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| 1 | Effects of protein deprivation and re-feeding on P2X_2 receptors in enteric neurons显示文摘AIM:To investigate the effects of malnutrition and refeeding on the P2X2 receptor,nitric oxide synthase(NOS),calretinin,calbindin and choline acetyltransferase(ChAT) in neurons of the rat ileum.METHODS:We analyzed the co-localization,numbers and sizes of P2X2-expressing neurons in relation to NOS-immunoreactive(IR),calbindin-IR,ChAT-IR,and calretinin-IR neurons of the myenteric and submucosal plexus.The experimental groups consisted of:(1) rats maintained on normal feed throughout pregnancy until 42 d post-parturition(N);(2) rats deprived of protein throughout pregnancy and 42 d post-parturition(D);and(3) rats undernourished for 21 d post-parturition and then given a protein diet from days 22 to 42(DR).The myenteric and submucosal plexuses were evaluated by double labeling by immunohistochemical methods for P2X2 receptor,NOS,ChAT,calbindin and calretinin.RESULTS:We found similar P2X2 receptor immunoreactivity in the cytoplasm and surface membranes of myenteric and submucosal neurons from the N,D and DR groups.Double labeling of the myenteric plexus demonstrated that approximately 100% of NOS-IR,calbindin-IR,calretinin-IR and ChAT-IR neurons in all groups also expressed the P2X2 receptor.In the submucosal plexus,the calretinin-IR,ChAT-IR and calbindin-IR neurons were nearly all immunoreactive for the P2X2 receptor.In the myenteric plexus,there was a 19% increase in numbers per cm2 for P2X2 receptor-IR neurons,64% for NOS-IR,84% for calretinin-IR and 26% for ChAT-IR neurons in the D group.The spatial density of calbindin-IR neurons,however,did not differ among the three groups.The submucosal neuronal density increased for calbindin-IR,calretinin-IR and ChAT-IR neurons.The average size of neurons in the myenteric plexus neurons in the D group was less than that in the controls and,in the re-fed rats;there was a 34% reduction in size only for the calretinin-IR neurons.CONCLUSION:This work demonstrates that expression of the P2X2 receptor is present in inhibitory,intrinsic primary afferent,cholinergic secretomotor and vasomotor neurons.Undernutrition affected P2X2 receptor expression in the submucosal plexus,and neuronal and size.These changes were rescued in the re-fed rats. | Rúbia Misawa Priscila Azevedo Girotti Márcia Sanae Mizuno Edson Aparecido Liberti John Barton Furness Patricia Castelucci | 2010 | World Journal of Gastroenterology2010，16，29： | 2 |
| 2 | Oxygen sensing and plant acclimation to soil flooding显示文摘 | Horchani F R'bia O Aschi-Smiti S | 2011 | Int J Agric Res2011，6，3： | 1 |
| 3 | Treatment of cricopharyngeal dysfunction: a comparative pilot study 显示文摘 | Arenaz Bia B Olsson R Westin U | 2015 | BMC Res Notes2015，8，： | 1 |
| 4 | Partitioning of lysozyme in aqueous two-phase systems containing ethylene oxide-propylene oxide copolymer and potassium phosphates显示文摘 | Dembczyński R Bia as W Jankowski T | 2013 | Food and Bioproducts Processing2013，91，3： | 1 |
| 5 | Ischaemic injury to femoral head induces apoptotic and oncotic cell death显示文摘 | Kothapalli R Aya-ay JP Bia n H | | 0，，02： | 1 |
| 6 | Lysozyme extraction from hen egg white in an aqueous two-phase system composed of ethylene oxide-propylene oxide thermoseparating copolymer and potassium phosphate显示文摘 | Dembczyński R Bia as W Regulski K | 2010 | Process Biochemistry2010，45，3： | 1 |
| 7 | Quercetin in Lyotropic Liquid Crystalline Formulations: Physical, Chemical and Functional Stability显示文摘 | Fabiana T. M. C. Vicentini Rúbia Casagrande Waldiceu A. Verri Jr. Sandra R. Georgetti M. Vitória L. B. Bentley Maria J. V. Fonseca | 2008 | AAPS PharmSciTech2008，，2： | 1 |
| 8 | Abdominal vs vaginal hysterectomy: a comparative study of the postoperative quality of life and satisfaction显示文摘 | Agnaldo L. Silva-Filho Rogéria Andrade Werneck Rúbia Sena Magalh?es Andrezza V. Belo Sérgio A. Triginelli | 2006 | Archives of Gynecology and Obstetrics2006，，1： | 1 |
| 9 | Oxygen sensing and plant acclimation to soil flooding 显示文摘 | Horchani F R' bia O Aschi-Smiti S | 2011 | International Journal of Agricultural Research2011，6，3： | 1 |
| 10 | Oxygen sensing and plant ac- climation to soil flooding显示文摘 | Horchani F R'bia O Aschi-Smiti S | 2011 | International Journal of Agricultural Re- search2011，6，3： | 1 |
