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| 1 | Evolution of the novel coronavirus from the ongoing Wuhan outbreak and modeling of its spike protein for risk of human transmission显示文摘Dear Editor,The occurrence of concentrated pneumonia cases in Wuhan city,Hubei province of China was first reported on December 30,2019 by the Wuhan Municipal Health Commission(WHO,2020).The pneumonia cases were found to be linked to a large seafood and animal market in Wuhan,and measures for sanitation and disinfection were taken swiftly by the local government agency.The Centers for Disease Control and Prevention(CDC)and Chinese health authorities later determined and announced that a novel coronavirus(CoV),denoted as 2019-nCoV,had caused the pneumonia outbreak in Wuhan city(CDC,2020).Scientists from multiple groups had obtained the virus samples from hospitalized patients(Normile,2020).The isolated viruses were morphologically identical when observed under electron microscopy. | Xintian Xu Ping Chen Jingfang Wang Jiannan Feng Hui Zhou Xuan Li Wu Zhong Pei Hao | 2020 | Science China(Life Sciences)2020，63，3： | 737 |
| 2 | High expression of ACE2 receptor of 2019-nCoV on the epithelial cells of oral mucosa显示文摘It has been reported that ACE2 is the main host cell receptor of 2019-nCoV and plays a crucial role in the entry of virus into the cell to cause the final infection. To investigate the potential route of 2019-n Cov infection on the mucosa of oral cavity, bulk RNA-seq profiles from two public databases including The Cancer Genome Atlas(TCGA) and Functional Annotation of The Mammalian Genome Cap Analysis of Gene Expression(FANTOM5 CAGE) dataset were collected. RNA-seq profiling data of 13 organ types with para-carcinoma normal tissues from TCGA and 14 organ types with normal tissues from FANTOM5 CAGE were analyzed in order to explore and validate the expression of ACE2 on the mucosa of oral cavity. Further, single-cell transcriptomes from an independent data generated in-house were used to identify and confirm the ACE2-expressing cell composition and proportion in oral cavity. The results demonstrated that the ACE2 expressed on the mucosa of oral cavity. Interestingly, this receptor was highly enriched in epithelial cells of tongue. Preliminarily, those findings have explained the basic mechanism that the oral cavity is a potentially high risk for 2019-nCoV infectious susceptibility and provided a piece of evidence for the future prevention strategy in dental clinical practice as well as daily life. | Hao Xu Liang Zhong Jiaxin Deng Jiakuan Peng Hongxia Dan Xin Zeng Taiwen Li Qianming Chen | 2020 | International Journal of Oral Science2020，12，1： | 69 |
| 3 | Effect of Surface Mechanical Attrition Treatment on Corrosion Behavior of 316 Stainless Steel显示文摘The nanocrystalline microstructure of the surface of 316 stainless steel (316SS) induced by surface mechanical attrition treatment (SMAT) was determined by X-ray diffraction (XRD) and scanning electron microscopy (SEM). The technique of hydrogen embrittlement was first used to obtain the information of the brittleness cleavage plane. The effects of SMAT and the following annealing process on the corrosion behavior of 316SS were investigated by potentiodynamic polarization curves and potentiostatic critical pitting temperature measurements. The results demonstrated that the nanocrystalline layer with an average grain size of 19 nm was produced. However, there were lots of cracks on the surface, which led to the degradation in the corrosion resistance of 316SS after SMAT. Nevertheless, after annealing treatment, the corrosion resistance of the nanocrystalline surface had been improved greatly. The higher the annealing temperature, the better was the corrosion resistance. | HAO Yun-wei DENG Bo ZHONG Cheng JIANG Yi-ming LI Jin | 2009 | Journal of Iron and Steel Research(International)2009，16，2： | 26 |
