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| 1 | Effects of glutamine supplementation on gut barrier,glutathione content and acute phase response in malnourished rats during inflammatory shock显示文摘AIM: To evaluate the effect of glutamine on intestinal mucosa integrity,glutathione stores and acute phase response in protein-depleted rats during an inflammatory shock. METHODS: Plasma acute phase proteins (APP),jejunal APP mRNA levels,liver and jejunal glutathione concentrations were measured before and one,three and seven days after turpentine injection in 4 groups of control,protein-restricted,protein-restricted rats supplemented with glutamine or protein powder. Bacterial translocation in mesenteric lymph nodes and intestinal morphology were also assessed. RESULTS: Protein deprivation and turpentine injection significantly reduced jejunal villus height,and crypt depths. Mucosal glutathione concentration significantly decreased in protein-restricted rats. Before turpentine oil,glutamine supplementation restored villus heights and glutathione concentration (3.24 ± 1.05 vs 1.72 ± 0.46 μmol/g tissue,P < 0.05) in the jejunum,whereas in the liver glutathione remained low. Glutamine markedly increased jejunal α1-acid glycoprotein mRNA level after turpentine oil but did not affect its plasma concentration. Bacterial translocation in protein-restricted rats was not prevented by glutamine or protein powder supplementation. CONCLUSION: Glutamine restored gut glutathione stores and villus heights in malnourished rats but had no preventive effect on bacterial translocation in our model. | Liliana Belmonte Mose Co■ffier Florence Le Pessot Olga Miralles-Barrachina Martine Hiron Antony Leplingard Jean-Franois Lemeland Bernadette Hecketsweiler Maryvonne Daveau Philippe Ducrotté Pierre Déchelotte | 2007 | World Journal of Gastroenterology2007，13，20： | 6 |
| 2 | 2,4,6-trinitrobenzene sulfonic acid-induced chronic colitis with fibrosis and modulation of TGF-β1 signaling显示文摘AIM:To investigate whether targeting proteasome might reverse intestinal fibrosis in rats.METHODS:Chronic colitis was induced in rats by repeated administration of increasing dose of2,4,6-trinitrobenzene sulfonic acid(TNBS,15,30,45,60,60,60 mg)by rectal injection for 6 wk(from day0 to day 35),while control rats received the vehicle.TNBS+bortezomib(BTZ)rats received intraperitoneal injections of BTZ twice weekly(from day 37 to day44)at a dose of 25 mg/kg,whereas the control and TNBS groups received the same amount of the vehicle.Histologic scoring of inflammation and fibrosis was performed.Colonic production of transforming growth factor(TGF)-βwas measured by ELISA.Colon fibrosisrelated proteins such as phospho-p38,phosphoSMAD2/3,Akt and peroxisome proliferator activated receptorγ(PPARγ)were studied by western blot.Expression of the tight junction proteins,occludin and claudin-1,were assessed by Western blot.Colon proteasome activities(chymotrypsin-like and trypsinlike activities)were assessed.RESULTS:TNBS-treated rats had a higher colon weight/length ratio compared to control rats(P<0.01).Furthermore,fibrosis and inflammation scores were higher in TNBS-treated rats compared to control rats(P<0.01 for both).Colonic production of TGF-βproduction tended to be higher in TNBS-treated rats(P<0.06).Fibrosis-related proteins such as phospho-p38,phospho-SMAD2/3,and PPARγwere significantly higher in TNBS-treated rats compared to control rats(all P<0.05).TNBS rats had a higher expression of Akt compared to control rats(P<0.01).Tight junction proteins were modified by repeated TNBS challenge:colon occludin expression rose significantly(P<0.01),whereas claudin-1 expression fell(P<0.01).Bortezomib inhibition significantly decreased chymotrypsin-like activity(P<0.05),but had no significant effect on trypsin-like activity(P>0.05).In contrast,bortezomib had no effect on other studied parameters such as fibrosis score,TGF-βsignaling,or tight junction expression(P>0.05 for all).CONCLUSION:Rats with TNBS-induced chronic colitis exhibited colon fibrosis associated with higher TGF-βsignaling.Proteasome inhibition by bortezomib had no effect on fibrosis in our experimental conditions. | Emilien Loeuillard Julien Bertrand Anni Herranen Chloé Melchior Charlène Guérin Mo?se Co?ffier Moutaz Aziz Pierre Déchelotte Guillaume Savoye Rachel Marion-Letellier | 2014 | World Journal of Gastroenterology2014，20，48： | 4 |
