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| 1 | Inhibitory effect of IGF-Ⅱ antisense RNA on malignant phenotype of hepatocellular carcinoma显示文摘INIRODUCTIONAccording to the therapeutic effect and strategy ofantisense RNA for hepatoccllular carcinoma(HCC),we have specifically synthesized partialcDNA of human insulin-like growth factor Ⅱ(IGF-Ⅱ)and constructed IGF-Ⅱ cDNA antisenseeukaryotic expression vector.The constructedvector was introduced into hepatoma cell lineSMMC-7721 to block the intrinsic IGF-Ⅱexpression.The biological behavior changes ofhepatoma cells were observed.All these | Dong Hua Yang Ming Qing Zhang Jiang Du Chong Xu Oiao Ming Liang Ji Fang Mao Han Rong Qin Zi Rong Fan Department of Gastroenterology,Zhujiang Hospital,the First Military Medical University,Guangzhou 510282,China Laboratory of Molecular Biology,Zhujiang Hospital,the First Military Medical University,Guangzhou,China Departrnent of Biochemistry,the Second Military Medical University,Shanghai,China | 2000 | World Journal of Gastroenterology2000，6，2： | 54 |
| 2 | Effects of AT1 receptor antagonist,Iosartan,on rat hepatic fibrosis induced by CCl_4显示文摘AIM To investigate effect of losartan,an AT1receptor antagonist,on hepatic fibrosis induced byCCl;and to determine whether or not AT1receptors are expressed on hepatic stellate cells,METHODS AND RESULTS Fifty male Sprague-Dawley rats,weighing(180±20)g,wererandomized into five groups(control group,modelgroup,and three losartan treated groups),inwhich all rats were given the subcutaneousinjection of 40% CCl4(every 3 days for 6 weeks)except for rats of control group.Rats of losartan-treated groups were treated with losartan(20 mg/kg,10 mg/kg,5 mg/kg,daily gavage),After 6weeks liver tissue and serum samples of all ratswere examined.Serum hyaluronic acid(HA),procollagen typeⅢ(PCⅢ)were detected byradioimmunoassays,van Giesion collagen stainingwas used to evaluate the extracellular matrix of ratswith liver fibrosis.The expression of AT1receptors,transforming growth factor-beta(TGF-β),and alpha-smooth muscle actin(a-SMA)inliver tissue were determined byimmunohistochemical techniques.Compared withmodel group,serum ALT and AST of losartan-treated groups were significantly reduced(t=4.20,P<0.01 and t=4.57,P<0.01).Serum HAand PCⅢalso had significant differences(t=3.53,P<0.01 and t=2.20,P<0.05).Thedegree of fibrosis was improved by losartan and correlated with the expressions of AT1 receptors,TGF-β,and α-SMA in liver tissue.CONCLUSION AT1 receptor antagonist,losartan,could limit the progression of the hepatic fibrosisinduced by CCl4.The mechanism may be related tothe decrease in the expression of AT1 receptorsand TGF-β,ameliorating the injury of hepatocytes;activation of local renin-angiotensin system mightrelate to hepatic fibrosis;and during progressionof fibrosis,activated hepatic stellate cells mightexpress AT1 receptors. | Hong Shan Wei Ding Guo Li Han Ming Lu Yu Tao Zhan Zhi Rong Wang Xin Huang Jing Zhang Ji Lin Cheng Qin Fang Xu Department of Gastroenterology,Xinhua Hospital,Shanghai Second Medical University,Shanghai 200092,China | 2000 | World Journal of Gastroenterology2000，6，4： | 42 |
| 3 | Effect of hepatitis C virus infection on expression of several cancer-associated gene products in hepatocellular carcinoma显示文摘NTRODUCTIONOurpreviousstudiesbyseroepidemiological,molecularepidemiologicalandimmunopathologicalmethodshaverevealedthathepa... | YANG Jian Min, WANG Rong Quan, BU Bao Guo, ZHOU Zi Cheng, FANG Dian Chun and LUO Yuan Hui | 1999 | World Journal of Gastroenterology1999，5，1： | 42 |
| 4 | Expression and significance of proapoptotic gene Bax in gastric carcinoma显示文摘INTRODUCTIONRecentinvestigationshavedemonstratedthatapoptosisplaysasignificantroleinthepathogenesisoftumors[1,2].Emphasishas... | LIU Hai Feng, LIU Wei Wen, FANG Dian Chun and MEN Rong Pu | 1999 | World Journal of Gastroenterology1999，5，1： | 32 |
