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| 1 | HIF-1α-induced expression of m6A reader YTHDF1 drives hypoxia-induced autophagy and malignancy of hepatocellular carcinoma by promoting ATG2A and ATG14 translation显示文摘N6-methyladenosine(m6A),and its reader protein YTHDF1,play a pivotal role in human tumorigenesis by affecting nearly everystage of RNA metabolism.Autophagy activation is one of the ways by which cancer cells survive hypoxia.However,the possibleinvolvement of m6A modification of mRNA in hypoxia-induced autophagy was unexplored in human hepatocellular carcinoma(HCO).In this study,specific variations in YTHDF1 expression were detected in YTHDF1-overexpressing,knockout,and-knockdownHCC cells,HCC organoids,and HCC patient-derived xenograft(PDX)murine models.YTHDF1 expression and hypoxia inducedautophagy were significantly correlated in vitro;signifhcant overexpression of YTHDF1 in HCC tissues was associated with poorprognosis,Multivariate cox regression analysis identihed YTHDF1 expression as an independent prognostic factor in patients withHCC.Multiple HC models conhrmed that YTHDF1 deficiency inhibited HCC autophagy,growth,and metastasis.Luciferase reporterassays and chromatin immunoprecipitation demonstrated that HlIF-1a regulated YTHDF1 transcription by directly binding to itspromoter region under hypoxia.The results of methylated RNA immunoprecipitation sequencing,proteomics,and polysomeprofling indicated that YTHDF1 contibuted to the translation of autophagy-related genes ATG2A and ATG14 by binding to m6A-modifhed ATG2A and ATG14 mRNA,thus facilitating autophagy and autophagy-related malignancy of HCC.Taken together,HlE-1d-induced YTHDF1 expression was associated with hypoxia-induced autophagy and autophagy-related HCC progression via promoting translation of autophagy-related genes ATG2A and ATG14 in a m6A-dependent manner.Our fndings suggest thatYTHDF1 is a potential prognostic biomarker and therapeutic target for patients with HCC. | Qing Li Yong Ni Liren Zhang Runqiu Jiang Jing Xu Hong Yang Yuanchang Hu Jiannan Qiu Liyong Pu Jinhai Tang Xuehao Wang | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 24 |
| 2 | Reactivation of γ-globin expression through Cas9 or base editor to treat β-hemoglobinopathies显示文摘Dear Editor,Mutations in the β-globin gene,the essential component of adult hemoglobin(HbA;a2p2),results in either a production of aberrant sickle hemoglobin(HbS)leading to sickle cell disease(SCD)or an insufficient β-globin synthesis leading to β-thalassemia.These two major forms of β-hemoglobinopathies cause impaired erythropoiesis and life-threatening anemia.Clinical evidence has suggested that reaaivation of fetal γ-globin(HBG)gene expression which is normally silenced after birth by certain genetic mutations can ameliorate the clinical course of β-hemoglobinopathies. | Liren Wang Linxi Li Yanlin Ma Handan Hu Qi Li Yang Yang Wenbang Liu Shuming Yin Wei Li Bin Fu Ryo Kurita Yukio Nakamura Mingyao Liu Yongrong Lai Dali Li | 2020 | Cell Research2020，30，3： | 14 |
| 3 | Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants显示文摘 | Lei Yang Xiaohui Zhang Liren Wang Shuming Yin Biyun Zhu Ling Xie Qiuhui Duan Huiqiong Hu Rui Zheng Yu Wei Liangyue Peng Honghui Han Jiqin Zhang Wenjuan Qiu Hongquan Geng Stefan Siwko Xueli Zhang Mingyao Liu Dali Li | 2018 | Protein & Cell2018，9，9： | 13 |
| 4 | CRISPR/Cas9 system: a powerful technology for in vivo and ex vivo gene therapy显示文摘CRISPR/Cas9 is a versatile genome-editing tool which is widely used for modifying the genome of both prokaryotic and eukaryotic organisms for basic research and applications. An increasing number of reports have demonstrated that CRISPR/Cas9-mediated genome editing is a powerful technology for gene therapy. Here, we review the recent advances in CRISPR/Cas9-mediated gene therapy in animal models via different strategies and discuss the challenges as well as future prospects. | Xiaohui Zhang Liren Wang Mingyao Liu Dali Li | 2017 | Science China(Life Sciences)2017，60，5： | 12 |