| 4 | Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity显示文摘感觉神经原被不同发信号的网络和接受特征区分。因此,感觉神经原类型能被连接基于 transcriptome 的神经原与感觉显型打字定义。这里,我们分类老鼠的 somatosensory 神经原由高范围的单个房间的定序 RNA 的背面的根中心(DRG )(10 950 ±1 218 基因每神经原) 并且神经原基于尺寸的层次聚类。而且,对皮肤的刺激作出回应的单个 DRG 神经原被记录用一在里面 vivo 整个房间的补丁夹钳技术并且由神经原类型分类基因标记。小直径 DRG 神经原被分类进低阀值的 mechanoreceptor 的一种类型和 mechanoheat nociceptors (MHN ) 的五种类型。每 MHN 类型进一步被分成二种子类型。大 DRG 神经原被分成四种类型,包括表示联系 BAI1 的蛋白质 2-like 的 neurexophilin 1 快车 MHN 和机械 nociceptors (MN ) 1 (Baiap2l1 ) 。表示 trafficking 蛋白质粒子建筑群 3-like 和 Baiap2l1 显著的 MN 的 Mechanoreceptors 各被细分进二种子类型。这些结果为编目 somatosensory 神经原和他们的 transcriptome 数据库提供一个新系统。 | Chang-Lin Li Kai-Cheng Li Dan Wu Yan Chen Hao Luo Jing-Rong Zhao Sa-Shuang Wang Ming-Ming Sun Ying-Jin Lu Yan-Qing Zhong Xu-Ye Hu Rui Hou Bei-Bei Zhou Lan Bao Hua-Sheng Xiao Xu Zhang | 2016 | Cell Research2016，26，1： | 21 |
| 5 | The Role of Fibrocyte in the Pathogenesis of Silicosis显示文摘Exposure to free silica induces silicosis and myofibroblasts are regarded as primary effector cells.Fibrocytes can differentiate into myofibroblast.Therefore,the present study was designed to investigate whether | LI Juan YAO Wu HOU Jian Yong ZHANG Lin BAO Lei CHEN Hui Ting WANG Di YUE Zhong Zheng LI Yi Ping ZHANG Miao YU Xing Hao ZHANG Jian Hui QU Ya Qian HAO Chang Fu | 2018 | Biomedical and Environmental Sciences2018，31，4： | 20 |
| 6 | β-sitosterol ameliorates influenza A virus-induced proinflammatory response and acute lung injury in mice by disrupting the cross-talk between RIG-I and IFN/STAT signaling显示文摘β-Sitosterol(24-ethyl-5-cholestene-3-ol)is a common phytosterol Chinese medical plants that has been shown to possess antioxidant and anti-inflammatory activity.In this study we investigated the effects ofβ-sitosterol on influenza virus-induced inflammation and acute lung injury and the molecular mechanisms.We demonstrate thatβ-sitosterol(150–450μg/mL)dosedependently suppresses inflammatory response through NF-κB and p38 mitogen-activated protein kinase(MAPK)signaling in influenza A virus(IAV)-infected cells,which was accompanied by decreased induction of interferons(IFNs)(including Type I and III IFN).Furthermore,we revealed that the anti-inflammatory effect ofβ-sitosterol resulted from its inhibitory effect on retinoic acidinducible gene I(RIG-I)signaling,led to decreased STAT1 signaling,thus affecting the transcriptional activity of ISGF3(interferonstimulated gene factor 3)complexes and resulting in abrogation of the IAV-induced proinflammatory amplification effect in IFNsensitized cells.Moreover,β-sitosterol treatment attenuated RIG-I-mediated apoptotic injury of alveolar epithelial cells(AEC)via downregulation of pro-apoptotic factors.In a mouse model of influenza,pre-administration ofβ-sitosterol(50,200 mg·kg−1·d−1,i.g.,for 2 days)dose-dependently ameliorated IAV-mediated recruitment of pathogenic cytotoxic T cells and immune dysregulation.In addition,pre-administration ofβ-sitosterol protected mice from lethal IAV infection.Our data suggest thatβ-sitosterol blocks the immune response mediated by RIG-I signaling and deleterious IFN production,providing a potential benefit for the treatment of influenza. | Bei-xian Zhou Jing Li Xiao-li Liang Xi-ping Pan Yan-bing Hao Pei-fang Xie Hai-ming Jiang Zi-feng Yang Nan-shan Zhong | 2020 | Acta Pharmacologica Sinica2020，41，9： | 20 |