| 5 | The regulatory role of AT 1 receptor on activated HSCs in hepat,c fibrogenesis,effects of RAS inhibitors on hepatic fibrosis induced by CCl_4显示文摘AIM To assess the effect of ACE inhibitor andAng Ⅱ type Ⅰ(AT1)receptor antagonist inpreventing hepatic fibrosis caused by CCl4administration in rats;to investigate whether ornot there are expression of AT 1 receptors onhepatic stellate cells;and to observe the effectof Ang Ⅱ on proliferation and ECM synthesis ofcultured HSCs.METHODS Studies were conducted in maleSprague-Dawley rats.Except for thehepatofibrotic model group and the controlgroup,in three treated groups,either enalapril(5 mg/kg),or Iosartan(10 mg/kg),or enalapril+Iosartan were given to the fibrotic rats bydaily gavage,and saline vehicle was given tomodel and normal control rats.After 6 weeks,liver fibrosis was assessed directly by hepaticmorphometric analysis,which has beenconsidered the gold standard for thequantification of fibrosis.The expressions of AT1 receptors and(α-mooth muscle actin,α-SMA)in liver tissue or isolated hepatic stellate cells(HSCs)were detected by immunohistochemicaltechniques.The effect of Ang Ⅱ on HSCproliferation was determined by MTT method.Effect of Ang Ⅱ on collagen synthesis of HSCswas determined by 3H-proline incorporation.RESULTS Contrasted to the fibrosis in rats ofthe model group,groups of rats treated with either enalapril or Iosartan,or a combination oftwo drugs showed a limited expansion of theinterstitium(4.23±3.70 vs 11.22±4.79,P<0.05),but no difference was observedamong three treated groups(5.38±3.43,4.96±2.96,4.23±2.70,P>0.05).Expression of AT 1receptors was found in fibrotic interstitium offibrotic rats,whereas in normal control rats theywere limited to vasculature only to a very slightdegree.AT 1 receptors were also expressed onactivated HSCs in the culture.At concentrationsfrom 10-9to 10-5mol/L,Ang Ⅱ stimulated HSCproliferation in culture in a dose-dependentmanner.Increasing Ang Ⅱ concentrationsproduced corresponding increases in 3H-prolineincorporation.Differences among groups were significant.CONCLUSION Angiotensin-converting enzyme inhibitors and AT I blocker may slow the progression of hepatic fibrosis; activated HSCs express AT 1 receptors, and Ang Ⅱ can stimulate the proliferation and collagen synthesis of HSCs in a dose-dependent manner; and activation of RAS may be related to hepatic fibrogenesis induced by CCI4. | Hong Shan Wei Han Ming Lu Ding Guo Li Yu Tao Zhan Zhi Rong Wang Xin Huang Ji Lin Cheng Qin Fang Xu Department of Gastroenterology,Xinhua Hospital,Shanghai Second Medical University,Shanghai 200092,China | 2000 | World Journal of Gastroenterology2000，6，6： | 27 |
| 6 | Expression of bcl-2 protein in gastric carcinoma and its significance显示文摘Expressionofbcl2proteiningastriccarcinomaanditssignificanceLIUHaiFeng,LIUWeiWen,FANGDianChunandMENRongPuSubjectheadings... | LIU Hai Feng, LIU Wei Wen, FANG Dian Chun and MEN Rong Pu | 1998 | World Journal of Gastroenterology1998，4，3： | 23 |
| 7 | Charlson comorbidity index helps predict the risk of mortality for patients with type 2 diabetic nephropathy显示文摘研究目的:探讨Charlson合并症指数(CCI)对2型糖尿病肾病死亡率的预测作用。研究方法:建立2型糖尿病肾病研究队列,根据CCI评分将患者合并症的严重程度分为三度:轻度(CCI1–2分)、中度(CCI 3–4分)、重度(CCI≥5分)。将影响死亡率的因素及按CCI分层后的组间差异指标进行Logistic回归分析及方差分析(ANOVA)。Kaplan-Meier生存曲线分析CCI指数对生存时间及死亡率的影响。重要结论:533例2型糖尿病肾病患者纳入研究。所有患者的CCI评分均大于1,44.7%(238/533)的患者死亡。患者的死亡率随CCI评分增加而增加,CCI 1–2分患者的死亡率为21.0%(50/238),CCI 3–4分患者的死亡率为56.7%(135/238),CCI≥5分患者的死亡率为22.3%(53/238)。Logistic回归分析显示CCI评分、血红蛋白和血浆白蛋白水平是患者死亡率的预测因子(P<0.05)。方差分析结果显示,与CCI评分相对较低的患者相比,CCI评分越高的患者其血红蛋白水平较低,而血清肌酐较高,死亡率也更高。Kaplan-Meier生存曲线显示CCI评分越高的患者生存时间越短。CCI评分是一种简单易行且实用的评估疾病合并症的方法,可用于预测2型糖尿病肾病患者的死亡率。关注2型糖尿病肾病患者的合并症将有利于早期、有效治疗及改善预后。 | You-qun HUANG Rong GOU Yong-shu DIAO Qing-hua YIN Wen-xing FAN Ya-ping LIANG Yi CHEN Min WU Li ZANG Ling LI Jing ZANG Lu CHENG Ping FU Fang LIU | 2014 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2014，15，1： | 23 |
