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| 1 | Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy显示文摘Conventional immunohistochemistry(IHC)is a widely used diagnostic technique in tissue pathology.However,this technique is associated with a number of limitations,including high inter-observer variability and the capacity to label only one marker per tissue section.This review details various highly multiplexed techniques that have emerged to circumvent these constraints,allowing simultaneous detection of multiple markers on a single tissue section and the comprehensive study of cell composition,cellular functional and cell-cell interactions.Among these techniques,multiplex Immunohistochemistry/Immunofluorescence(mIHC/IF)has emerged to be particularly promising.mIHC/IF provides high-throughput multiplex staining and standardized quantitative analysis for highly reproducible,efficient and cost-effective tissue studies.This technique has immediate potential for translational research and clinical practice,particularly in the era of cancer immunotherapy. | Wei Chang Colin Tan Sanjna Nilesh Nerurkar Hai Yun Cai Harry Ho Man Ng Duoduo Wu Yu Ting Felicia Wee Jeffrey Chun Tatt Lim Joe Yeong Tony Kiat Hon Lim | 2020 | Cancer Communications2020，40，4： | 11 |
| 2 | 肿瘤免疫治疗时代的多重免疫组织化学/免疫荧光技术显示文摘常规免疫组织化学(immunohistochemistry,IHC)是组织病理学中广泛应用的诊断技术。但该技术存在诸多局限性,包括观察者间的变异性高,每张组织切片仅能标记一个标志物。本文详细描述了各种多重检测技术,这些技术克服了上述局限性,可在单张切片上同时检测多个标志物,可对细胞组成、细胞功能和细胞间相互作用进行整合研究。在这些技术中,多重免疫组织化学/免疫荧光(multiplex immunohistochemistry/immunofluorescence,mIHC/IF)已成为最具应用前景的技术。mIHC/IF为开展高重复性、高效、高性价比的组织研究提供了高通量多重染色技术和标准化的定量分析。该技术在转化研究和临床实践中,尤其是肿瘤免疫治疗中极具应用价值。 | Wei Chang Colin Tan Sanjna Nilesh Nerurkar Hai Yun Cai Harry Ho Man Ng Duoduo Wu Yu Ting Felicia Wee Jeffrey Chun Tatt Lim Joe Yeong Tony Kiat Hon Lim | 2021 | 癌症2021，40，9： | 2 |
| 3 | In vivo activation of peroxisome proliferator-activated receptor-delta protects the heart from isch- emia/reperfusion injury in Zucker fatty rats 显示文摘 | Yue TL Nerurkar SS Bao W | 2008 | J Pharmacol Exp- Ther2008，325，2： | 1 |
| 4 | Bitter melon: antagonist to cancer 显示文摘 | Nerurkar P Ray RB | 2010 | Pharm Res2010，27，6： | 1 |
| 5 | Methoxyresorufin and benzyloxyresorufin:substrates preferentially metabolized by cytochromes P450 1A2 and 2B,respectively,in the rat and mouse显示文摘 | NERURKAR PV PARK SS THOMAS PE | 1993 | Biochem Pharmacol1993，46，5： | 1 |
| 6 | In vivo activation of peroxisome proliferator-activated receptor-delta protects the heart from isehemia/ reperfusion injury in Zueker fatty rats 显示文摘 | Yue TL Nerurkar SS Bao W | 2008 | J Pharmaeol Exp Ther2008，325，： | 1 |
| 7 | Mechanics of oriented electrospun nanofibrous scaffolds for annulus fibrosus tissue engi- neering显示文摘 | Nerurkar NL Elliott DM Mauck RL | 2007 | Orthop Res2007，25，8： | 1 |
| 8 | Bitter melon: antagonist to cancer显示文摘 | Nerurkar P Ray RB | 2010 | Pharm Res2010，27，6： | 1 |
| 9 | Homologous structure-function relationships between native fibrocartilage and tissue engineered from MSC-seeded nanofibrous scaffolds显示文摘 | Nerurkar NL Han W Mauck RL | 2011 | Biomaterials2011，32，2： | 1 |
| 10 | Ex-pression of survivin and p53 proteins and their correlationwith hormone receptor status in Indian breast cancer pa-tients显示文摘 | Ranade K J Nerurkar A V Phulpagar M D | 2009 | Indian J Med Sci2009，63，11： | 1 |
| 11 | al namic culture enhances stem cell infiltration and modulates extracellular matrix production on aligned electrospun nanofibrous scaffolds显示文摘 | Nerurkar NL Sen S Baker BM | 2011 | Acts Biomater2011，7，2： | 1 |
| 12 | In vivo activation of peroxisome proliferator-activated receptor-delta protects the heart from ischemia/reperfusion injury in Zucker fatty rats显示文摘 | Yue T L Nerurkar S S Bao W | 2008 | J Pharmacol Exp Ther2008，325，2： | 1 |
| 13 | In vivo activation of peroxisome proliferator-activated receptor-delta protects the heart from ischemia/reperfusion injury in Zucker fatty rats显示文摘 | Yue TL Nerurkar SS Bao W | 2008 | J Pharmacol Exp Ther2008，325，2： | 1 |
| 14 | Web user interface design forgotten lessons显示文摘 | NERURKAR U | 2001 | IEEE Software2001，18，6： | 1 |
| 15 | ISSLS prize winner: integrating theoretical and experimental methods for functional tis- sue engineering of the annulus fibrosus 显示文摘 | Nerurkar NL Manck RL Elliott DM | 2008 | Spine ( Phila Pa 1976)2008，33，25： | 1 |
| 16 | CYP1A1,GSTMI,and GSTP1 genetic polymorphisms and urinary 1-hydroxypyrene excretion in non-occupationally exposed individuals显示文摘 | Nerurkar PV Okinaka L Aoki C | 2000 | Cancer Epidem Biomar2000，9，： | 1 |
| 17 | ISSLS prize winner:integrating theoretical and experimental methods for functional tissue engineering of the annulus fibrosus显示文摘 | Nerurkar NL Mauck RL Elliott DM | 2008 | Spine2008，33，25： | 1 |
| 18 | Biosystem develop- ment for microbial enhanced oil recovery (MEOR) 显示文摘 | Nerurkar A S Suthar H G Desai A J | 2012 | Microorganisms in Sustainable Agriculture and Biotech- nology2012，2，： | 1 |
| 19 | Multi-scale structural and tensile mechanical response of annulus fibrosus to osmotic loading显示文摘 | Han WM Nerurkar NL Smith I J | 2012 | Ann Biomed Eng2012，40，7： | 1 |
| 20 | Nanofbrus biologic laminates replicate the form and function of the annulus fibrosus显示文摘 | Nerurkar NL Baker BM Sen S | 2009 | Nat Mater2009，8，12： | 1 |
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