| 7 | Status quo of soil petroleum contamination and evolution of bioremediation显示文摘Along with the rapid development of oil industries internationally,petroleum prospecting and exploitation activities are growing intensively.Especially in China,with the fastest economic growth in the world and shortage of petroleum resources,we are leading the practices of petroleum deep exploitation.Obviously,the risk of damage to the natural environment from these activities is high.Oil contamination in soils and groundwater is becoming a big issue along with pesticide pollution,which makes organic pollution prevention and control (OPPC) much more complex.In this paper,based on recent research on oil-contaminated soil at home and abroad,we make comments on the remediation technologies for polluted soil,emphasizing bioremediation techniques and degradation mechanisms in order to push forward research into bound organic pollution prevention and control (OPPC),especially in China. | Du Weidong Wan Yunyang Zhong Ningning Fei Jiajia ZhangZhihuan Chen Lijun Hao Jiming | 2011 | Petroleum Science2011，8，4： | 19 |
| 8 | Effectiveness of Different Waist Circumference Cut-off Values in Predicting Metabolic Syndrome Prevalence and Risk Factors in Adults in China显示文摘Objective To study the effectiveness of waist circumference cut-off values in predicting the prevalence of metabolic syndrome(MetS) and risk factors in adults in China. Methods A cross-sectional survey was condcuted in 14 provinces(autonomous region, municipality) in China. A total of 47 325 adults aged ≥20 years were selected by multistage stratified sampling, and questionnaire survey and physical and clinical examination were conducted among them. MetS was defined according to the International Diabetes Federation(IDF) criteria and modified IDF criteria.Results The age-standardized prevalence of MetS was 24.2%(22.1% in men and 25.8% in women) and 19.5%(22.1% in men and 18.0% in women) according to the IDF criteria and modified IDF criteria respectively. The age-standardized prevalence of pre-MetS was 8.1%(8.6% in men and 7.8% in women) according to the modified IDF criteria. The prevalence of MetS was higher in urban residents than rural residents and in northern China residents than in southern China residents. The prevalence of central obesity was about 30% in both men and women according to the ethnicity-specific cut-off values of waist circumference for central obesity(90 cm for men and 85 cm for women). Multivariate regression analysis revealed no significant difference in risk factors between the two MetS definitions. Conclusion Using both the modified IDF criteria and ethnicity-specific cut-off values of waist circumference can provide more useful information about the prevalence of MetS in China. | ZHOU Hai Cheng LAI Ya Xin SHAN Zhong Yan JIA Wei Ping YANG Wen Ying LU Ju Ming WENG Jian Ping JI Li Nong LIU Jie TIAN Hao Ming JI Qiu He ZHU Da Long CHEN Li GUO Xiao Hui ZHAO Zhi Gang Li Qiang ZHOU Zhi Guang GE Jia Pu SHAN Guang Liang | 2014 | Biomedical and Environmental Sciences2014，27，5： | 17 |
| 9 | Numerical simulation of powder transport behavior in laser cladding with coaxial powder feeding显示文摘Laser cladding with coaxial powder feeding is one of the new processes applied to produce well bonding coating on the component to improve performance of its surface. In the process, the clad material is transported by the carrying gas through the coaxial nozzle, generating gas-powder flow. The powder feeding process in the coaxial laser cladding has important influence on the clad qualities. A 3D numerical model was developed to study the powder stream structure of a coaxial feeding nozzle. The predicted powder stream structure was well agreed with the experimental one. The validated model was used to explore the collision behavior of particles in the coaxial nozzle, as well as powder concentration distribution. It was found that the particle diameter and restitution coefficient greatly affect the velocity vector at outlet of nozzle due to the collisions, as well as the powder stream convergence characteristics below the nozzle. The results indicated a practical approach to optimize the powder stream for the coaxial laser cladding. | LIU Hao HE XiuLi YU Gang WANG Zhong Bin LI ShaoXia ZHENG CaiYun NING WeiJian | 2015 | Science China(Physics,Mechanics & Astronomy)2015，58，10： | 17 |
