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| 1 | 一种高效便捷的水稻DNA提取法及其应用显示文摘以水稻叶片、根和种子为材料,采用高通量快速法提取基因组DNA,用稻瘟病Pita基因分子标记进行检测,获得与预期片段大小一致的特异性条带,对165份育种材料的检测结果与稻瘟病接种鉴定一致。操作方法如下:将少量水稻叶片、根或种子放入200μL PCR盘中,加入70μL缓冲液A(含NaOH和Tween20),在PCR仪中加热到95℃,保持10min,再加入70μL缓冲液B(Tris-HCl和EDTA),该提取液可以直接用于PCR扩增。该方法具有几个优点:1)成本低,仅用4种化学试剂,共140μL提取液;2)操作简便,仅需3步,每人每天可以提取上千份样品;3)仪器设备简单,只用常规的PCR仪;4)可直接提取干种子的DNA;5)DNA质量好,能检测出水稻中的抗稻瘟病单基因Pita,并且与稻瘟病接种鉴定结果一致;6)用量少,只需要5~20mg叶片、20mg根或半粒籽粒。尤其是检测大量样本的基因型时,此种方法更显得高效便捷。 | 赵国珍 贾育林 严宗卜 Christopher W DEREN Melissa H JIA 戴陆园 | 2012 | 中国水稻科学2012，26，4： | 21 |
| 2 | 中国糖尿病:流行病学、基因易感性和个性化治疗的临床应用显示文摘近些年来,中国2型糖尿病患病率出现快速增长,这使得2型糖尿病已经成为了一个重要的公共卫生问题。和欧洲人相比,相中国2型糖尿病诊断患者年龄较小、且体重指数较低。为了今后预防和干预项目的实施,有必要对此现象进行深入研究。除环境因素外,基因对于2型糖尿病的发生和发展起到至关重要的作用。迄今为止已经被识别的2型糖尿病的易感基因位点达百余个。就个体而言,大多数2型糖尿病的基因变异效应量较小(每个危险等位基因增加10%~20%的2型糖尿病风险)。可以利用多个等位基因来计算遗传风险得分,进而预测2型糖尿病和识别高危人群。此外,预防2型糖尿病的并发症和死亡时应优先考虑个性化的药物治疗。本研究回顾了2型糖尿病基因病因学近期的流行病学研究趋势和进展情况,并对2型糖尿病个性化治疗进行了深入探讨。 | Hu C Jia W 姜莹莹(编译) | 2019 | 中华预防医学杂志2019，53，2： | 18 |
| 3 | 冠状动脉内影像学临床应用专家共识(第一部分):对冠状动脉介入治疗的指导与优化显示文摘欧洲心血管介入协会(EAPCI)专家组系统总结了血管内超声(IVUS)和光学相干断层成像(OCT)这两种血管内影像学检查临床应用指征的现有证据,提供了关于IVUS和OCT指导经皮冠状动脉介入治疗(PCI)的应用价值,并明确了最可能从腔内影像学指导的介入治疗中获得临床收益的患者或病变类型,同时详细论述了PCI前如何使用IVUS或OCT优化支架尺寸(支架长度和直径)和手术策略的选择。此外,专家推荐对支架失败(支架内再狭窄或支架内血栓形成)的患者应常规进行冠状动脉内影像学检查,并首选OCT。最后,重点论述了IVUS和OCT在指导PCI和评估支架失败两个方面的优势和局限性,并对未来需要深入研究的领域进行了展望。 | Lorenz Raber Gary S Mintz Konstantinos C Koskinas Thomas W Johnson Niels R Holm Yoshinubo Onuma Maria D Radu Michael Joner Bo Yu Haibo Jia Nicolas Meneveau Jose M.de la Torre Hemandez Javier Escaned Jonathan Hill Francesco Prati Antonio Colombo Carlo di Mario Evelyn Regar Davide Capodanno William Wijns Robert A Byme Giulio Guagliumi | 2019 | 中华心血管病杂志2019，47，1： | 17 |
| 4 | Dietary advanced glycation end-products aggravate non-alcoholic fatty liver disease显示文摘AIM To determine if manipulation of dietary advanced glycation end product(AGE), intake affects nonalcoholic fatty liver disease(NAFLD) progression and whether these effects are mediated via RAGE. METHODS Male C57Bl6 mice were fed a high fat, high fructose, high cholesterol(HFHC) diet for 33 wk and compared with animals on normal chow. A third group were given a HFHC diet that was high in AGEs. Another group was given a HFHC diet that was marinated in vinegar to prevent the formation of AGEs. In a second experiment, RAGE KO animals were fed a HFHC diet or a high AGE HFHC diet and compared with wildtype controls. Hepatic biochemistry, histology, picrosirius red morphometry and hepatic mR NA were determined. RESULTS Long-term consumption of the HFHC diet generated significant steatohepatitis and fibrosis after 33 wk. In this model, hepatic 4-hydroxynonenal content(a marker