| 5 | Gene editing and its applications in biomedicine显示文摘The steady progress in genome editing, especially genome editing based on the use of clustered regularly interspaced short palindromic repeats(CRISPR) and programmable nucleases to make precise modifications to genetic material, has provided enormous opportunities to advance biomedical research and promote human health. The application of these technologies in basic biomedical research has yielded significant advances in identifying and studying key molecular targets relevant to human diseases and their treatment. The clinical translation of genome editing techniques offers unprecedented biomedical engineering capabilities in the diagnosis, prevention, and treatment of disease or disability. Here, we provide a general summary of emerging biomedical applications of genome editing, including open challenges. We also summarize the tools of genome editing and the insights derived from their applications, hoping to accelerate new discoveries and therapies in biomedicine. | Guanglei Li Xiangyang Li Songkuan Zhuang Liren Wang Yifan Zhu Yangcan Chen Wen Sun Zeguang Wu Zhuo Zhou Jia Chen Xingxu Huang Jin Wang Dali Li Wei Li Haoyi Wang Wensheng Wei | 2022 | Science China(Life Sciences)2022，65，4： | 8 |
| 6 | Essential role of the CUL4B ubiquitin ligase in extra-embryonic tissue development during mouse embryogenesis显示文摘CUL4B ubiquitin ligase 基因的变化有原因地被连接到 syndromic X 连接智力迟钝(XLMR ) 。然而,在 neuronal 和发展缺点的 CUL4B 变化的病原的角色没被理解。我们与 Cul4b 的指向的混乱产生了老鼠,并且与显著生长抑制观察了胚胎的致命性并且在胚胎外的纸巾增加了 apoptosis。Cul4b,然而并非它的 paralog Cul4a,在胚胎外的纸巾驿站培植在高水平被表示。在一根胚胎外的房间线的 CUL4B 表示的 Silencing 导致了 CUL4 底层 p21 Cip1/WAF 和 G2/M 房间周期逮捕 Cip1/WAF 。Cul4b 的 Epiblast 特定的删除阻止了胚胎的致命性并且产生了可行 Cul4b 空老鼠。因此,当时在胚胎非必需合适, Cul4b 在胚胎外的纸巾执行一个必要发展角色。我们的学习提供策略产生可行 Cul4b 缺乏的老鼠为潜在的 neuronal 和人的 CUL4B XLMR 病人的行为的缺乏建模。 | Liren Liu Yan Yin Yuewei Li Lisa Prevedel Elizabeth H Lacy Liang Ma Pengbo Zhou | 2012 | Cell Research2012，22，8： | 6 |
| 7 | Total mesorectal excision with or without preoperative chemoradiotherapy for resectable mid/low rectal cancer: a long-term analysis of a prospective, single-center, randomized trial显示文摘Background:The preliminary results of our phase II randomized trial reported comparable functional sphincter pres-ervation rates and short-term survival outcomes between patients undergoing total mesorectal excision(TME)with or without preoperative concurrent chemoradiotherapy(CCRT).We now report the long-term results after a median follow-up of 71 months.Methods:Between March 23,2008 and August 2,2012,192 patients with T3-T4 or node-positive,resectable,mid/low rectal adenocarcinoma were randomly assigned to receive TME with or without preoperative CCRT.The following endpoints were assessed:cumulative rates of local recurrence and distant metastasis,disease-free survival(DFS),and overall survival(OS).Results:The data of 184 eligible patients were analyzed:94 patients in the TME group and 90 patients in the CCRT+TME group.In the whole cohort,the 5-year DFS and OS rates were 84.8%and 85.1%,respectively.The 5-year DFS rates were 85.2%in the CCRT+TME group and 84.3%in the TME group(P=0.969),and the 5-year OS rates were 83.5%in the CCRT+TME group and 86.5%in the TME group(P=0.719).The 5-year cumulative rates of local recur-rence were 6.3%and 5.0%(P=0.681),and the 5-year cumulative rates of distant metastasis were 15.0%and 15.7%(P=0.881)in the CCRT+TME and TME groups,respectively.No significant improvements in 5-year DFS and OS were observed with CCRT by subgroup analyses.Conclusions:Both treatment strategies yielded similar long-term outcomes.A selective policy towards preoperative CCRT is thus recommended for rectal cancer patients if high-quality TME surgery and enhanced chemotherapy can be performed. | Fulong Wang Wenhua Fan Jianhong Peng Zhenhai Lu Zhizhong Pan Liren Li Yuanhong Gao Hui Li Gong Chen Xiaojun Wu Peirong Ding Zhifan Zeng Desen Wan | 2018 | Cancer Communications2018，38，1： | 6 |