| 10 | Effects of simulated carbon dioxide and helium peumoperitoneum on proliferation and apoptosis of gastric cancer cells显示文摘AIM:To investigate the effects of carbon dioxide (CO2) and helium insufflation administered at different pressures on the growth and apoptosis of cultured human gastric cancer cells. METHODS:The gastric cancer cells MKN-45 were exposed to a CO2 and helium environment maintained at different pressures (0, 5, 10 and 15 mmHg). The cells were exposed to simulated pneumoperitoneum environment for 4 h, and pH of the culture media was measured after it was moved to normal conditions for 0, 2, 4, 6 and 8 h. Proliferation viability of MKN-45 was examined by 3-4,5Dimethylthiazol-2-yl,5-diphenyltetrazolium bromide or triazolyl blue (MTT) assay after it was moved to normal conditions. Apoptotic ratio was measured by Annexin V-FITC/PI double labelled staining. RESULTS:The pH of media was acid and recovered to normal after 4 h in the CO2 group while it was basic in the helium group. There was no difference between CO2 groups (under 10 mmHg ) and control group (P > 0.05) in the proliferative viability of the cells. The cultured cells exposed to 15 mmHg CO2 environment grew more slowly than control group from 4 to 7 d (P < 0.01 ) while there was no difference from 1 to 3 d (P > 0.05). The proliferative viability in helium group was not obviously different from the control group (P > 0.05). The apoptotic ratio of the cultured cells was markedly higher than that of the control group (P < 0.01) at 10 and 15 mmHg CO2 insufflation pressure. In helium group, the apoptotic ratio was not obviously different from the control group (P > 0.05). CONCLUSION:There is no obvious effect in the proliferation and apoptosis of MKN-45 cells under 10 mmHg CO2 insufflation pressure and helium in any pressure. Fifteen mmHg CO2 insufflation pressure can inhibit the proliferation of the cells and improve apoptosis. | Ying-Xue Hao Hua Zhong Chao Zhang Dong-Zu Zeng Yan Shi Bo Tang Pei-Wu Yu | 2008 | World Journal of Gastroenterology2008，14，14： | 16 |
| 11 | Prevalence, awareness, treatment, and control of hypertension in the non-dialysis chronic kidney disease patients显示文摘 | ZHENG Ying CAI Guang-yan CHEN Xiang-mei FU Ping CHEN Jiang-hua DING Xiao-qiang YU Xue-qing LIN Hong-li LIU Jian XIE Ru-juan WANG Li-ning NI Zhao-hui LIU Fu-you YIN Ai-ping XING Chang-ying WANG Li SHI Wei LIU Jian-she HE Ya-ni DING Guo-hua LI Wen-ge WU Guang-li MIAO Li-ning CHEN Nan SU Zhen MEI Chang-lin ZHAO Jiu-yang GU Yong BAI Yun-kai LUO Hui-min LIN Shan CHEN Meng-hua GONG Li YANG Yi-bin YANG Xiao-ping LI Ying WAN Jian-xin WANG Nian-song LI Hai-ying XI Chun-sheng HAO Li XU Yan FANG Jing-ai LIU Bi-cheng LI Rong-shan WANG Rong ZHANG Jing-hong WANG Jian-qin LOU Tan-qi SHAO Feng-min MEI Feng LIU Zhi-hong YUAN Wei-jie SUN Shi-ren ZHANG Ling ZHOU Chun-hua CHEN Qin-kai JIA Shun-lian GONG Zhi-feng GUAN Guang-ju XIA Tian ZHONG Liang-bao | 2013 | Chinese Medical Journal2013，，12： | 16 |