of chronic oxidative stress), hepatocyte ballooning, picrosirius red staining, α-smooth muscle actin and collagen type 1A gene expression were all significantly increased. Increasing the AGE content of the HFHC diet by baking further increased these markers of liver damage, but this was abrogated by pre-marination in acetic acid. In response to the HFHC diet, RAGE-/-animals developed NASH of similar severity to RAGE+/+ animals but were protected from the additional harmful effects of the high AGE containing diet. Studies in isolated Kupffer cells showed that AGEs increase cell proliferation and oxidative stress, providing a likely mechanism through which these compounds contribute to liver injury. CONCLUSION In the HFHC model of NAFLD, manipulation of dietary AGEs modulates liver injury, inflammation, and liver fibrosis via a RAGE dependent pathway. This suggests that pharmacological and dietary strategies targeting the AGE/RAGE pathway could slow the progression of NAFLD. | Christopher Leung Chandana B Herath Zhiyuan Jia Sof Andrikopoulos Bronwyn E Brown Michael J Davies Leni R Rivera John B Furness Josephine M Forbes Peter W Angus | 2016 | World Journal of Gastroenterology2016，22，35： | 7 |
| 5 | Epidemiological serosurvey of Hepatitis B in China—Declining HBV prevalence due to Hepatitis B vaccination显示文摘 | Xiaofeng Liang Shengli Bi Weizhong Yang Longde Wang Gang Cui Fuqiang Cui Yong Zhang Jianhua Liu Xiaohong Gong Yuansheng Chen Fuzhen Wang Hui Zheng Feng Wang Jing Guo Zhiyuan Jia Jingchen Ma Huaqing Wang Huiming Luo Li Li Shuigao Jin Stephen C. Hadler Yu W | 2009 | Vaccine2009，，47： | 7 |
| 6 | The structural basis of the dominant negative phenotype of the Gαi1β1Y2 G203A/A326S heterotrimer显示文摘 | Ping LIU Ming-zhu JIA X Edward ZHOU Parker W DE WAAL Bradley M DICKSON Bo LlU Li HOU Yan-ting YIN Ya n-yong KANG Yi SHI Karsten MELCHER H Eric XU Yi JIANG | 2016 | Acta Pharmacologica Sinica2016，37，9： | 3 |
| 7 | The genetic heterozygosity and fitness of tetraploid embryos and embryonic stem cells are crucial parameters influencing survival of mice derived from embryonic stem cells by tetraploid embryo aggregation显示文摘 | Li X Wei W Yong J Jia Q Yu Y Di K | 2006 | 中国生物学文摘2006，20，7： | 3 |
| 8 | Magnetoelectric and converse magnetoelectric responses in Tb_xDy_(1-x)Fe_(2-y) alloy & Pb(Mg_(1/3)Nb_(2/3))_(1-x)Ti_xO_3 crystal laminated composites显示文摘Measured results of magnetoelectric (ME) and converse magnetoelectric (CME) effects of TbxDy1-xFe2-y/ Pb(Mg1/3Nb2/3)(1-x)TixO3/TbxDy1-xFe2-y (TD/PMNT/TD) and PMNT/TD/PMNT laminated composites are pre- sented. ME effect was determined by measuring laminate voltage output under a Helmholtz-gener- ated AC field biased by a DC field (0―1 kOe) (1 