| 8 | Bleaching effect and nonvolatile holographic storage in doubly doped LiNbO_3:Fe:Cu crystals显示文摘The bleaching effect, i.e. the crystal shows that decoloration after it is illuminated by ultraviolet light, has been observed in congruent LiNbO3:Fe:Cu crystals. Based on this bleaching effect, a new technique including the recording phase by two interfering red beams and fixing phase by both UV light and a coherent red beam has been experimentally investigated to realize nonvolatile holographic storage in LiNbO3:Fe:Cu. The results of proof-of-concept experiments confirm that bleaching effect becomes an alternative physical mechanism for nonvolatile holographic storage with high recording sensitivity and weak light-induced scattering noise. | LIU Dean, LIU Liren, ZHOU Changhe, REN Liyong & LI GuangaoShanghai Institute of Optics and Fine Mechanics, Chinese Academy ofSciences, Shanghai 201800, ChinaDepartment of Physics, Nankai University, Tianjin 300071, China | 2002 | Chinese Science Bulletin2002，47，20： | 3 |
| 9 | A roughness parameter considering joint material properties and peak shear strength model for rock joints显示文摘This study aims at proposing a reasonable roughness parameter that can reflect the peak shear strength(PSS)of rock joints.Firstly,the contribution of the asperities with different apparent dip angles to shear strength is studied.Then the shear strength of the entire joint asperities is derived.The results showed that the PSS of the entire joint asperities is proportional to a key parameter hs,which is related to the geometric character of the joint surface and the joint material properties.The parameter hsis taken as the new roughness parameter,and it is reasonable to associate the PSS with the geometric characteristics of the joint surface.Based on the new roughness parameter and shear test results of 20 sets of joint specimens,a new PSS model for rock joints is proposed.The new model is validated with the artificial joints in this paper and real rock joints in published studies.Results showed that it is suitable for different types of rock joints except for gneiss joints.The new model has the form of the Mohr-Coulomb model,which can directly reflect the relationship between the 3 D roughness parameters and the peak dilation angle. | Liren Ban Weisheng Du Tianwei Jin Chengzhi Qi Xiaozhao Li | 2021 | International Journal of Mining Science and Technology2021，31，3： | 3 |
| 10 | 对可切除的中/低位直肠癌是/否进行术前放化疗的全直肠系膜切除术研究:一项前瞻性、单中心、随机试验的长期分析显示文摘背景与目的我们的Ⅱ期随机试验的初步研究结果显示,对进行全直肠系膜切除术(total mesorectal excision,TME)的患者是/否进行术前同步放化疗(concurrent chemoradiotherapy,CCRT),二者的功能性括约肌保留率和短期生存结局相近。在经过中位71个月的随访后,现报告这一长期试验的结果。方法在2008年3月23日至2012年8月2日期间,192例患有T3–T4或淋巴结阳性、可切除的中/低直肠腺癌患者被随机分为接受或不接受术前CCRT组,之后均进行TME。评估以下终点:局部复发和远处转移的累积率、无病生存(disease?free survival,DFS)和总生存(overall survival,OS)。结果对入组的184例患者资料进行分析,其中TME组有94例,CCRT+TME组有90例。在整个队列中,5年DFS和OS率分别为84.8%和85.1%。CCRT+TME组的5年DFS率为85.2%,TME组为84.3%(P=0.969);CCRT+TME组的5年OS率为83.5%,TME组为86.5%(P=0.719)。在CCRT+TME和TME组中,局部复发的5年累积率分别为6.3%和5.0%(P=0.681),远处转移的5年累积率分别为15.0%和15.7%(P=0.881)。通过亚组分析,接受CCRT未观察到5年DFS和OS的显著改善。结论两种治疗方案的长期结局相近。因此,如果可以进行高质量的TME手术和强化化疗,建议选择性地对直肠癌患者进行术前CCRT。 | Fulong Wang Wenhua Fan Jianhong Peng Zhenhai Lu Zhizhong Pan Liren Li Yuanhong Gao Hui Li Gong Chen Xiaojun Wu Peirong Ding Zhifan Zeng Desen Wan | 2019 | 癌症2019，38，5： | 2 |
| 11 | Nature:研究鉴定出侵袭性胰腺癌细胞及其弱点显示文摘在一项新的研究中,来自美国德州大学MD安德森癌症中心的研究人员利用患者源性肿瘤异种移植(patient—derived tumorxenograft,PDx)和模式小鼠开展一系列临床前实验,鉴定出一种门卫蛋白(gatekee Perprotein)阻止胰腺癌细胞转化为一种极具侵袭性的细胞类型,并且也发现剔除这种门卫蛋白的疗法能够阻止这些癌细胞。这些发现有助为携带这种进展迅速的治疗抵抗性的胰腺癌细胞亚群的患者开发出潜在的疗法。 | Genovese, Giannicola Carugo, Alessandro Tepper, James Robinson, Frederick Scott Li, Liren Svelto, Maria Nezi, Luigi Corti, Denise Minelli, Rosalba Pettazzoni, Piergiorgio Gutschner, Tony Wu, Chia-Chin Seth, Sahil Akdemir, Kadir Caner Leo, Elisabetta Amin, Samirkumar Dal Molin, Marco Ying, Haoqiang Kwong, Lawrence N. Colla, Simona Takahashi, Koichi Ghosh, Papia Giuliani, Virginia Muller, Florian Dey, Prasenjit Jiang, Shan Garvey, Jill Liu, Chang-Gong Zhang, Jianhua Heffernan, Timothy P. Toniatti, Carlo Fleming, Jason B. Goggins, Michael G. Wood, Laura D. Sgambato, Alessandro Agaimy, Abbas Maitra, Anirban Roberts, Charles W. M. Wang, Huamin Viale, Andrea DePinho, Ronald A. Draetta, Giulio F. Chin, Lynda | 2017 | 现代生物医学进展2017，17，14： | 2 |