| 12 | Molecular Epidemiological Characteristics of Streptococcus pyogenes Strains Involved in an Outbreak of Scarlet Fever in China,2011显示文摘Objective To investigate molecular characterization of streptococcus pyogenes isolates involved in an outbreak of scarlet fever in China in 2011.Methods Seventy-four Streptococcal pyogenes involved in an outbreak of scarlet fever were isolated from pediatric patients in the areas with high incidence in China from May to August of 2011.Emm genotyping,pulsed-field gel electrophoresis (PFGE),superantigen (SAg) genes and antimicrobial susceptibility profiling were analyzed for these isolates.Results A total of 4 different emm types were identified.Emm12 was the most prevalent type which contained four predominating PFGE patterns corresponding to four different virulence and superantigen profiles.Emm12 (79.7%) and emml (14.9%) accounted for approximately 94%of all the isolates.The speA gene was all negative in emm12 isolates and positive in emml isolates.All strains were resistant to erythromycin,and 89.4%of them were resistant to erythromycin,tracycline,and clindamycin simultaneously.Conclusion Several highly diversified clones with a high macrolide resistance rate comprise a predominant proportion of circulating strains,though no new emm type was found in this outbreak.The data provide a baseline for further surveillance of scarlet fever,which may contribute to the explanation of the outbreak and development of a GAS vaccine in China. | YOU Yuan Hai SONG Yan Yan YAN Xiao Mei WANG Hai Bin ZHANG Meng Han TAO Xiao Xia Li Lei Lei ZHANG Yu Xin JIANG Xi Hong ZHANG Bing Hua ZHOU Hao XIAO Di JIN Lian Mei FENG Zi Jian LUO Feng Ji ZHANG Jian Zhong | 2013 | Biomedical and Environmental Sciences2013，26，11： | 15 |
| 13 | Biphasic effects of sodium danshensu on vesse function in isolated rat aorta显示文摘 | Ning ZHANG Hao ZOU Lei JIN Jian WANG Mei-fang ZHONG Peng HUANG Bing-qing GU Shi-long MAO Chuan ZHANG Hong CHEN | 2010 | Acta Pharmacologica Sinica2010，31，4： | 13 |
| 14 | Long-term Efficacy of Subthalamic Nucleus Deep Brain Stimulation in Parkinson's Disease: A 5-year Follow-up Study in China显示文摘 | Lu-Lu Jiang Jin-Long Liu Xiao-Li Fu Wen-Biao Xian Jing Gu Yan-Mei Liu Jing Ye Jie Chen Hao Qian Shao-Hua Xu Zhong Pei Ling Chen | 2015 | Chinese Medical Journal2015，，18： | 13 |
| 15 | Inulin Can Alleviate Metabolism Disorders in ob/ob Mice by Partially Restoring Leptinrelated Pathways Mediated by Gut Microbiota显示文摘Inulin has been used as a prebiotic to alleviate glucose and lipid metabolism disorders in mice and humans by modulating the gut microbiota. However, the mechanism underlying the alleviation of metabolic disorders by inulin through interactions between the gut microbiota and host cells is unclear. We use ob/ob mice as a model to study the effect of inulin on the cecal microbiota by16 S rRNA gene amplicon sequencing and its interaction with host cells by transcriptomics. The inulin-supplemented diet improved glucose and lipid metabolism disorder parameters in ob/ob mice,alleviating fat accumulation and glucose intolerance. The a diversity of gut microbial community of ob/ob mice was reduced after inulin treatment, while the b diversity tended to return to the level of wild type mice. Interestingly, Prevotellaceae UCG 001(family Prevotellaceae) was obviously enriched after inulin treatment. A comparative analysis of the gene expression profile showed that the cecal transcriptome was changed in leptin gene deficiency mice, whereas the inulin-supplemented diet partially reversed the changes in leptin gene-related signaling pathways, especially AMPK signaling pathway, where the levels of gene expression became comparable to those in wild type mice.Further analysis indicated that Prevotellaceae UCG 001 was positively correlated with the AMPK signaling pathway, which was negatively correlated with markers of glycolipid metabolism disorders. Our results suggest that the inulin-supplemented diet alleviates glucose and lipid metabolism disorders by partially restoring leptin related pathways mediated by gut microbiota. | Xiaofeng Song Liang Zhong Na Lyu Fei Liu Boxing Li Yanan Hao Yong Xue Jing Li Yuqing Feng Yue Ma Yongfei Hu Baoli Zhu | 2019 | Genomics, Proteomics & Bioinformatics2019，17，1： | 12 |