| 8 | Study on Relationship between Microstructure of Active Phase and HDS Performance of Sulfided Ni-Mo Catalysts: Effect of Metal Loading显示文摘Six Ni-Mo catalysts with different metal contents were prepared and characterized by N2 adsorption and X-ray diffractometry. The active phase microstructure of these catalysts was examined by the Raman spectroscopy, temperatureprogrammed reduction(TPR), X-ray photoelectron spectroscopy, and high-resolution transmission electron microscopy. Hydrodesulfurization(HDS) activity of catalyst samples were analyzed in a flow fixed-bed microreactor. The sulfidation degree of Mo and the length of the MoS2 slab slightly increased with the amount of metal loaded following sulfidation. This small change is attributed to polymolybdate species observed in all the oxidized catalysts. Weak metal-support interactions, as determined by the TPR technique, increased the NiSx sulfidation phase and MoS2 slab stacking. The HDS activity of the catalyst samples increased with the number of active sites. For high metal loading catalysts, their HDS activity was nearly identical because the sulfur atoms cannot easily approach active sites. This change is caused by the large number of stacked layers in the MoS2 slabs as well as the decrease in the specific surface area and pore volume of the catalyst samples with an increasing metal loading. | Guo Rong Shen Benxian Fang Xiangchen Sun Jin Peng Chong Cui Xiaoli | 2014 | China Petroleum Processing & Petrochemical Technology2014，16，2： | 21 |
| 9 | Application of nonlinear chemical fingerprinting to identification,evaluation and clinical use of Glycyrrhiza显示文摘Nonlinear chemical fingerprinting is a new technique for analyzing traditional Chinese medicines (TCMs). We developed such a method and applied it to Glycyrrhiza, a significant TCM. Nonlinear chemical fingerprints were obtained for Glycyrrhiza from four different production areas, and for 14 other TCMs with similar induction time. The calculation of their similarities was based on similarity system theory. Using intuitive visual comparison of the data and the similarity calculation results, Glycyrrhiza was discriminated from the 14 other TCMs. There was a linear relationship between the content of active TCM components and the induction time. This was used to compare the content of active components in Glycyrrhiza from the four different production areas, and establish an equivalent-dose relationship for these four samples. This method provides a new tool for discrimination and evaluation of Glycyrrhiza, and a dosage reference for clinical application of Glycyrrhiza from the four regions studied. | FANG XuanQi ZHANG TaiMing ZHAO Zhe XIANG FengQin LIANG YiZeng WANG Mei ZHU Rong CHEN Su QIAO JunXi | 2010 | Chinese Science Bulletin2010，55，26： | 20 |
| 10 | Hypothalamic-pituitary-adrenal axis hyperactivity accounts for anxiety-and depression-like behaviors in rats perinatally exposed to bisphenol A显示文摘Accumulating studies have proved that perinatal exposure to environmental dose causes long-term potentiation in anxiety/depression-related behaviors in rats.Hyperactivity of the hypothalamic-pituitary-adrenal(HPA) axis is one of the most consistent biological findings in anxiety- and depression-related disorders.The HPA axis is reported to be susceptible to developmental reprogramming.The present study focused on HPA reactivity in postnatal day(PND) 80 male rats exposed perinatally to environmental-dose BPA.When female breeders were orally administered 2 μg/(kg.day) BPA from gestation day 10 to lactation day 7,their offspring(PND 80 BPA-exposed rats)showed obvious anxiety/depression-like behaviors.Notably,significant increase in serum corticosterone and adrenocorticotropin,and corticotropin-releasing hormone