An ultrastructural examination of the mode of UV_induced toxic action of fluoranthene in the fathead minnow,Pimphales promelas显示文摘 | Weinstein J E Oris J T Taylor D H | 1997 | Aquat Toxicol1997，39，： | 1 |
| 20 | Anticomplement activity of triterpenoids from the spores of Ganoderma lucidum显示文摘 | MIN B S GAO J J HATI'ORI M | 2001 | Planta Med2001，67，： | 1 |
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