| 11 | Production of dry Lactobacillus rhamnosus GG preparations by spray drying and lyophilization in aqueous two-phase systems显示文摘 | Leja K Dembczy(n)ski R Bia(l)as W | | 0，，： | 1 |
| 12 | Cancer antigen 125 as a marker of ascites in patients with liver cirrhosis显示文摘 | JABKOWSKI M BIAKOWSKA J KOKTYSZ R | 2004 | Pol Merkur Lekarski2004，17，1： | 1 |
| 13 | A new technique for separation and purification of l-phenylalanine from fermentation liquid: flotation complexation extraction显示文摘 | Bia PY Donga H R Yua H B | 2008 | Sep Pur Tech2008，63，2： | 1 |
| 14 | Vibrio alginolyticus cellulites following coral injury 显示文摘 | Patterson T F Bell S R Bia F J | 1988 | Yale J Biol Med1988，61，6： | 1 |
| 15 | Sol-gel silica based networks with controlled chemical properties显示文摘 | Mansur Herman S Vasconcelos Wander L Lenza Rúbia S | 2000 | Journal of Non-Crystalline Solids2000，273，13： | 1 |
| 16 | Vibrio alginolyticus Cellulitis following coral injury 显示文摘 | Patterson T F Bell S R Bia F J | 1988 | The Yale Journal of Biology and Medicine1988，61，： | 1 |
| 17 | Giardia duodenalis : Pathological alterations in gerbils, Meriones unguiculatus , infected with different dosages of trophozoites显示文摘 | Núbia S. Araújo Maria J.S. Mundim Maria A. Gomes Rúbia M.R. Amorim Jo?o C. Viana Rodrigo P. Queiroz Marcos A. Rossi Márcia C. Cury | 2007 | Experimental Parasitology2007，，4： | 1 |
| 18 | Evaluation of the Antioxidant Activity of Different Flavonoids by the Chemiluminescence Method显示文摘 | Sandra R Georgetti Rúbia Casagrade Valéria M Di Mam bro | 2003 | AAPS PharmSci2003，5，2： | 1 |
| 19 | Oxygen sensing and plant acclimation to soil flooding 显示文摘 | Horchani F R'bia O Asehi-Smiti S | 2011 | International Journal of Agri-cultural Research2011，6，3： | 1 |
| 20 | Vegetation structure and edaphic factors in veredas reflect different conservation status in these threatened areas显示文摘Background:In Cerrado humid areas,veredas are considered as hygrophilous communities,marked by the presence of the Mauritia flexuosa palm tree emerging in the wetter zones.Changes in veredas ecosystems due to drought and changes in the water table,which represent important areas for biodiversity maintenance in the Brazilian cerrado,can lead to the emergence of new landscapes and ecosystems.Assuming that the veredas are changed in species composition due to anthropogenic factors,in this study,we evaluated the flora composition and described the current vegetation profile of two veredas under different disturbances levels that are located in the north of Minas Gerais State,Brazil.Results:Altogether,2,268 individuals of 91 species belonging to 36 families were recorded.Our results demonstrated that the studied veredas were floristically different in terms of the arboreous-shrubby species composition,richness,and diversity.Despite the hygrophilous forest formations and open areas in the two veredas present typical species from these environments(same species with high importance values),the drought in the water table caused an expansion of Cerrado species in the open areas and in the hygrophilous forest.Furthermore,in the hygrophilous forest there was an increase in the abundance of species that occur in less saturated soils.Conclusion:Our results demonstrate that the studied veredas are changing in species composition due to anthropogenic factors which leads to the drought of these formations(water balance effect).These anthropogenic effects cause a modification with the expansion and increase in the abundance of typical species of other areas of Cerrado and resistant to less saturated soils,leading to the veredas collapse.Finally,future studies should investigate other veredas on a broader scale to detect changes in the water table that lead to a consequent change in vegetation structure.In this way,we will have support for the conscientious management and conservation of these areas that represent equilibrium places for the Cerrado biome. | Yule Roberta Ferreira Nunes Camila Silveira Souza Islaine Francielly Pinheiro de Azevedo Odirlei Simôes de Oliveira Leidivan Almeida Frazão Rúbia Santos Fonseca Rubens Manoel dos Santos Walter Viana Neves | 2022 | Forest Ecosystems2022，9，3： | 0 |
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