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| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | High efficiency, high power 808nm laser array and stacked arrays optimized for elevated temperature operation显示文摘Operation of 808-nm laser diode pumping at elevated temperature is crucial to many applications. Reliable operation at high power is limited by high thermal load and low catastrophic optical mirror damage (COMD) threshold at elevated temperature range. We demonstrated high efficiency and high power operation at elevated temperature with high COMD power. These results were achieved through device design optimization such as growth conditions, doping profile, and materials composition of the quantum-well and other layers. Electrical-to-optical efficiency as high as 62% was obtained through lowered threshold current, lowered series resistance and increased slope efficiency. The performance of single broad-area laser diodes scales to that of high power single bars on water-cooled copper micro-channel heatsinks or conductively-cooled CS heatsinks. No reduction in bar performance or significant spectral broadening is seen when these micro-channel coolers are assembled into 6-bar and 18-bar CW stacks for the highest power levels. | Crump P A Crum T R DeVito M Farmer J Grimshaw M Huang Z Igl S A Macomber S Thiagarajan P Wise D | 2004 | 光机电信息2004，21，6： | 4 |
| 3 | High performance n-type carbon nanotube field-effect transistors with chemically doped contacts 显示文摘 | JAVEY A TU R FARMER DB | 2005 | Nano Lett2005，5，2： | 1 |
| 4 | Isolation and properties of teleostean growth hormone显示文摘 | Farmer S W H Papoff T A Bensley | 1976 | Gen Comp Endocrinol1976，30，： | 1 |
| 5 | Regulation of expression of proteinase inhibitor genes by methyl jasmonate and jasmonic acid 显示文摘 | FARMER E E JOHNSON R R RYAN C A | 1992 | Plant Physio11992，98，： | 1 |
| 6 | Morphometric relationships of commercially important species of penaeid shrimp from the Arabian Gulf显示文摘 | FARMER A S D | 1986 | Kuwait Bull Mar Sci1986，7，： | 1 |
| 7 | Type 2 diabetes and cardiovascular risk factors显示文摘 | Sharma MD Farmer JA Garber A | 2011 | Current Med Res Opinion2011，27，3： | 1 |
| 8 | Prevalence of chronic disabling fatigue in children and adolescents 显示文摘 | Farmer A Fowler T Scourfield J | 2004 | BrJ Psychiatry2004，，184： | 1 |
| 9 | The origin of lead in the organic horizon of tundra soils: atmospheric deposition,plant translocation from the mineral soil or soil mineral mixing 显示文摘 | KIAMINDER J FARMER J G MACKENZIE A B | 2011 | Science of the Total Environment2011，409，20： | 1 |
| 10 | Regulating of expression of proteinase inhibitor genes by methyl jasmonate and jasmonic acid 显示文摘 | Farmer E E Johnson R R Ryan C A | 1992 | Plant Physiol1992，98，： | 1 |
| 11 | High performance n type carbon nanotube field effect transistors with chemically doped contacts显示文摘 | JAVEY A TU R FARMER DB | 2005 | Nano Letters2005，5，2： | 1 |
| 12 | Oligosaccharide signals in plants: a current assessment显示文摘 | RYAN C A FARMER E E | 1991 | Annual Review of Plant Physiology and Plant Molecular Biology1991，42，： | 1 |
| 13 | A bilateral gynandromorphy of Nephrops norvegicus (Decapoda, Nephropidae)显示文摘 | Farmer A S | 1972 | Mar Biol1972，15，： | 1 |
| 14 | Timing of volcanism in the Sierra Nevada of California:Evidence for Pliocene delamination of the batholithic root?显示文摘 | Manley C R Glazner A F Farmer G L | 2000 | Geology2000，28，： | 1 |
| 15 | Oligosaccharide signals in plants: A current assessment 显示文摘 | Ryan C A Farmer E F | 1991 | Annu Rev Phy-Siol Mol Biol1991，42，651： | 1 |
| 16 | Drug -induced liver injury显示文摘 | FARMER AD BRIND A | 2011 | Medicine2011，39，9： | 1 |
| 17 | Neuroticism, extraver sion,life events and depression: the cardiff depression study 显示文摘 | Farmer A Redman K Harlis T | 2002 | Br J Psychiatry2002，181，： | 1 |
| 18 | Enterobacter Skazakii:A New Species of 'Enterbacterlaceae'lsolated from Clinical Deci rrlerls显示文摘 | FARMER J J ASBURY M A HICKMAN F W | 1980 | Int H Syst Bacterlol1980，30，： | 1 |
| 19 | The efficacy of drugs for the treatment of LUTS/BPH,a study in 6 European countries显示文摘 | Hutchison A Farmer R Verhamme K | 2007 | Eur Uro12007，51，1： | 1 |
| 20 | Did lithospheric delamination trigger late Cenozoic potassic volcanism in the southern Sierra Nevada,California?显示文摘 | Farmer G L Glazner A F Manley C R | 2002 | Geological Society of America Bulletin2002，114，： | 1 |
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