| 16 | Chlorogenic Acid Maintains Glucose Homeostasis through Modulating the Expression of SGLT-1,GLUT-2,and PLG in Different Intestinal Segments of Sprague-Dawley Rats Fed a High-Fat Diet显示文摘Objective To reveal the effects and related mechanisms of chlorogenic acid(CGA)on intestinal glucose homeostasis.Methods Forty male Sprague-Dawley rats were randomly and equally divided into four groups:normal chow(NC),high-fat diet(HFD),HFD with low-dose CGA(20 mg/kg,HFD-LC),and HFD with high-dose CGA(90 mg/kg,HFD-HC).The oral glucose tolerance test was performed,and fast serum insulin(FSI)was detected using an enzyme-linked immunosorbent assay.The m RNA expression levels of glucose transporters(Sglt-1 and Glut-2)and proglucagon(Plg)in different intestinal segments(the duodenum,jejunum,ileum,and colon)were analyzed using quantitative real-time polymerase chain reaction.SGLT-1 protein and the morphology of epithelial cells in the duodenum and jejunum was localized by using immunofluorescence.Results At both doses,CGA ameliorated the HFD-induced body weight gain,maintained FSI,and increased postprandial 30-min glucagon-like peptide 1 secretion.High-dose CGA inhibited the HFD-induced elevation in Sglt-1 expression.Both CGA doses normalized the HFD-induced downregulation of Glut-2 and elevated the expression of Plg in all four intestinal segments.Conclusion An HFD can cause a glucose metabolism disorder in the rat intestine and affect body glucose homeostasis.CGA can modify intestinal glucose metabolism by regulating the expression of intestinal glucose transporters and Plg,thereby controlling the levels of blood glucose and insulin to maintain glucose homeostasis. | PENG Bing Jie ZHU Qi ZHONG Ying Li XU Shi Hao WANG Zheng | 2015 | Biomedical and Environmental Sciences2015，28，12： | 12 |
| 17 | Effect of oxymatrine on specific cytotoxic T lymphocyte surface programmed death receptor-1 expression in patients with chronic hepatitis B显示文摘 | GU Xi-bing YANG Xiao-juan HUA Zhong LU Zhong-hua ZHANG Bo ZHU Yin-fang WU Hang-yuan JIANG Yi-ming CHEN Hao-kun PEI Hao | 2012 | Chinese Medical Journal2012，，8： | 11 |
| 18 | NURBS reconstruction of digital terrain for hydropower engineering based on TIN model显示文摘数字地面模型(DTM ) 在 3D 设计,视觉分析和 3D 在大规模水力发出的电力工程的地质的建模起了一个重要作用。作为 3D 可视化的枢轴的基地并且建模, DTM 应该被高精确,更少的存储和井交流在图形的操作期间描绘。考虑数据来源的差异并且利用二数据结构, triangulated 不规则的网络(锡) 和不一致的合理 B 花键(NURBS ) ,新奇方法论为重建设计水力发出的电力的地面被介绍工程。与多来源数据的集成,提高了 Delaunay 算法被介绍重建 TIN DTM,它是在锡和复杂地形学,但是在低记忆的效率的忠诚描写的地面表面。基于 TIN 模型,使用扫描采样和线性插值的节,从分离、不规则、多样的数据的转变到连续、常规的采样代表性的曲线顺序,被认识到。采样数据的适当压缩也被强加为保证下列重建工作被表现。最后,采用 NURBS 技术和 skinning 方法, NURBS DTM,它代表 NURBS 表面并且在精确以后满足要求与加权的错误估计,与中间的数据被重建。同时,有另一成就,地面数据,从采样数据的从起始的数据的数据和其它的二个数据库,为重复被建立有不同要求的有能力的重建。用介绍方法的成功的申请,一个稳定的基础为设计地质的建模,视觉设计和水力发出的电力工程的分析的 3D 被打。 | Denghua Zhong Jie Liu Mingchao Li Caiwei Hao | 2008 | Progress in Natural Science:Materials International2008，18，11： | 11 |
| 19 | A multiplex PCR for differential detection of Echinococcus granulosus sensu stricto,Echinococcus multilocularis and Echinococcus canadensis in China显示文摘Background:Echinococcosis caused byEchinococcus is one of the most major infectious diseases in north-west highland of China.E.granulosus sensu strict,E.multilocularis,andE.canadensis are known to be the only three species related to human health transmitting in the areas.To achieve targeted treatment and control of echinococcosis,the accurate identification and discrimination of the species are important.However,currently the available diagnostic