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| 1 | Osteoprotegerin: A Novel Secreted Protein Involved in the Regulation of Bone Density显示文摘 | W.S Simonet D.L Lacey C.R Dunstan M Kelley M.-S Chang R Lüthy H.Q Nguyen S Wooden L Bennett T Boone G Shimamoto M DeRose R Elliott A Colombero H.-L Tan G Trail J Sullivan E Davy N Bucay L Renshaw-Gegg T.M Hughes D Hill W Pattison P Campbell S Sander G Van | 1997 | Cell1997，，2： | 6 |
| 2 | In vivo anti-tumor activity of murine hematopoietic stem cells expressing a p185HER2-specific chimeric T-cell receptor gene显示文摘We have confirmed efficient anti-tumor activities of the peripheral lymphocytes transduced with a p185HER2-specific chimeric T-cell receptor gene both in murine and in human in our previous studies. To further test the feasibility of chimeric T-cell receptor in a bone marrow transplantation model, we first, made two murine tumor cell lines: MT901 and MCA-205, to express human p185HER2 by retroviral gene transduction. Murine bone marrow cells were retrovirally transduced to express the chimeric T-cell receptor and gene-modified bone marrow cells were transplanted into lethally irradiated mouse. Six months post transplantation, p185HER2-positive tumor cells:MT-901/HER2 or MCA-205/ HER2 was subcutaneously or intravenously injected to make mouse models simulating primary breast cancer or pulmonary metastasis. The in vivo anti-tumor effects were monitored by the size of the subcutaneous tumor or counting the tumor nodules in the lungs after India ink staining. The size of the subcutaneous tumor was significantly inhibited and the number of pulmonary nodules were significantly decreased in mouse recipients transplanted with chimeric T-cell receptor modified bone marrow cells compared with the control group. Our results suggest the efficient in vivo anti-tumor activities of chimeric T-cell receptor gene modified bone marrow cells. | JIAN MIN YANG MICHAEL S FRIEDMAN MARIANNE T HUBEN JENNIFER FULLER QIAO LI ALFRED E CHANG JAMES J MULE KEVIN T MCDONAGH | 2006 | Journal of Microbiology and Immunology2006，4，2： | 3 |
| 3 | 转染嵌合性T细胞受体基因小鼠T淋巴细胞的体外抗肿瘤作用显示文摘目的:研究小鼠T淋巴细胞在转染嵌合性T细胞受体基因后的体外抗肿瘤作用。方法:应用重组DNA技术,将HER2特异性嵌合性T-细胞受体构建入逆转录病毒载体;转入包装细胞后,收集病毒上清,转染小鼠T淋巴细胞,转基因后的小鼠T淋巴细胞分别与HER2阳性(SK-OV-3)或阴性(MCF-7)的肿瘤细胞系共培养,检测其细胞因子γ干扰素释放,51Cr释放法检测CTL评价其抗肿瘤效应。结果:所构建载体经酶切鉴定符合要求,乒乓法转染包装细胞系GP+E86,检测病毒滴度为1.2×106,Retronectin结合离心法转染经抗CD3/CD28单抗活化的小鼠T淋巴细胞,转染效率可达50%以上;转染嵌合性T细胞受体基因的T淋巴细胞与HER2阳性或阴性的肿瘤细胞系共培养后可检测到HER2特异性的细胞因子γ干扰素释放,51Cr释放法测CTL可见转染嵌合性T细胞受体基因T淋巴细胞对HER2阳性的肿瘤细胞具显著杀伤效应。结论:转染嵌合性T细胞受体基因的小鼠T淋巴细胞在体外可通过细胞因子释放和CTL效应发挥显著的抗肿瘤作用。 | 杨建民 Michael S Friedman 李峤 James J Mule Alfred E Chang Kevin T McDonagh | 2006 | 中国肿瘤生物治疗杂志2006，13，4： | 2 |
| 4 | Prestress Influence on Shear Lag Effect in Continuous Box Girder Bridge显示文摘 | Chang S T | 1992 | J Struct Engrg ASCE1992，118，11： | 2 |