mRNA were detected in BPA-exposed rats before or after the mild stressor.Additionally,the level of glucocorticoid receptor mRNA in the hippocampus,but not the hypothalamus,was decreased in BPA-exposed rats.The levels of hippocampal mineralocorticoid receptor mRNA,neuronal nitric oxide synthase and phosphorylated cAMP response element binding protein were increased in BPA-exposed rats.In addition,the testosterone level was in BPA-exposed rats.The results indicate that reprogramming-induced hyperactivity of the HPA axis is an important link between perinatal BPA exposure and persistent potentiation in anxiety and depression. | Fang Chen Libin Zhou Yinyang Bai Rong Zhou Ling Chen | 2015 | The Journal of Biomedical Research2015，29，3： | 17 |
| 11 | Determining method,conditional factors,traits and applications of nonlinear chemical fingerprint by using dissipative components in samples显示文摘The thermodynamic systems and dynamic model suitable for determining the nonlinear chemical fingerprints of samples were analyzed.The results indicated that the damp nonlinear chemical reactions in close systems away from the equilibrium and open systems without the complementarity of the dissipation substances have important significance for the throng characterization and whole content analysis of chemical components in samples.Various factors influencing on nonlinear chemical fingerprint,such as reactant species and their concentrations,electrode types,temperature,stir rate,the sort,dosage and granularity of the sample,etc.were amply researched by a nonlinear chemistry reaction,namely,damp B-Z oscillation which used acetone and glucose as the main dissipative substances.In addition,the quantitative information on the whole of chemical components in samples and the traits and applications of the fingerprint were investigated.The method and its important conditions for determining nonlinear chemistry fingerprint used in distinguishing and evaluating complex samples were successfully put forward. | ZHANG TaiMing ZHAO Zhe FANG XuanQi QIAO JunXi XIANG FengQin ZHU Rong LIANG YiZeng DING Feng | 2012 | Science China Chemistry2012，55，2： | 16 |
| 12 | Scalable preparation of porous silicon nanoparticles and their application for lithium-ion battery anodes显示文摘 | Mingyuan Ge Jiepeng Rong Xin Fang Anyi Zhang Yunhao Lu Chongwu Zhou | 2013 | Nano Research2013，6，3： | 16 |
| 13 | Prevalence, awareness, treatment, and control of hypertension in the non-dialysis chronic kidney disease patients显示文摘 | ZHENG Ying CAI Guang-yan CHEN Xiang-mei FU Ping CHEN Jiang-hua DING Xiao-qiang YU Xue-qing LIN Hong-li LIU Jian XIE Ru-juan WANG Li-ning NI Zhao-hui LIU Fu-you YIN Ai-ping XING Chang-ying WANG Li SHI Wei LIU Jian-she HE Ya-ni DING Guo-hua LI Wen-ge WU Guang-li MIAO Li-ning CHEN Nan SU Zhen MEI Chang-lin ZHAO Jiu-yang GU Yong BAI Yun-kai LUO Hui-min LIN Shan CHEN Meng-hua GONG Li YANG Yi-bin YANG Xiao-ping LI Ying WAN Jian-xin WANG Nian-song LI Hai-ying XI Chun-sheng HAO Li XU Yan FANG Jing-ai LIU Bi-cheng LI Rong-shan WANG Rong ZHANG Jing-hong WANG Jian-qin LOU Tan-qi SHAO Feng-min MEI Feng LIU Zhi-hong YUAN Wei-jie SUN Shi-ren ZHANG Ling ZHOU Chun-hua CHEN Qin-kai JIA Shun-lian GONG Zhi-feng GUAN Guang-ju XIA Tian ZHONG Liang-bao | 2013 | Chinese Medical Journal2013，，12： | 16 |
| 14 | Effect of anaphylatoxin C3a, C5a on the tubular epithelial-myofibroblast transdifferentiation in vitro显示文摘背景 Tubulointerstitial 肾的纤维变性是进步的肾疾病的普通结束点,并且管状的 epithelial-myofibroblast transdifferentiation ( TEMT )在 tubulointerstitial 的进步起一个关键作用肾的 fibrosis.Anaphylatoxin C3a 和 C5a 在肾的疾病并且作为这研究的潜在的新治疗学的 targets.The 目的作为新奇 profibrotic 因素被识别是调查是否 C3a , C5a 能由转变生长 factor-31 ( TGF-1 ) /connective 织物生长因素调整 TEMT 控制组, 10 ng/ml TGF-31 组, 50 nmol/L C5a 组,加 2.5 mol/L C5aRA 的 50 nmol/L C5a group.TGF-1 受体对手( TGF-1 RA ) 10 g/ml 被用来调查 导致C3a 、 导致C5a 的 TEMT.Electron 的机制显微镜学被用来观察染色的词法 changes.Immunocytochemistry ,即时 PCR 并且西方的弄污被用来检测光滑的肌肉肌动朊的表情( -SMA),E-cadherin,Col-I,C3a 受体( C3aR ), C5aR , CTGF 和与 C3a 和 C5a fo 有教养的 TGF-1.Results HK-2 | LIU Fang GOU Rong HUANG Jun FU Ping CHEN Feng FAN Wen-xing HUANG You-qun ZANG Li WU Min QIU Hong-yu WEI Da-peng | 2011 | Chinese Medical Journal2011，，23： | 15 |