| 3 | Juvenile ferric iron prevents microbiota dysbiosis and colitis in adult rodents显示文摘AIM: To assess whether juvenile chronic ferric iron ingestion limit colitis and dysbiosis at adulthood in rats and mice. METHODS: Two sets of experiments were designed. In the first set, recently weaned mice were either orally administered ferrous (Fe2+) iron salt or ferric (Fe3+) microencapsulated iron for 6 wk. The last week of experiments trinitrobenzene sulfonic acid (TNBS) colitis was induced. In the second set, juvenile rats received the microencapsulated ferric iron for 6 wk and were also submitted to TNBS colitis during the last week of experiments. In both sets of experiments, animals were sacrificed 7 d after TNBS instillation. Severity of the inflammation was assessed by scoring macroscopic lesions and quantifying colonic myeloperoxidase (MPO) activity. Alteration of the microflora profile was estimated usingquantitative polymerase chain reaction (qPCR) by measuring the evolution of total caecal microflora, Bacteroidetes, Firmicutes and enterobacteria. RESULTS: Neither ferrous nor ferric iron daily exposures at the juvenile period result in any effect in control animals at adulthood although ferrous iron repeated administration in infancy limited weight gain. Ferrous iron was unable to limit the experimental colitis (1.71 ± 0.27 MPO U/mg proteinvs 2.47 ± 0.22 MPO U/mg protein in colitic mice). In contrast, ferric iron significantly prevented the increase of MPO activity (1.64 ± 0.14 MPO U/mg protein) in TNBS-induced colitis. Moreover, this positive effect was observed at both the doses of ferric iron used (75 and 150 mg/kg per day po - 6 wk). In the study we also compared, in both rats and mice, the consequences of chronic repeated low level exposure to ferric iron (75 mg/kg per day po - 6 wk) on TNBS-induced colitis and its related dysbiosis. We confirmed that ferric iron limited the TNBS-induced increase of MPO activity in both the rodent species. Furthermore, we assessed the ferric iron incidence on TNBS-induced intestinal microbiota dysbiosis. At first, we needed to optimize the isolation and quantify DNA copy numbers using standard curves to perform by qPCR this interspecies comparison. Using this approach, we determined that total microflora was similar in control rats and mice and was mainly composed of Firmicutes and Bacteroidetes at a ratio of 10/1. Ferric juvenile administration did not modify the microflora profile in control animals. Total microflora numbers remained unchanged whichever experimental conditions studied. Following TNBS-induced colitis, the Firmicutes/Bacteroidetes ratio was altered resulting in a decrease of the Firmicutes numbers and an increase of the Bacteroidetes numbers typical of a gut inflammatory reaction. In parallel, the subdominant population, the enterobacteria was also increased. However, ferric iron supplementation for the juvenile period prevented the increase of Bacteroidetes and of enterobacteria numbers consecutive to the colitis in both the studied species at adulthood.CONCLUSION: Rats and mice juvenile chronic ferric iron ingestion prevents colitis and dysbiosis at adulthood as assessed by the first interspecies comparison. | Chourouk Ettreiki Pascale Gadonna-Widehem Irène Mangin Mose Coёffier Carine Delayre-Orthez Pauline M Anton | 2012 | World Journal of Gastroenterology2012，18，21： | 3 |
| 4 | Short-term overlap lamivudine treatment with adefovir dipivoxil in patients with lamivudine-resistant chronic hepatitis B显示文摘AIM:To evaluate the efficacy of short-term overlap lamivudine therapy with adefovir in patients with lamivudine-resistant and nave chronic hepatitis B,we compared patients receiving overlap therapy with those receiving adefovir alone. METHODS:Eighty patients who had received lamivudine treatment for various periods and had a lamivudine- resistant liver function abnormality were enrolled.Forty of these patients received adefovir treatment combined with lamivudine treatment for≥2 mo,while the other 40 received adefovir alone.We assessed the levels of hepatitis B virus(HBV)DNA at 0,12 and 48 wk and serum alanine aminotransferase(ALT)levels after 0,12, 24 and 48 wk of adefovir treatment in each group. RESULTS:We found serum ALT became normalized in 72(87.5%)of the 80 patients,and HBV DNA decreased by≥2 log10 copies/mL in 60(75%)of the 80 patients at the end of a 48-wk treatment.HBV DNA levels were not significantly different between the groups.The improvements in serum ALT were also not significantly different between the two groups. CONCLUSION:These findings suggest short-term overlap lamivudine treatment results in no better virological and biological outcomes than non-overlap adefovir monotherapy. | Soon Woo Nam Si Hyun Bae Seung Woo Lee Yeon Soo Kim Sang Bum Kang Jong Young Choi Se Hyun Cho Seung Kew Yoon Joon-Yeol Han Jin Mo Yang Young Suk Lee | 2008 | World Journal of Gastroenterology2008，14，11： | 3 |
