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| 1 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 2 | 基于定量宏基因组学评估肝硬化患者肠道菌群的变化与慢加急性肝衰竭及预后的关系显示文摘【据Gastroenterology 2020年9月报道】题:基于定量宏基因组学评估肝硬化患者肠道菌群的变化与慢加急性肝衰竭及预后的关系(作者SoléC等)肝硬化与肠道菌群组成的改变有关。慢加急性肝衰竭(ACLF)是肝硬化最严重的临床阶段,但目前仍缺乏使用定量宏基因组学研究ACLF中肠道菌群改变的信息。 | SOLéC GUILLY S Da SILVA K 邵玥明 温晓玉 | 2020 | 临床肝胆病杂志2020，36，11： | 4 |
| 3 | Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in rats显示文摘AIM To evaluate the sex-specific effects of a hydroalcoholic extract from Eugenia punicifolia(HEEP) leaves on gastric ulcer healing.METHODS In this rat study involving males, intact(cycling) females, and ovariectomized females, gastric ulcers were induced using acetic acid. A vehicle, lansoprazole, or HEEP was administered for 14 d after ulcer induction. Body weight was monitored throughout the treatment period. At the end of treatment, the rats were euthanized and the following in vivo and in vitro investigations were performed: macroscopic examination of the lesion area and organ weights, biochemical analysis, zymography, and evaluation of protein expression levels. Additionally, the concentration-dependent effect of HEEP was evaluated in terms of subacute toxicity and cytotoxicity.RESULTS Compared to the vehicle, HEEP demonstrated a great healing capacity by substantially reducing the ulcerative lesion area in males(52.44%), intact females(85.22%), and ovariectomized females(65.47%), confirming that HEEP accelerates the healing of acetic acidinduced gastric lesions and suggesting that this effect is modulated by female sex hormones. The antiulcer effect of HEEP was mediated by prostaglandin E2 only in male rats. Overall, the beneficial effect of HEEP was the highest in intact females. Notably, HEEP promoted the expression of vascular endothelial growth factor(intact vs ovariectomized females) and decreased the expression of Caspase-8 and Bcl-2(intact female vs male or ovariectomized female). Additionally, HEEP enhanced fibroblast proliferation and migration into a wounded area in vitro, confirming its healing effect. Finally, no sign of subacute toxicity or cytotoxicity of HEEP was observed.CONCLUSION In gastric ulcers, HEEP-induced healing(modulated by female sex hormones; in males, mediated by prostaglandin) involves extracellular matrix remodeling, with gastric mucosa cell proliferation and migration. | Larissa Lucena Périco Vinícius Peixoto Rodrigues Rie Ohara Gabriela Bueno Vania Vasti Alfieri Nunes Raquel Cássia dos Santos Ana Carolina Lima Camargo Luis Antuio Justulin Joior Sérgio Faloni de Andrade Viviane Miranda Bispo Steimbach Luísa Mota da Silva Lúcia Regina Machado da Rocha Wagner Vilegas Catarina dos Santos Clélia Akiko Hiruma-Lima | 2018 | World Journal of Gastroenterology2018，24，38： | 3 |
| 4 | Acute fatty liver of pregnancy associated with severe acute pancreatitis:A case report显示文摘Acute fatty liver of pregnancy is a rare disease that affects women in the third trimester of pregnancy. Although infrequent, the disease can cause maternal mortality. The diagnosis is not always clear until the pregnancy is terminated, and significant complications, such as acute pancreatitis, can occur. Pancreatic involvement typically only occurs in severe cases after the development of hepatic and renal impairment. To date, little knowledge is available regarding how the disease causes pancreatitis. Treatment involves supportive measures and pregnancy interruption. In this report, we describe a case of a previously healthy 26-year-old woman at a gestational age of 27 wk and 6 d who was admitted with severe abdominal pain and vomiting. This case illustrates the clinical and laboratory overlap between acute fatty liver of pregnancy and pancreatitis, highlighting the difficulties in differentiating each disease. Furthermore, the hypothesis for this overlapping is presented, and the therapeutic options are discussed. | Cássio Vieira de Oliveira Alecsro Moreira Julio P Baima Leticia de C Franzoni Talles B Lima Fabio da S Yamashiro Kunie Yabuki Rabelo Coelho Ligia Y Sassaki Carlos Antonio Caramori Ferno G Romeiro Giovanni F Silva | 2014 | World Journal of Hepatology2014，6，7： | 3 |