| 12 | Electrospinning Engineering Enables High‑Performance Sodium‑Ion Batteries显示文摘As a promising energy storage device,sodium-ion batteries(SIBs)have received continuous attention due to their low-cost and environmental friendliness.However,the sluggish kinetics of Na ion usually makes SIBs hard to realize desirable electrochemical performance when compared to lithium-ion batteries(LIBs).The key to addressing this issue is to build up nanostructured materials which enable fast Na-ion insertion/extraction.One-dimensional(1D)nanocarbons have been considered as both the anode and the matrix to support active materials for SIB electrodes owing to their high electronic conductivity and excellent mechanical property.Because of their large surface areas and short ion/electron difusion path,the synthesized electrodes can show good rate performance and cyclic stability during the charge/discharge processes.Electrospinning is a simple synthetic technology,featuring inexpensiveness,easy operation and scalable production,and has been largely used to fabricate 1D nanostructured composites.In this review,we frst give a simple description of the electrospinning principle and its capability to construct desired nanostructures with diferent compositions.Then,we discuss recent developments of carbon-based hybrids with desired structural and compositional characteristics as the electrodes by electrospinning engineering for SIBs.Finally,we identify future research directions to realize more breakthroughs on electrospun electrodes for SIBs. | Chuanping Li Min Qiu Ruiling Li Xuan Li Manxi Wang Jiabo He Ganggang Lin Liren Xiao Qingrong Qian Qinghua Chen Junxiong Wu Xiaoyan Li Yiu‑Wing Mai Yuming Chen | 2022 | Advanced Fiber Materials2022，4，1： | 1 |
| 13 | Optical complex matrixvector multiplication with negative binary inner products 显示文摘 | Liu Liren Li Guoqiang Yin Yaozu | 1994 | Optics Letters1994，19，21： | 1 |
| 14 | IPv6 traffic with mutil - class QoS in VPN显示文摘 | Liren Zhang Li Zheng | 2001 | Computer Network2001，37，： | 1 |
| 15 | 5Zn-2RE alloy显示文摘 | Tingqu Li Yonghing Liu Zhanyi Cao Dongmei Jiang Liren Cheng The tensile properties and high cyclicfatigue characteristics of Mg-5Li-3Al-1 | 2010 | Materials Science and Engineering2010，，527： | 1 |
| 16 | On the aggregation kinetics of two particles trapped in an optical tweezers显示文摘 | Xu Shenghua Lou Liren Li Yinmei | 2005 | Colloids and Surfaces A:Physicochem Eng Aspects2005，255，1： | 1 |
| 17 | Web Services interface of SSRF archive data analysis system显示文摘Accelerator database stores various static parameters and real-time data of accelerator. SSRF (Shanghai Synchrotron Radiation Facility) adopts relational database to save the data. We developed a data retrieval system based on XML Web Services for accessing the archive data. It includes a bottom layer interface and an interface applicable for accelerator physics. Client samples exemplifying how to consume the interface are given. The users can browse, retrieve and plot data by the client samples. Also, we give a method to test its stability. The test result and performance are described. | LI Lin SHEN Liren ZHU Qing WAN Tianmin | 2007 | Nuclear Science and Techniques2007，18，4： | 1 |
| 18 | Enantioseparation of novel chiral tetrahedrane-type clusters on an amylose tris (Phenylcarbamate) chiral stationary phase显示文摘 | Han Xiaoqian Liu Yueqi Zhang Yuhua Zhang Weiqiang Li Yongmin Chen Liren | 2002 | Chromatographia (-)2002，，5： | 1 |
| 19 | Research on a scanner for tilting orthogonal double prisms显示文摘 | LI Anhu LIU Liren SUN Jianfeng | 2006 | Applied Optics2006，45，31： | 1 |
| 20 | Laser-induced tobacco protoplast fusion显示文摘 | Li Yinmei Guan Lijie Lou Liren | 1999 | SCIENCE IN CHINA( Seires C)1999，42，2： | 1 |
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