| 5 | Oxidative stress-elevated high gamma glutamyl transferase levels, and aging, intake of tropical food plants, migration and visual disability in Central Africans显示文摘·AIM:To investigate the independent pathogenic role of high serum gamma-glutamyl transferase (GGT) levels, sociodemographic data, dietary and environmental risk factors for visual disability (VD). ·METHODS:This was a case-control study, run in 200 black Congolese patients managed in Saint Joseph Hospital Ophthalmology Division from Kinshasa town. Logistic regression model was used to identify determinants of VD (n = 58) among sex, age, cigarette smoking, alcohol abuse, rural-urban migration, education levels, aging ≥60 years, intake of red Beans, Safou fruit and Taro leaves, lipid profile, residence, socioeconomic status, and GGT. ·RESULTS:After adjusting for confounding factors, we identified migration (OR=3.7 95% CI:1.2-11.3; P =0.023), low education level (OR=3.1 95% CI 1.1-8.5; P =0.026), no intake of Safou fruit (OR=34.2 95% CI 11.5-102; P < 0.0001), age ≥60 years (OR=2.5 95% CI 1.01-6.5; P = 0.049), and serum GGT ≥10U/L (OR=3.6 95% CI 1.3-9.6; P = 0.012) as the significant and independent determinants of VD. ·CONCLUSION:VD appears as a major public health problem in Central Africa to be prevented or delayed by control of migration, lifestyle changes, antioxidant supplements, appropriate diet, nutrition education, and blocking of oxidative stress. | Benjamin Longo-Mbenza Mose Mvitu Muaka Etienne Mokondjimobe Dalida Kibokela Ndembe Doris Tulomba Mona Baudouin Buassabu-bu-Tsumbu | 2012 | International Journal of Ophthalmology(English edition)2012，5，4： | 2 |
| 6 | Computed tomographic assessment of fractures of the posterior wall of the acetabulum after operative treatment显示文摘 | Moed BR Carr SE Gruson KI | 2003 | J Bone Joint Surg(Am)2003，85，3： | 1 |
| 7 | Open reduction and internal fixation of posterior wall fractures of the acetabulum显示文摘 | Moed BR Carr SE Watson JT | 2000 | Clin Orthop Relat Res2000，，377： | 1 |
| 8 | Diagnostic use of serum ferritin lev- els to differentiate infectious and noninfectious diseases in patients with Diagnostic use of serum ferritin levels 显示文摘 | Kim SE Kim UJ Jang MO | 2013 | Disease Markers2013，34，3： | 1 |
| 9 | Open reduction and internal fixation of poslerior wall fractures of file acetabulum显示文摘 | Moed BR Carr SE Watson JT | 2000 | Clin Orthop Relat Res2000，，377： | 1 |
| 10 | Results of operative treatment of fractures of the posterior wall of the acetabulum 显示文摘 | Moed BR WillsonCarr SE Watson JT | 2002 | J Bone Joint Surg (Am)2002，84，5： | 1 |
| 11 | Deep fuels desulfurization and denitrogenation using 1-butyl-3-methylimidazolium trifluoromethanesulfonate显示文摘 | Kedra-Krolik K Mutelet F Moǐse J-C | | 0，，04： | 1 |
| 12 | Low monocyte HLA-DR expression helpful to predict outcome in severe sepsis显示文摘 | Perry SE Mo stafa SM Wenstone R | 2003 | Intensive Care Med2003，29，： | 1 |
| 13 | Results of operative treatment of fractures of the pasterior wall of the acetabulum显示文摘 | Moed BR Willsoncarr SE Watson JT | 2002 | Bone Joint Sury (Am)2002，，： | 1 |
| 14 | Results of operative treatment of fractures of the posterior wall of the acetabu- lum显示文摘 | Moed BR Willson Carr SE Watson JT | 2002 | J Bone Joint Surg(Am)2002，84，5： | 1 |
| 15 | Computed tomographic assessment of fractures of posterior wall of the acetabulum atter operative treatment显示文摘 | Moed BR Willson Cart SE Gruson KI | 2003 | Bone Joint Surg (Am)2003，85，： | 1 |
| 16 | Antiviral gene expression in rheumatoid arthritis:role of IKK-and interferon regulatory factor 3显示文摘 | Sweeney SE Mo L Firestein GS | | 0，，3： | 1 |
| 17 | Enteral glutamine infusion modulates ubiquitination of heat shock proteins, Grp-75 and Apg-2, in the human duodenal mucosa显示文摘 | Julien Bertrand Alexis Goichon Philippe Chan Saida Azhar Stéphane Lecleire Nathalie Donnadieu David Vaudry Anne-Fran?oise Cailleux Pierre Déchelotte Mo?se Co?ffier | 2014 | Amino Acids2014，，4： | 1 |
| 18 | Open reduction and internal fixation of posterior wall fractures of the acetabulum显示文摘 | Moed BR Willson Carr SE Tracy Watson J | 2000 | Clin Orthop2000，377，： | 1 |
| 19 | Computed tomographic assessment of fractures of the posterior wall of the acetabulum after operative treatment显示文摘 | Moed BR Carr SE Gruson KI | 2003 | J Bone Joint Surg Am2003，85，3： | 1 |
| 20 | Results of operative treatment of fractures of the posterior wall of the acetabulum显示文摘 | Moed BR WillsonCarr SE Watson JT | 2002 | J Bone Joint Surg (Am)2002，84，： | 1 |
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