| 5 | Adaptive guaranteed cost control of systems with uncertain parameters显示文摘 | Chang M S L Peng T K C | 1972 | IEEE Transactions on Automatic Control1972，17，4： | 2 |
| 6 | Tracking design of uncertain nonlinear SISO systems:adaptive fuzzy approach显示文摘 | CHEN B S LEE T S CHANG Y C | 1994 | IEEE Transactions on Fuzzy Systems1994，4，2： | 2 |
| 7 | Adsorption and diffusion of aromatics in AIPO4-5显示文摘 | CHIANG A S T LEE C K CHANG Z H | 1991 | Zeolites1991，11，4： | 1 |
| 8 | Effect of selective thermal denaturation of seybean proteins on soymilk viscosity and tofu's physical properties显示文摘 | Liu Z S Chang S K C Li L T Tatsumi E | 2004 | Food Research International2004，37，： | 1 |
| 9 | Fundamental factors afecting biomass enzymatic reactivity 显示文摘 | CHANG V S HOLTZAPPLE M T | 2000 | Appl Biochem Biotech · nol2000，，848586： | 1 |
| 10 | Behavior of double skin composite wall subjected to in- plane cyclic loading显示文摘 | Eom T S Park H G Lee C H Kim J H Chang I H | 2009 | Journal of Structural Engineering ASCE2009，135，10： | 1 |
| 11 | Association of polymorphisms of interleukin l beta gene and Helicobacter pylori infection with the risk of gastric ulcer 显示文摘 | CHANG Y T WU M S SHUN C T | 2002 | J Gastrocnterol Hepatol2002，49，47： | 1 |
| 12 | Biodegradation of phenanthrene and pyrene fi-om mangrove sediment in subtropical Taiwan显示文摘 | CHANG B V CHANG I T YUAN S Y | 2008 | J environ sci health2008，43，3： | 1 |
| 13 | Independent factors associated with recurrent bleeding in cirrhotic patients with esophageal variceal hemorrhage显示文摘 | Lee S W Lee T Y Chang C S | 2009 | Dig Dis Sci2009，54，5： | 1 |
| 14 | Chemical composition and antifungal activity of essential oils from different tissues of Japanese Cedar (Cryptomeria japonica)显示文摘 | Cheng S S Lin H Y Chang S T | 2005 | J Agrie Food Chem2005，53，3： | 1 |
| 15 | Flow behavior of high strength high-performance concrete 显示文摘 | YEN T TANG C W CHANG C S CHEN K H | 1999 | Cement and Concrete Composites1999，21，： | 1 |
| 16 | H∞ tracking design of uncertain nonlinear SISO systems, Adaptive fuzzy approach显示文摘 | Chen B S Lee T S Chang Y C | 1999 | IEEE Trans on Fuzzy Systems1999，4，1： | 1 |
| 17 | Optimal pricing and ordering policy under permissible delay in payments显示文摘 | Teng J T Chang C T Goyal S K | 2005 | International Journal of Production Economics2005，97，： | 1 |
| 18 | Real-time garbage collection for flash-memory storage systems of realtime embedded systems 显示文摘 | Chang L P Kuo T W Lo S W | 2004 | ACM Transactions on Embedded Computing Systems2004，3，4： | 1 |
| 19 | Interface dynamics and mechanisms of nanoindented alkanethiol self-assembled monolayers using molecular simulations 显示文摘 | Fang T H Chang W Y Lin S J | 2010 | Journal of Colloid and Interface Science2010，345，： | 1 |
| 20 | Three-axis machining of compound surfaces using flat and filleted end mills显示文摘 | HWANG J S CHANG T C | 1998 | CAD1998，30，8： | 1 |
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