| 15 | Principle of nonlinear chemical fingerprint by using dissipative components in samples as well as calculation and evaluation of similarity显示文摘Under the conditions of constant temperature and pressure,different influences of samples with different chemical components on the mechanism of nonlinear chemical reaction will cause different changes of the potential-time relationship curve of the nonlinear chemical reaction system.Using it as the character,and using the B-Z nonlinear chemical system to use acetone and substrates in samples as main dissipative substances qua an example,the principle of nonlinear chemical fingerprint has been researched and discussed in detail.At the same time,the general method for calculating the system similarity about nonlinear chemical fingerprint was also put forward,and similarities of nonlinear chemistry fingerprints of different batches of Guhan Yangshengjing and 18 sorts of other samples were calculated by Euclidean distance,correlation coefficient,included angle cosine and system similarity,at the same time,the various similarities were analyzed.The results showed that,both of correlation coefficient and included angle cosine are unable to be used as the criterion for quantitatively evaluating the similarity of nonlinear chemistry fingerprint;as non-parametric similarity,Euclidean distance can accurately reflect the feature differences in the fingerprints,but as parametric similarity,sometimes,Euclidean distance can not accurately reflect the relative extent of characteristic difference in the nonlinear chemical fingerprints;system similarity can most truthfully reflect the characteristic difference in the nonlinear chemical fingerprints,and is the best evaluating method among the four ones.Therefore,system similarity can be used to quantitatively calculate the similar extent between the nonlinear chemical fingerprints.An economical,simple and convenient,easy pushing and effective method for identifying and evaluating complicated samples has successfully been put forward. | ZHANG TaiMing ZHAO Zhe FANG XuanQi QIAO JunXi XIANG FengQin ZHU Rong LIANG YiZeng DING Feng | 2012 | Science China Chemistry2012，55，2： | 13 |
| 16 | Expressions of chromogranin A and cathepsin D in human primary hepatocellular carcinoma显示文摘AIM To determine the expression and clinicalsignificance of chromogranin A and cathepsin Din hepatocellular carcinoma(HCC).METHODS Double immunofluorescence stain-ing techniques combined with laser confocalscanning microscopy(LSCM)was used toinvestigate chromogranin A and cathepsin Dexpressions in 85 HCC patients.RESULTS Cathepsin D was expressed in :3normal liver tissues,while in HCC the stainingshowed regional variation and the fraction ofstrongly stained cells increased as the tumorsbecame less differentiated and usually clinicallymore malignant.Cells which showed strongpositivity for cathepsin D were present in 71/85(83.5%)cases.Strong expression of cathepsinD in cancer cells was related tohistopathological features.They were morecommon in grade 3-4(26/28,92.9%)and grade2(46/53,86.8%)tumors than in grade 1 tumors(1/4,25.0%)(P<0.01).No significantcorrelation was found between age andcathepsin D expression.In patients with positivecathepsin D reaction,the mean age was 52.1±2.8 years(range 32-68 years)and in the groupwith negative reaction,the mean age was 51.3±4.5 years(range 28-71 years).No obvious relationship was observed between CgAexpression in cancer cells and thehistopathological features.The CgA positiverate was 75.0%(3/4)in grade 1,71.7%(38/53)in