approaches do not present ideal results either in accuracy or efficiency.Methods:In the study,a set of primers were designed to aim at the three human-pathogenicEchinococcus species in China.The one-step multiplex PCR assay was developed and evaluated for the specificity and sensitivity.A total of 73parasitic lesions and 41 fecal materials obtained from human and various animals collected in the clinic and the field were tested to assess the applicability of this method.Results:The multiplex PCR effectively detected the individual DNA from the targeted species and their random mixtures generating with distinguishable expected size of products.The detection limit of the assay for each of the three species was 5 pg/μl when they were tested separately.When DNA mixtures of the targeted species containing the same concentration were used as templates,the lowest amount of DNA which can be detected was 50 pg/μl,10 pg/μl and 5 pg/μl forE.granulosus s.s.,E.multilocularis,andE.canadensis respectively.No cross-reactivity was observed when DNA from eight genetically close species was used as control templates.The multiplex PCR identifications of all samples were in line with the original sequencing results except for those infected withE.shiquicus,which showed negative signals in the developed assay.Of all the tested stool materials,16 were previously found positive forEchinococcus by visual and microscopic examination.Among these 16 samples,13 were confirmed by the multiplex PCR,and the other three tested negative.Additionally,the multiplex PCR identified another 14 positive feces from the remained 25 stool samples which absence of worms.Conclusions:The developed multiplex PCR shows advantages in fast diagnosis and large-scale epidemiological investigation,which proven to be a promising tool utilized in clinic and surveillance system. | Jing-Ye Shang Guang-Jia Zhang Sha Liao Yan Huang Wen-Jie Yu Wei He Guang-You Yang Tiao-Ying Li Xing-Wang Chen Bo Zhong Qian Wang Qi Wang Rui-Rui Li Hao Wang | 2019 | Infectious Diseases of Poverty2019，8，4： | 11 |
| 20 | Expert consensus on the evaluation and diagnosis of combat injuries of the Chinese People's Liberation Army显示文摘The accurate assessment and diagnosis of combat injuries are the basis for triage and treatment of combat casualties. A consensus on the assessment and diagnosis of combat injuries was made and discussed at the second annual meeting of the Professional Committee on Disaster Medicine of the Chinese People's Liberation Army(PLA). In this consensus agreement, the massive hemorrhage, airway, respiration, circulation and hypothermia(MARCH) algorithm, which is a simple triage and rapid treatment and field triage score, was recommended to assess combat casualties during the first-aid stage, whereas the abbreviated scoring method for combat casualty and the MARCH algorithm were recommended to assess combat casualties in level Ⅱ facilities. In level Ⅲ facilities, combined measures, including a history inquiry, thorough physical examination, laboratory examination, X-ray, and ultrasound examination, were recommended for the diagnosis of combat casualties. In addition, corresponding methods were recommended for the recognition of casualties needing massive transfusions, assessment of firearm wounds, evaluation of mangled extremities, and assessment of injury severity in this consensus. | Zhao-wen Zong Lian-yang Zhang Hao Qin Si-xu Chen Lin Zhang Lei Yang Xiao-xue Li Quan-wei Bao Dao-cheng Liu Si-hao He Yue Shen Rong Zhang Yu-feng Zhao Xiao-zheng Zhong representing the PLA Professional Committee and Youth Committee on Disaster Medicine | 2018 | Military Medical Research2018，5，3： | 10 |
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