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| 1 | Glutathione Peroxidase Revisited—Simulation of the Catalytic Cycle by Computer-Assisted Molecular Modelling显示文摘Glutathione peroxidase, the first example of selenoproteins identified in mammals, was subjected to force field calculations and molecular dynamics in order to enable a clearer comprehension of enzymatic selenium catalysis. Starting from the established X-ray structure of bovine GPX, all kinetically defined intermediates and enzyme substrate complexes were modelled. The models thus obtained support the hypothesis that the essential steps of the catalysis are three distinct redox changes of the active site selenium which, in the ground state, presents itself at the surface of selenoperoxidases as the center of a characteristic triad built by selenocysteine, glutarnine and tryptophan. In GPX, four arginine residues and a lysine residue provide an electrostatic architecture which, in each reductive step, directs the donor substrate GSH towards the catalytic center in such a way that 1ts sulfhydryl group must react with the selenium moiety. To this end, different equally efficient modes of substrate binding appear possible. The models are consistent with substrate specificity data, kinetic pattern and other functional characteristics of the enzyme. Comparison of molecular models of GPX with those of other members of the GPX superfamily reveals that the cosubstrate binding mechanisrns are unique for the classical type of cytosolic glutathione peroxidases but cannot operate e. g. in plasma GPX and phospholipid hydroperoxide GPX. The structural differences between the selenoperoxidases, shown to be relevant to their specificities, are discussed in terms of functional diversification within the GPX | K. -D. AUMANN N. BEDORF R. BRIGELIUS-FLOHED D. SCHOMBURG AND L. FLOHE(Gesellschaft fur Biotechnologische Forschung mbH (GBF) Mascheroder Weg 1, D-38124 Braunschweig, Germany Deutsches Institut fur Ernahrungsforschung (DIfE) Arthur-Scheunert-Allee 114 | 1997 | Biomedical and Environmental Sciences1997，10，2： | 6 |
| 2 | NONLINEAR INVERSE SIMULATION FOR THE MANEUVERING FLIGHT OF COAXIAL ROTOR HELICOPTERS显示文摘NONLINEARINVERSESIMULATIONFORTHEMANEUVERINGFLIGHTOFCOAXIALROTORHELICOPTERSCaoYihua(InstituteofAircraftDesign,BeijingUniversit... | Cao Yihua (Institute of Aircraft Design, Beijing University of Aeronautics and Astronautics, Beijing, 100083, China) G. Reichert (Technical University of Braunschweig, 38106 Braunschweig, Germany) | 1997 | Chinese Journal of Aeronautics1997，10，4： | 3 |
| 3 | Enteral compared with parenteral nutrition: a mete-analysis显示文摘 | Braunschweig CL Levy P Sheean PM | 2001 | Am J Clin Nutr2001，74，4： | 1 |
| 4 | Enteral compared with parenteral nutrition:A meta-analysis显示文摘 | Braunschweig CL Levy P Sheean PM | 2001 | Am J Clin Nutr2001，74，： | 1 |
| 5 | T- and B-cell responses to different hepatitis C virus antigens in patients with chronic hepatitis C infection and in healthy anti-hepatitis C virus-positive blood donors without viremia显示文摘 | Lechmann M lhlenfeldt HG Braunschweiger I | 1996 | Hepatology1996，24，4： | 1 |
| 6 | A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig 显示文摘 | Van Laere A S Nguyen M Braunschweig M | 2003 | Nature2003，425，6960： | 1 |
| 7 | Evaluation of spiral CT in staging of colon and rectum carcinoma显示文摘 | Hundt W Braunschweig R Reiser M | 1999 | Eur Radiol1999，9，： | 1 |
| 8 | Intrathoracic impedance monitoring, audible patient alerts, and outcome in patients with heart failure 显示文摘 | van Veldhuisen D J Braunschweig F Conraads V | 2011 | Circulation2011，124，16： | 1 |
| 9 | Carotid plaque analysis: comparison of dual-source computed tomography ( CT ) findings and histopathological correlation显示文摘 | Das M Braunschweig T Muhlenbruch G | 2009 | Eur J Vasc Endovasc Surg2009，38，1： | 1 |
| 10 | Impact of declines in nutritional status on outcomes in adult patients hospitalized for more than 7 days 显示文摘 | Braunschweig C Gomez S Sheean PM | 2000 | J Am Dietetic Assoc2000，100，11： | 1 |
| 11 | Pitfalls in TRAP assay in routine detection of malignancy in effusions显示文摘 | Braunschweig R Guilleret I Delacretaz F | | 0，，04： | 1 |
| 12 | A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig显示文摘 | Van Laere AS Nguyen M Braunschweig M | 2003 | Nature2003，425，6960： | 1 |
| 13 | Can monitoring of intrathoracic impedance reduce morbidity and mortality in patient with chronic heart failure? Rationale and design of the diagnostic outcome trial in heart failure显示文摘 | Braunschweig F Ford I Conraads V | 2008 | Eur J Heart Fail2008，10，9： | 1 |
| 14 | Different immunohisto- chemical and uhrastructural phenotypes of squamous differentiation in bladder cancer显示文摘 | Gaisa N T Braunschweig T Reimer N | 2011 | Virchows Arch2011，458，3： | 1 |
| 15 | Positional identification of a regulatory mutation in IGF2 causing a major QTL effect on muscle growth in the pig显示文摘 | Van Laere A S Nguyen M Braunschweig M et a1 | 2003 | Nature2003，425，： | 1 |
| 16 | Pitfalls in TRAP assay in routine detection of malignancy in effusions显示文摘 | Braunschweig R Guilleret I Delacretaz F | 2001 | Diagn Cytopathol2001，25，4： | 1 |
| 17 | Assessment of gastric cancer:value of breathhold technique and tow-phase spiral CT显示文摘 | Hundt W Braunschweig R Reiser M | 1999 | Eur Radiol1999，9，1： | 1 |
| 18 | Altera-tions of the Wnt signaling pathway during the neoplastic progressionof Barrett's esophagus 显示文摘 | CLEMENT G BRAUNSCHWEIG R PASQUIER N | 2006 | Oncogene2006，25，21： | 1 |
| 19 | Pitfalls in TRAP assay in routine detection of malignancy in effusions显示文摘 | Braunschweig R Guilleret I Delacretaz F | 2001 | Diagn Cytopathol2001，25，4： | 1 |
| 20 | Maternal mid- pregnancy autoantibodies to fetal brain protein: the early markers for autism study 显示文摘 | Croen LA Braunschweig D Haapanen L | 2008 | Biol Psychiatry2008，64，： | 1 |
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