grade 2,and 71.4%(20/28)in grade 3-4(P>0.05)tumors.The coexpression of CgA andcathepsin D was found by double labeledimmunofluorescence staining techniques.Theprocessing of cathepsin D was disturbed in HCCcells and accumulated in the cells.Cathepsin Dhad proteolytic activity and autocrine mitogeniceffect,suggesting their functions in invasion.These findings demonstrated that the expressionof cathepsin D in HCC had prognostic value.CONCLUSION Chromogranin A and cathepsin Dare expressed in a high proportion of HCC andthe existence of cathepsin D in HCC might berelated to processing of CgA.This is clearly asubject for further studies because of itspotential clinical applications. | Xiao Feng Huang Chun Mei Wang Xiao Wen Dai Zhen Jiang Li Bo Rong Pan Li Bin Yu Bin Qian Li Fang | 2000 | World Journal of Gastroenterology2000，6，5： | 12 |
| 17 | Expression changes of activin A in the development of hepatic fibrosis显示文摘AIM To examine the expression of activin A, amember of the transforming growth factor (TGF-β) superfamily, recently has been reported to beoverexpressed in liver cirrhosis, in the course ofcarbon tetrachloride-induced rat hepaticfibrosis.METHODS Hepatic fibrosis was induced in ratsby subcutaneous injections of 40% carbontetrachloride oily solution for a period of 1 to 7weeks. At the end of 1, 2, 3, 4, 5, 6 and 7 weeksafter carbon tetrachloride injections, the ratswere killed in group (6- 10 rats each time) forstudy. The activin A messenger RNA expressionand its protein localization were assessed bysemi-quantitative reverse transcriptionpolymerase chain reaction ( RT-PCR ) andimmunohistochemistry.RESULTS The normal rat liver expressedactivin A mRNA and protein, and its expressionwas transiently decreased and becameundetectable after carbon tetrachlorideinjections for 2 or 3 weeks and then increasedgradually. After injection of carbon tetrachloridefor 6 and 7 weeks, activin A mRNA and proteinexpressions were significantly enchanced in ratliver. Compared with that of the normal ratliver. Activin A mRNA expression levels in ratsreceiving carbon tetrachloride injections for 6and 7 weeks were 1.6 and 2.2 times that of thosein normal rat liver respectively (0.456±0.094 vs0.286± 0.0670, P<0.01; 0.620± 0.134 vs 0.286±± 0.0670, P<0.01). Immunohistochemistryshowed that activin A expressed in hepatocytesof normal liver, and its expression wasdecreased in rats receiving carbon tetrachloridefor 2 or 3 weeks. Compared with normal liver,activin A expression distribution mode changedin fibrotic liver, being increased significantly inhepatocytes around fibrotic areas.CONCLUSION Activin A expression wasincreased in latestage of hepatic fibrosis, andthis may be involved in hepatic fibrosisformation in this period. | Xin Huang1 Ding Guo Li1 Zhi Rong Wang1 Hong Shan Wei1 Ji Lin Cheng1 Yu Tao Zhan1 Xin Zhou1 Qin Fang Xu1 Xin Li2 Han Ming Lu1 1Xinhua Hospital, Shanghai Second Medical University, Shanghai 200092, China2Ninth Hospital. Shanghai Second Medical University, Shanghai 200011. China | 2001 | World Journal of Gastroenterology2001，7，1： | 12 |
| 18 | Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbfα-1 pathway显示文摘In this paper,we investigate the effect and the possible mechanism of high glucose levels on the calcification of human aortic smooth muscle cells (HASMCs).HASMCs were divided into four groups: normal glucose group (NG),osmolality control group (OC),high glucose group (HG,HASMCs culture medium containing 30 mmol/L glucose),and high glucose plus recombinant human Noggin protein (bone morphogenetic protein-2 (BMP-2) antagonist) group (HN).The mRNA levels and the protein expressions of BMP-2 and core binding factor alpha-1 (Cbfα-1) were measured by real-time quantitative polymerase chain reaction (PCR) and Western blot.After induced by 10 mmol/L β-glycerol phosphoric acid,cells were harvested for assessments of alkaline phosphatase (ALP) activities at Days 1,2,and 3,and intracellular calcium contents at Days 7 and 14,respectively.High glucose levels increased the mRNA levels and the protein expressions of BMP-2 and Cbfα-1 (P<0.05).The expression of Cbfα-1 was partially blocked by Noggin protein (P<0.05),while BMP-2 was not (P>0.05).After being induced by β-glycerol phosphoric acid,high glucose levels increased the ALP activity [(48.63±1.03) vs.