Oe = 79.58 A/m). The CME effect was measured by re- cording the voltage induced in a solenoid encompassing the ME sample while exposed to a DC bias field and PMNT layer driven by a 10 V AC source. The ME and CME responses in the two laminated structure are linear. The highest values of ME coefficients in TD/PMNT/TD and PMNT/TD/PMNT com- posites are 384 mV/Oe and 158 mV/Oe, respectively, while the highest values of CME coefficients in the two composites are 118 mG/V and 162 mG/V (1 G=10-4 T), respectively. | JIA YanMin LUO HaoSu OR Siu Wing WANG YaoJin CHAN Helen L W | 2008 | Chinese Science Bulletin2008，53，14： | 3 |
| 9 | Study on the suppression effect of matural zeolite on expansion of concrete due to AAR显示文摘 | FENG N Q JIA H W CHEN E Y | 1998 | Mag Concr Res1998，50，1： | 2 |
| 10 | 显示文摘 | Jia W Y Yuan H B Lu L Z | 1998 | Journal of Luminescence1998，7677，： | 1 |
| 11 | Effects and mechanisms of total glu- cosides of paeony on adjuvant arthritis in rats 显示文摘 | Xu HM Wei W Jia XY | 2007 | Ethnopharmacol2007，109，3： | 1 |
| 12 | Diamond Formation from a System of SiC and a Metal显示文摘 | Hong S M Li W Jia X P | 1993 | Diamond Related Mater1993，2，： | 1 |
| 13 | Electrocatalytic properties of platinum on hard carbon spherules derived from deoiled asphalt for methanol oxidation显示文摘 | Xu B S Guo J J Jia H S Yang X W Liu X G | 2007 | Catalysis Today2007，125，34： | 1 |
| 14 | Development and characterization of a new tropical marine fish cell line from grouper, Epinephelus coioides susceptible to iridovirus and nodavirus 显示文摘 | Qin Q W Wu T H Jia T L | 2006 | J Virol Methods2006，131，1： | 1 |
| 15 | Location of the ground state of Er3+in doped Y2O3 from two-step photoconductivity显示文摘 | Jia D D Meltzer R S Yen W M 2002 | 2002 | Phys Rev B2002，65，23： | 1 |
| 16 | Relationship of kernel moisture content gradients and glass transition temperatures to head rice yield显示文摘 | YANG W JIA C C SIEBENMORGEN T J | 2003 | Biosystems Eng2003，85，4： | 1 |
| 17 | Synthesis and antibacterial activities of quaternary ammonium salt of chitosan显示文摘 | Jia Z S Shen D F Xu W L | 2001 | Carbohydrate Res2001，333，1： | 1 |
| 18 | Management of Chinese patients with type 2 diabetes, 1998-2006: the Diabeare-China surveys显示文摘 | Pan C Yang W Jia W | 2008 | Current Medical Research and Oplmon2008，25，1： | 1 |
| 19 | Paeonol attenuates high - fat - diet - induced atherosclerosis in rabbits by anti - inflammatory activity 显示文摘 | Li H Dai M Jia W | 2009 | Plan- ta Med2009，75，1： | 1 |
| 20 | A 100 kW high-per- formance PWM rectifier with a ZCT soft-switching tech- nique显示文摘 | Jia W Lee F C Boroyevich D | 2003 | IEEE Transactions on Power Electronics2003，18，6： | 1 |
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