| 5 | Transmission capacity: availability, maximum, transfer and reliability显示文摘 | Leite da S A M de Carvalho C J G da Fonseca M L A | 2002 | IEEE Transactions on Power Systems2002，17，3： | 1 |
| 6 | Effect of ausforming on microstructure and hardness of AISI H13 tool steel modified with niobium显示文摘 | Elias C N and Costa Viana C S Da | 1992 | Materials Science and Technology1992，9，： | 1 |
| 7 | Application of a novel fuzzy classifier to fault detection and isolation of the DAMADICS bench_mark problem显示文摘 | BOCANIALA C D DA COSTA J S | 2006 | Control Engineering Practice2006，14，6： | 1 |
| 8 | Chemical composition and free radical scavenging activity of pollen loadsfrom stingless bee Melipona subnitida D\icke显示文摘 | Silva T M S Camara C A da Silva Lins A C | 2006 | Journal of Food Composition and Analysis2006，19，6： | 1 |
| 9 | Prediction of core saturation instability at an HVDC converter显示文摘 | Burton R S Fuchshuber C Woodford DA | 1996 | IEEE Trans on Power Delivery1996，11，4： | 1 |
| 10 | Probiotic potential of Minas fresh cheese prepared with the addition of Lactobacillus paracasei 显示文摘 | BURITI F C A DA ROCHA J S ASSIS E G | 2005 | Lebensmittel-Wissenschaft und Tehonlogie2005，38，2： | 1 |
| 11 | Experimental assessment of perfobond and T- perfobond shear connectors' structural response 显示文摘 | Vianna J da C Costa-Neves L F da S Vellasco P C G | 2008 | Journal of Constructional Steel Research2008，65，2： | 1 |
| 12 | Inhibitory action of aqueous coffee ground extracts on the corrosion of carbon steel in HC1 solution 显示文摘 | TORRESS V V AMADO R S de sAC F FERNANDEZ T L da SILVA RIEHI C A TORRES A G D'ELIA E | 2011 | Corrosion Science2011，53，： | 1 |
| 13 | A parametric study of forced convection in an enclosure with stationary heated cylinders显示文摘 | Guimar?es P M Da Silva C E S | 2010 | International Communications in Heat and Mass Transfer2010，37，5： | 1 |
| 14 | Online voltage stability monitoring显示文摘 | DE SOU ZA A C Z DE SOUZA J C S DA SILVA A M L | 2000 | IEEE Trans on Power Systems2000，15，4： | 1 |
| 15 | Study of the preparation and catalytic performance of molybdenum carbide catalysts prepared with C2H2/H2 carburizing mixture显示文摘 | XIAO T C WANG H T DA J W COLEMAN K S GREEN M L | 2002 | J Catal2002，211，1： | 1 |
| 16 | Structural behaviour of T-perfobond shear connectors in composite girders: An experimental approach显示文摘 | Vianna J da C Costa-Neves L F da S Vellasco P C G | 2008 | Engineering Structures2008，30，9： | 1 |
| 17 | Retival VEGF mRNA measured by SYBR green Ⅰ fluorescence: A versatile approach to quantitative PCR显示文摘 | Simpson DA Feeney S Boyle C Stitt AW | 2000 | Mol Vis2000，6，10： | 1 |
| 18 | Detailed aH and 13C NMR structural assignment of three biologically active lignan lactones 显示文摘 | Vladimir C G H Rosangela da S Susimaire P | 2006 | Spectroehim Aeta PartA2006，63，： | 1 |
| 19 | Bad design, bad practices, bad bugs: frustrations in controlling an outbreak of Elizabe- thkingia meningoseptica in intensive care units 显示文摘 | Balm MN Salmon S Jureen R Teo C Mahdi R Seetoh T Teo JT Lin RT Fisher DA | 2013 | J Hosp Infect2013，85，2： | 1 |
| 20 | J Org Chem显示文摘 | LiuY Da C S Yu S L | 2010 | 75: 6869-68782010，75，： | 1 |
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