(41.42±2.28) U/mg protein,Day 3;P<0.05] and the intracellular calcium content [(2.76±0.09) vs.(1.75±0.07) μmol/mg protein,Day 14;P<0.05] in a time-dependent manner when compared with the NG group,while the ALP activity could not be blocked by Noggin protein [(48.63±1.03) vs.(47.37±0.97) U/mg protein,Day 3;P>0.05].These results show that high glucose levels can evoke the calcification of HASMCs by inducing osteoblastic trans-differentiation and intracellular calcium deposition via the BMP-2/Cbfα-1 pathway,which can be partially blocked by Noggin protein. | Fang LIU Hui ZHONG Jing-yuan LIANG Ping FU Zhi-juan LUO Li ZHOU Rong GOU Jun HUANG | 2010 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2010，11，12： | 12 |
| 19 | Mammalian target of rapamycin pathway inhibition enhances the effects of 5-aza-dC on suppressing cell proliferation in human gastric cancer cell lines显示文摘The present study aimed to evaluate the relationship between mTOR signaling pathway and DNA methylation in cell survival,cell cycle,gene expression and protein level on human gastric cancer cells. Human gastric cancer cell lines,MKN45 and SGC7901 were treated with 5-aza-dC,rapamycin and/or LY294002.Cell viability was analyzed by MTT.Cell cycle distribution was evaluated by flow cytometry (FCM).The transcription level of PTEN and p27 Kip1 genes was detected by using real-time PCR.Protein expressions were detected by Western blotting.We found that cell viability was moderately reduced when treated with 5-aza-dC alone,but remarkably reduced when mTOR pathway was inhibited together (P<0.01).mTOR inhibition enhances the effects of 5-aza-dC on arresting cell cycle at G2 phase in human gastric cancer cell lines.The expression of PTEN and p27 Kip1 mRNA was remarkably increased in the gastric cancer cells treated with combind drugs(P<0.01).Phosphorylation of Akt,p70S6K and 4E-BP1 were significantly reduced in the cells treated with LY294002 or RAPA(P<0.01),but we failed to find that 5-aza-dC enhance these effects.We suggested that mTOR inhibition could enhance the effects of 5-aza-dC on suppressing cell proliferation and arresting cell cycle in human gastric cancer cell lines, which might be a potential target for tumor therapy. | SUN DanFeng,TIAN XiaoQing,ZHANG YanJie,CHEN YingXuan,LU Rong,WANG Xia &FANG JingYuan Shanghai Jiao-Tong University Sc0hool of Medicine Renji Hospital,Shanghai Institute of Digestive Disease,Shanghai 200001, China | 2008 | Science China(Life Sciences)2008，51，7： | 12 |
| 20 | Performance characterization of Ni60-WC coating on steel processed with supersonic laser deposition显示文摘Ni60-WC particles are used to improve the wear resistance of hard-facing steel due to their high hardness. An emerging technology that combines laser with cold spraying to deposit the hard-facing coatings is known as supersonic laser deposition. In this study, Ni60-WC is deposited on low-carbon steel using SLD. The microstructure and performance of the coatings are investigated through SEM, optical microscopy, EDS, XRD, microhardness and pin-on-disc wear tests. The experimental results of the coating processed with the optimal parameters are compared to those of the coating deposited using laser cladding. | Fang LUO Andrew COCKBURN Martin SPARKES Rocco LUPOI Zhi-jun CHEN William O'NEILL Jian-hua YAO Rong LIU | 2015 | Defence Technology(防务技术)2015，11，1： | 11 |
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